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Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene

fabA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-2-decenoyl-ACP and then its isomerization to Z-3-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).By similarity

Catalytic activityi

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei70By similarity1

GO - Molecular functioni

GO - Biological processi

  • unsaturated fatty acid biosynthetic process Source: PseudoCAP

Keywordsi

Molecular functionIsomerase, Lyase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-1640-MONOMER
UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA
Beta-hydroxydecanoyl thioester dehydrase
Trans-2-decenoyl-[acyl-carrier-protein] isomerase (EC:5.3.3.14)
Gene namesi
Name:fabA
Ordered Locus Names:PA1610
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi

Organism-specific databases

PseudoCAPiPA1610

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000916061 – 1713-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseAdd BLAST171

Proteomic databases

PaxDbiO33877

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi208964.PA1610

Structurei

Secondary structure

1171
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 16Combined sources8
Turni17 – 21Combined sources5
Turni31 – 33Combined sources3
Beta strandi37 – 45Combined sources9
Beta strandi53 – 59Combined sources7
Helixi66 – 69Combined sources4
Turni70 – 73Combined sources4
Helixi79 – 96Combined sources18
Beta strandi101 – 113Combined sources13
Beta strandi123 – 149Combined sources27
Beta strandi152 – 166Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B0BX-ray1.90A/B1-171[»]
4B0CX-ray2.70A/B/C/D/E1-171[»]
4B0IX-ray2.03A/B/C/D/E1-171[»]
4B0JX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-171[»]
4B8UX-ray2.76A/B/C/D/E1-171[»]
4CL6X-ray2.41A/B/C/D/E1-171[»]
4FQ9X-ray2.02A/B/C/D/E/F/G/H/I/J1-171[»]
ProteinModelPortaliO33877
SMRiO33877
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108RKI Bacteria
COG0764 LUCA
HOGENOMiHOG000277828
InParanoidiO33877
KOiK01716
OMAiFFDCHFK
PhylomeDBiO33877

Family and domain databases

CDDicd01287 FabA, 1 hit
HAMAPiMF_00405 FabA, 1 hit
InterProiView protein in InterPro
IPR010083 FabA
IPR013114 FabA_FabZ
IPR029069 HotDog_dom_sf
PANTHERiPTHR30272:SF8 PTHR30272:SF8, 1 hit
PfamiView protein in Pfam
PF07977 FabA, 1 hit
SUPFAMiSSF54637 SSF54637, 1 hit
TIGRFAMsiTIGR01749 fabA, 1 hit

Sequencei

Sequence statusi: Complete.

O33877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKQHAFTRE DLLRCSRGEL FGPGNAQLPA PNMLMIDRIV HISDVGGKYG
60 70 80 90 100
KGELVAELDI NPDLWFFACH FEGDPVMPGC LGLDAMWQLV GFYLGWQGNP
110 120 130 140 150
GRGRALGSGE VKFFGQVLPT AKKVTYNIHI KRTINRSLVL AIADGTVSVD
160 170
GREIYSAEGL RVGLFTSTDS F
Length:171
Mass (Da):18,748
Last modified:January 1, 1998 - v1
Checksum:i801E2B9C741A28FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70470 Genomic DNA Translation: AAC45619.1
AE004091 Genomic DNA Translation: AAG04999.1
PIRiD83443
RefSeqiNP_250301.1, NC_002516.2
WP_003087475.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG04999; AAG04999; PA1610
GeneIDi881984
KEGGipae:PA1610
PATRICifig|208964.12.peg.1670

Entry informationi

Entry nameiFABA_PSEAE
AccessioniPrimary (citable) accession number: O33877
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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