Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine--tRNA ligase

Gene

metG

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi125 – 1251ZincBy similarity
Metal bindingi128 – 1281ZincBy similarity
Metal bindingi146 – 1461ZincBy similarity
Metal bindingi149 – 1491ZincBy similarity
Binding sitei300 – 3001ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Synonyms:metS
Ordered Locus Names:TM_1085
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 629629Methionine--tRNA ligasePRO_0000139257Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi243274.TM1085.

Structurei

3D structure databases

ProteinModelPortaliO33925.
SMRiO33925. Positions 525-629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini529 – 629101tRNA-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi297 – 3015"KMSKS" region

Sequence similaritiesi

Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
InParanoidiO33925.
KOiK01874.
OMAiIAKEEPW.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFYITTPIY YVNSEPHIGS AYTTIVADII ARYKRFMGYD VFFLTGTDEH
60 70 80 90 100
GQKVLQAAQQ AGKDPQEFCD ELAEKFKRLW KELKITNDYF IRTTDEMHMK
110 120 130 140 150
TVQEFVAKMK ENGDVYKGIY KGWYCVPCET FWNEDEVIKE GEERFCPECK
160 170 180 190 200
RPVKWVEEEN YFFRLSKYRD SLLKYYEEHP DFVEPDFRRN EMLKILEGGL
210 220 230 240 250
KDLSITRTTF KWGVPMKDDP EHVIYVWVDA LINYISAIGY GWNDEMFNKW
260 270 280 290 300
WPADLHLIGK EINRFHSIIW PAMLMSVGLP LPKKVFAHGW LTVNGQKISK
310 320 330 340 350
SLGNAIDPRF FVKRYGNDVV RYYLIRDIMF GKDGDFSEER LVHRLNSDLA
360 370 380 390 400
NDYGNLLHRI TAMIKKYFNG RLPSPSAQEG FDSWLKERFF ETKDAYHELM
410 420 430 440 450
DSYRLTEALD KIWEFIADVN KYFNDTKPWI LGKEGNMERL GTVLYNSLEA
460 470 480 490 500
VFKVALMTLP VMPDTSEEVF RRVSFEEKPS KEHLENWGVL KPGSTVIHGE
510 520 530 540 550
PLFKKIDAKD FKKVVETVSA EQNAITIDDF SKVDLRIAKV LEAEKVPNSR
560 570 580 590 600
KLLRLIIDLG TEKRQIVAGI AEHYRPEELV GKLIVVVANL KPAKLMGIES
610 620
QGMLLAAKSG DTLRLLTVDG EITPGAKVS
Length:629
Mass (Da):73,004
Last modified:January 1, 1998 - v1
Checksum:iB0E0759F7C78ACEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76417 Genomic DNA. Translation: AAB87143.1.
AE000512 Genomic DNA. Translation: AAD36162.1.
PIRiE72297.
RefSeqiNP_228891.1. NC_000853.1.
WP_004080396.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36162; AAD36162; TM_1085.
GeneIDi897213.
KEGGitma:TM1085.
tmi:THEMA_08930.
tmw:THMA_1107.
PATRICi23937099. VBITheMar51294_1098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76417 Genomic DNA. Translation: AAB87143.1.
AE000512 Genomic DNA. Translation: AAD36162.1.
PIRiE72297.
RefSeqiNP_228891.1. NC_000853.1.
WP_004080396.1. NZ_CP011107.1.

3D structure databases

ProteinModelPortaliO33925.
SMRiO33925. Positions 525-629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1085.

Protocols and materials databases

DNASUi897213.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36162; AAD36162; TM_1085.
GeneIDi897213.
KEGGitma:TM1085.
tmi:THEMA_08930.
tmw:THMA_1107.
PATRICi23937099. VBITheMar51294_1098.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
InParanoidiO33925.
KOiK01874.
OMAiIAKEEPW.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_THEMA
AccessioniPrimary (citable) accession number: O33925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.