Reviewed, UniProtKB/Swiss-Prot O33950 (CATA2_ACILW)

Names and origin

Protein names

Recommended name:
    Catechol 1,2-dioxygenase 2
    EC=1.13.11.1
Alternative name(s):
    1,2-CTD 2
    CDI2

Gene names

Name:

catA2

Organism

Acinetobacter lwoffii

Taxonomic identifier

28090 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can cleave 4-methyl-, 4-chloro-, and 3-methoxycatechol at lower rates than catechol, but has no activity with 4-nitrocatechol or protocatechuic acid.
Catalytic activity	Catechol + O2 = cis,cis-muconate.
Cofactor	Binds 1 Fe3+ ion per subunit.
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydro-2-furylacetate from catechol: step 1/3.
Subunit structure	Homodimer Potential.
Induction	By aniline.
Sequence similarities	Belongs to the intradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW catechol catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC ferric iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Catechol 1,2-dioxygenase 2		PRO_0000085082
Sites
	Metal binding	158	1	Iron By similarity
	Metal binding	192	1	Iron By similarity
	Metal binding	216	1	Iron By similarity
	Metal binding	218	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33950-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3F8B0C127F45A807	FASTA	275	30,399
	10 20 30 40 50 60 MNKQAIDALL QKINDSAINE GNPRTKQIVN RIVRDLFYTI EDLDVQPDEF WTALNYLGDA 70 80 90 100 110 120 GRSGELGLLA AGLGFEHFLD LRMDEAEAKA GVEGGTPRTI EGPLYVAGAP VSDGHARLDD 130 140 150 160 170 180 GTDPGQTLVM RGRVFGEDGK PLANALVEVW HANHLGNYSY FDKSQPAFNL RRSIRTDAEG 190 200 210 220 230 240 KYSFRSVVPV GYSVPPQGQT QLLLDQLGRH GHRPAHIHFF VSAPGFRKLT TQINIDGDPY 250 260 270 LWDDFAFATR DGLVPAVRQA EVRKANRTAW TVSSR

References

[1]

"Cloning and characterization of two catA genes in Acinetobacter lwoffii K24." Kim S.I., Leem S.-H., Choi J.-S., Chung Y.H., Kim S., Park Y.-M., Park Y.K., Lee Y.N., Ha K.-S. J. Bacteriol. 179:5226-5231(1997) [PubMed: 9260969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-30; 27-65 AND 229-247. Strain: K24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77659 Genomic DNA. Translation: AAC31767.1.
PIR	T46825.
3D structure databases
SMR	O33950. Positions 1-261.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.1. 96146.
Family and domain databases
InterPro	IPR007535. Catechol_dOase_N. IPR012801. Cchol_dOase_prob. IPR000627. Intradiol_dOase_C. IPR015889. Intradiol_dOase_core. [Graphical view]
Gene3D	G3DSA:2.60.130.10. Intradiol_dOase_core. 1 hit.
Pfam	PF00775. Dioxygenase_C. 1 hit. PF04444. Dioxygenase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02439. catechol_proteo. 1 hit.
PROSITE	PS00083. INTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATA2_ACILW

Accession

Primary (citable) accession number: O33950

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)