O33897 (O33897_RHOMR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cellulase EMBL AAB65594.1
EC=3.2.1.4 EMBL AAB65594.1

Gene names

Name:

celA EMBL AAB65594.1

Organism

Rhodothermus marinus (Rhodothermus obamensis) EMBL AAB65594.1

Taxonomic identifier

29549 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidetes Order II. Incertae sedis › Rhodothermaceae › Rhodothermus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosidase EMBL AAB65594.1 Hydrolase
Technical term	3D-structure PDB 3B7M PDB 2BW8 PDB 1H0B PDB 2BWC PDB 2BWA
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O33897 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 94F197DB7D0D247A	FASTA	260	28,770
	10 20 30 40 50 60 MNVMRAVLVL SLLLLFGCDW LFPDGDNGKE PEPEPEPTVE LCGRWDARDV AGGRYRVINN 70 80 90 100 110 120 VWGAETAQCI EVGLETGNFT ITRADHDNGN NVAAYPAIYF GCHWAPARAI RDCAARAGAV 130 140 150 160 170 180 RRAHELDVTP ITTGRWNAAY DIWFSPVTNS GNGYSGGAEL MIWLNWNGGV MPGGSRVATV 190 200 210 220 230 240 ELAGATWEVW YADWDWNYIA YRRTTPTTSV SELDLKAFID DAVARGYIRP EWYLHAVETG 250 260 FELWEGGAGL RTADFSVTVQ

References

[1]	"Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12." Halldorsdottir S., Thorolfsdottir E.T., Spilliaert R., Johansson M., Thorbjarnardottir S.H., Palsdottir A., Hreggvidsson G.O., Kristjansson J.K., Holst O., Eggertsson G. Appl. Microbiol. Biotechnol. 49:277-284(1998) [PubMed: 9581291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ITI378 EMBL AAB65594.1.
[2]	"The structure of Rhodothermus marinus Cel12A, a highly thermostable family 12 endoglucanase, at 1.8 A resolution." Crennell S.J., Hreggvidsson G.O., Nordberg Karlsson E. J. Mol. Biol. 320:883-897(2002) [PubMed: 12095262] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 38-260.
[3]	"Transplantation of the active surface of a mesophile cellulase onto the structural scaffold of a homologous thermophile cellulase through engineering of a surface beta sheet." Kapoor D., Kumar V., Chandrayan S.K., Ahmed S., Sharma S., Datt M., Singh B., Karthikeyan S., Guptasarma P. Submitted (OCT-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 38-260.
[4]	"Dimerisation and an increase in active site aromatic groups as adaptations to high temperatures: X-ray solution scattering and substrate-bound crystal structures of Rhodothermus marinus endoglucanase Cel12A." Crennell S.J., Cook D., Minns A., Svergun D., Andersen R.L., Nordberg Karlsson E. J. Mol. Biol. 356:57-71(2006) [PubMed: 16343530] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS) OF 38-260.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U72637 Genomic DNA. Translation: AAB65594.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H0B	X-ray	1.80	A/B	38-260	[»]
2BW8	X-ray	1.54	A/B	38-260	[»]
2BWA	X-ray	1.68	A/B	38-260	[»]
2BWC	X-ray	2.15	A/B	38-260	[»]
3B7M	X-ray	2.10	A/B/C/D	38-260	[»]

ProteinModelPortal

O33897.

SMR

O33897. Positions 38-260.

ModBase

Search...

Protein family/group databases

CAZy

GH12. Glycoside Hydrolase Family 12.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013319. Glyco_hydro_11/12_cat.
IPR002594. Glyco_hydro_12.
[Graphical view]

Gene3D

G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.

Pfam

PF01670. Glyco_hydro_12. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O33897_RHOMR

Accession

Primary (citable) accession number: O33897

Entry history

Integrated into UniProtKB/TrEMBL:	January 1, 1998
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)