Reviewed, UniProtKB/Swiss-Prot O33926 (GYRA_THEMA)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3
Alternative name(s):
    Topoisomerase II subunit A

Gene names

Name:	gyrA
Synonyms:	top2A
Ordered Locus Names:	TM_1084

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	804 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 804	804	DNA gyrase subunit A		PRO_0000145270
Sites
	Active site	119	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	91	1	Q → H in AAB87144. Ref.1
	Sequence conflict	153	1	P → T in AAB87144. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O33926-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 0ED421407D35949F	FASTA	804	90,448
	10 20 30 40 50 60 MPEILINKPV EDELVESYLL YSMSVIVGRA IPDVRDGLKP VQRRILYGMY ELGLKHNSPT 70 80 90 100 110 120 KKSARIVGEV MGKYHPHGDA PVYDALVRMA QPFTMRYPLI EGQGNFGSID RDPPAAMRYT 130 140 150 160 170 180 EARLTRLAEE MLEDIEKNTV NMIDNFDGTL KEPEVLPSKV PNLIINGASG IAVGMATNIP 190 200 210 220 230 240 PHNLSETVDA LIYLIDHPEA TVEELMQFIK GPDFPTGAVV VNASELKKVY EEGRGRIIVR 250 260 270 280 290 300 GKVHVEDGKR VKRIVITEIP YGVSKAGLIE QIAKIAKDDE SLPIRNIRDE SDKRGMRIVI 310 320 330 340 350 360 EIPKDANEEV IINNLYKRTA LQDYFNVQML VIDKHKRPRL MNLKGLMEAF LEHRFEVIRR 370 380 390 400 410 420 RARYEYEQYT RRAHVVEGLL KAARAIGVVV DIVRNSKDVE SARQSLMETL EITEEQAKAI 430 440 450 460 470 480 LDMRLSRLTS LEIENLQNEY SDLVRKISEV KEILEKDEKV KEIMKKEFLY LKQQYGDPRR 490 500 510 520 530 540 TEVTDQSIEY NEEELIVEED VVITLSHKGY LKSTPLNSYR SQKRGGKGIT VSKLSEDDEV 550 560 570 580 590 600 EFVVVAKNTS STLFITNLGR AYVLKNYQLE TTGRNTRGRH ITAFLNLEDT EKIVALASLN 610 620 630 640 650 660 GEGRDLVIAT KSGKIKRTAL KEFENATSNR GVRAIKIEPG DEIVSARVVN SEKETLIVAT 670 680 690 700 710 720 KMGMAIRFPV SDVRRMGRNA AGVQAIKLQP GDEVVSVDVI PPGEEGEILT VTEKGFGKRT 730 740 750 760 770 780 PVQLYRIQRR GGTGLRNISD VNKTGYVVAV RYVRGDEEIV VVTRNGMMIR FPVSEIGVIG 790 800 RVTKGVKLIE LGDDTISKVA VVKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Both DNA gyrase and reverse gyrase are present in the hyperthermophilic bacterium Thermotoga maritima." Guipaud O., Marguet E., Noll K.M., Bouthier de la Tour C., Forterre P. Proc. Natl. Acad. Sci. U.S.A. 94:10606-10611(1997) [PubMed: 9380682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U76417 Genomic DNA. Translation: AAB87144.1. AE000512 Genomic DNA. Translation: AAD36161.1.
PIR	D72297.
RefSeq	NP_228890.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Genome annotation databases
GeneID	897026.
GenomeReviews	Gene locus TM_1084 in contig AE000512_GR.
KEGG	tma:TM1084.
NMPDR	fig|243274.1.peg.1074.
TIGR	TM_1084.
Phylogenomic databases
HOGENOM	O33926.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_1084-MON.
BRENDA	5.99.1.3. 16699.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_THEMA

Accession

Primary (citable) accession number: O33926

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 3, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)