Reviewed, UniProtKB/Swiss-Prot O33924 (KPRS_CORAM)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:

prs

Organism

Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)

Taxonomic identifier

1697 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141129
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	O33924-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 11413BD2142AA24A	FASTA	317	34,836
	10 20 30 40 50 60 MSTQYLNSNL KVFSLNSNKE LAEQIAKHIG VGLGKCSVDR FSDGEVQINI EESIRGCDVF 70 80 90 100 110 120 IIQSTSFPVN EHIMELLIMI DALKRASAKT INIVIPYYGY ARQDRKARSR EPITSKLVAN 130 140 150 160 170 180 LLETAGATRV ITLDLHAPQI QGFFDIPIDH LMGVPILSDY FETKGLKDIV IVSPDHGGVT 190 200 210 220 230 240 RARKMADRLK APIAIIDKRR PRPNVSEVMN IIGNIEGKTA LLIDDIIDTA GTITLAANAL 250 260 270 280 290 300 VENGASEVYA CCTHPVLSGP AIERIQNSNI KELVVTNSIV LPEEKKIDKV HELSVAPLIG 310 EAIIRVYEEE SVSVLFN

References

[1]

"Cloning and expression of the gene encoding phosphoribosylpyrophosphate synthetase from Brevibacterium ammoniagenes KCTC1019." Kim M.K., Park B.C., Park J.H., Lim B.L., Lee D.-S. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6872 / DSM 20305 / KCTC 1019 / NCTC 2399.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U76387 Genomic DNA. Translation: AAB64352.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKR based on UniProtKB P14193.
SMR	O33924. Positions 2-316.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.6.1. 259964.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_CORAM

Accession

Primary (citable) accession number: O33924

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	January 1, 1998
Last modified:	September 22, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)