Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

LexA repressor

Gene

lexA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191For autocatalytic cleavage activityUniRule annotation
Active sitei156 – 1561For autocatalytic cleavage activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi28 – 4720H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, SOS response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Protein family/group databases

MEROPSiS24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexAUniRule annotation
Ordered Locus Names:TM_1082
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197LexA repressorPRO_0000170098Add
BLAST

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei83 – 842Cleavage; by autolysisUniRule annotation

Keywords - PTMi

Autocatalytic cleavage

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243274.TM1082.

Structurei

Secondary structure

1
197
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2217Combined sources
Helixi28 – 358Combined sources
Helixi39 – 5113Combined sources
Beta strandi54 – 563Combined sources
Helixi58 – 603Combined sources
Beta strandi66 – 694Combined sources
Beta strandi74 – 8310Combined sources
Helixi85 – 873Combined sources
Beta strandi89 – 9810Combined sources
Helixi101 – 1033Combined sources
Beta strandi110 – 1145Combined sources
Helixi121 – 1233Combined sources
Beta strandi130 – 1356Combined sources
Beta strandi144 – 1496Combined sources
Beta strandi152 – 16110Combined sources
Beta strandi164 – 1685Combined sources
Beta strandi177 – 1804Combined sources
Helixi181 – 1833Combined sources
Beta strandi185 – 19612Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K2ZX-ray1.37A/B3-197[»]
ProteinModelPortaliO33927.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33927.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DS7. Bacteria.
COG1974. LUCA.
InParanoidiO33927.
KOiK01356.
OMAiKQHELLM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA. 1 hit.
InterProiIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.

Sequencei

Sequence statusi: Complete.

O33927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDLTERQRK VLLFIEEFIE KNGYPPSVRE IARRFRITPR GALLHLIALE
60 70 80 90 100
KKGYIERKNG KPRALRISKS IRNKIPLIGE IRAGEKREAI EYLEDYIEIP
110 120 130 140 150
ESFLSSGYDH FLLKVKGESM IEEHICDGDL VLVRRQDWAQ NGDIVAAMVD
160 170 180 190
GEVTLKKFYQ RGDTVELRPA NREMSSMFFR AEKVKILGKV VGVFRKL
Length:197
Mass (Da):22,864
Last modified:January 1, 1998 - v1
Checksum:iA8093B0BB3D10BFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76417 Genomic DNA. Translation: AAB87145.1.
AE000512 Genomic DNA. Translation: AAD36159.1.
PIRiB72297.
RefSeqiNP_228888.1. NC_000853.1.
WP_004080401.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36159; AAD36159; TM_1082.
GeneIDi897745.
KEGGitma:TM1082.
tmi:THEMA_08945.
tmw:THMA_1104.
PATRICi23937093. VBITheMar51294_1095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76417 Genomic DNA. Translation: AAB87145.1.
AE000512 Genomic DNA. Translation: AAD36159.1.
PIRiB72297.
RefSeqiNP_228888.1. NC_000853.1.
WP_004080401.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K2ZX-ray1.37A/B3-197[»]
ProteinModelPortaliO33927.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1082.

Protein family/group databases

MEROPSiS24.001.

Protocols and materials databases

DNASUi897745.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36159; AAD36159; TM_1082.
GeneIDi897745.
KEGGitma:TM1082.
tmi:THEMA_08945.
tmw:THMA_1104.
PATRICi23937093. VBITheMar51294_1095.

Phylogenomic databases

eggNOGiENOG4105DS7. Bacteria.
COG1974. LUCA.
InParanoidiO33927.
KOiK01356.
OMAiKQHELLM.

Miscellaneous databases

EvolutionaryTraceiO33927.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA. 1 hit.
InterProiIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLEXA_THEMA
AccessioniPrimary (citable) accession number: O33927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

No consensus sequence similar to the SOS-box from E.coli or from B.subtilis was found upstream of the lexA gene, suggesting the presence of another target sequence specific for the Thermotogales.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.