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Protein

DNA polymerase III subunit beta

Gene

dnaN

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit beta (EC:2.7.7.7)
Gene namesi
Name:dnaN
Ordered Locus Names:Mb0002
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 402402DNA polymerase III subunit betaPRO_0000105443Add
BLAST

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO33914.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000071793.
KOiK02338.
OMAiTHQIRLK.
OrthoDBiEOG65J53F.

Family and domain databases

InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

O33914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS
60 70 80 90 100
DNGLTISGFD YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVGV
110 120 130 140 150
HVEGNRVALT CGNARFSLPT MPVEDYPTLP TLPEETGLLP AELFAEAISQ
160 170 180 190 200
VAIAAGRDDT LPMLTGIRVE ILGETVVLAA TDRFRLAVRE LKWSASSPDI
210 220 230 240 250
EAAVLVPAKT LAEAAKAGIG GSDVRLSLGT GPGVGKDGLL GISGNGKRST
260 270 280 290 300
TRLLDAEFPK FRQLLPTEHT AVATMDVAEL IEAIKLVALV ADRGAQVRME
310 320 330 340 350
FADGSVRLSA GADDVGRAEE DLVVDYAGEP LTIAFNPTYL TDGLSSLRSE
360 370 380 390 400
RVSFGFTTAG KPALLRPVSG DDRPVAGLNG NGPFPAVSTD YVYLLMPVRL

PG
Length:402
Mass (Da):42,055
Last modified:July 11, 2003 - v2
Checksum:i830900921E3E03BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41235.1.
U75298 Genomic DNA. Translation: AAB81129.1.
RefSeqiNP_853672.1. NC_002945.3.
WP_010950321.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO41235; CDO41235; Mb0002.
GeneIDi1090744.
KEGGimbo:Mb0002.
PATRICi18001661. VBIMycBov88188_0002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41235.1.
U75298 Genomic DNA. Translation: AAB81129.1.
RefSeqiNP_853672.1. NC_002945.3.
WP_010950321.1. NC_002945.3.

3D structure databases

ProteinModelPortaliO33914.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO41235; CDO41235; Mb0002.
GeneIDi1090744.
KEGGimbo:Mb0002.
PATRICi18001661. VBIMycBov88188_0002.

Phylogenomic databases

HOGENOMiHOG000071793.
KOiK02338.
OMAiTHQIRLK.
OrthoDBiEOG65J53F.

Family and domain databases

InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. Qin M., Zachariah S., Madiraju M.V.V.S., Rajagopalan M.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
    Strain: BCG.

Entry informationi

Entry nameiDPO3B_MYCBO
AccessioniPrimary (citable) accession number: O33914
Secondary accession number(s): X2BDQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 11, 2003
Last modified: November 11, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.