Reviewed, UniProtKB/Swiss-Prot O33948 (CATA1_ACILW)

Names and origin

Protein names

Recommended name:
    Catechol 1,2-dioxygenase 1
    EC=1.13.11.1
Alternative name(s):
    1,2-CTD 1
    CDI1

Gene names

Name:

catA1

Organism

Acinetobacter lwoffii

Taxonomic identifier

28090 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can cleave 4-methyl-, 4-chloro-, and 3-methoxycatechol at lower rates than catechol, but has no activity with 4-nitrocatechol or protocatechuic acid.
Catalytic activity	Catechol + O(2) = cis,cis-muconate.
Cofactor	Binds 1 Fe(3+) ion per subunit.
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydrofuran-2-acetate from catechol: step 1/3.
Subunit structure	Homodimer Potential.
Induction	By aniline.
Sequence similarities	Belongs to the intradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW catechol catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro ferric iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 311	310	Catechol 1,2-dioxygenase 1		PRO_0000085081
Sites
	Metal binding	164	1	Iron By similarity
	Metal binding	200	1	Iron By similarity
	Metal binding	224	1	Iron By similarity
	Metal binding	226	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33948-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BFA17B07716D41D3	FASTA	311	33,378
	10 20 30 40 50 60 MSIKVFGTKE VQDLLKAATN LEGKGGNARS KQIVHRLLSD LFKAIDDLDI TPDEVWAGVN 70 80 90 100 110 120 YLNKLGQDGE ATLLAAGSGL EKYLDIRLDA ADKAEGIEGG TPRTIEGPLY VAGATVHDGV 130 140 150 160 170 180 SKIDINPDED AGPLVIHGTV TGPDGKPVAG AVVECWHANS KGFYSHFDPT GAQSDFNLRG 190 200 210 220 230 240 AVKTGADGKY EFRTLMPVGY GCPPQGATQQ LLNVLGRHGN RPAHVHFFVS SDSARKLTTQ 250 260 270 280 290 300 FNIEGDPLIW DDFAYATREE LIPPVTEKKG GTALGLKADT YKDIEFNLTL TSLVKGKDNQ 310 VVHRLRAEVA A

References

[1]	"Cloning and characterization of two catA genes in Acinetobacter lwoffii K24." Kim S.I., Leem S.-H., Choi J.-S., Chung Y.H., Kim S., Park Y.-M., Park Y.K., Lee Y.N., Ha K.-S. J. Bacteriol. 179:5226-5231(1997) [PubMed: 9260969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-36; 45-72 AND 163-180. Strain: K24.
[2]	"Organization and transcriptional characterization of the cat1 gene cluster in Acinetobacter lwoffi K24." Kim S.I., Leem S.-H., Choi J.-S., Ha K.-S. Biochem. Biophys. Res. Commun. 243:289-294(1998) [PubMed: 9473520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29. Strain: K24.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U77658 Genomic DNA. Translation: AAC46228.1.
PIR	JC5943.
3D structure databases
HSSP	HSSP built from PDB template 1DMH based on UniProtKB P07773.
ModBase	Search...
Family and domain databases
InterPro	IPR007535. Catechol_dOase_N. IPR012801. Cchol_dOase_prob. IPR000627. Intradiol_dOase_C. IPR015889. Intradiol_dOase_core. [Graphical view]
Gene3D	G3DSA:2.60.130.10. Intradiol_dOase_core. 1 hit.
Pfam	PF00775. Dioxygenase_C. 1 hit. PF04444. Dioxygenase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02439. catechol_proteo. 1 hit.
PROSITE	PS00083. INTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATA1_ACILW

Accession

Primary (citable) accession number: O33948

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)