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O33877

- FABA_PSEAE

UniProt

O33877 - FABA_PSEAE

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Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene
fabA, PA1610
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length By similarity.UniRule annotation

Catalytic activityi

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.UniRule annotation
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.UniRule annotation
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701 By similarity

GO - Molecular functioni

  1. 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-HAMAP
  2. 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-EC
  3. trans-2-decenoyl-acyl-carrier-protein isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. unsaturated fatty acid biosynthetic process Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA
Beta-hydroxydecanoyl thioester dehydrase
Trans-2-decenoyl-[acyl-carrier-protein] isomerase (EC:5.3.3.14)
Gene namesi
Name:fabA
Ordered Locus Names:PA1610
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA1610.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1711713-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseUniRule annotationPRO_0000091606Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA1610.

Structurei

Secondary structure

1
171
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 168
Turni17 – 215
Turni31 – 333
Beta strandi37 – 459
Beta strandi53 – 597
Helixi66 – 694
Turni70 – 734
Helixi79 – 9618
Beta strandi101 – 11313
Beta strandi123 – 14927
Beta strandi152 – 16615

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0BX-ray1.90A/B1-171[»]
4B0CX-ray2.70A/B/C/D/E1-171[»]
4B0IX-ray2.03A/B/C/D/E1-171[»]
4B0JX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-171[»]
4B8UX-ray2.76A/B/C/D/E1-171[»]
4FQ9X-ray2.02A/B/C/D/E/F/G/H/I/J1-171[»]
ProteinModelPortaliO33877.
SMRiO33877. Positions 2-171.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0764.
HOGENOMiHOG000277828.
KOiK01716.
OMAiMGIADAT.
OrthoDBiEOG6VB6SH.
PhylomeDBiO33877.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.

Sequencei

Sequence statusi: Complete.

O33877-1 [UniParc]FASTAAdd to Basket

« Hide

MTKQHAFTRE DLLRCSRGEL FGPGNAQLPA PNMLMIDRIV HISDVGGKYG    50
KGELVAELDI NPDLWFFACH FEGDPVMPGC LGLDAMWQLV GFYLGWQGNP 100
GRGRALGSGE VKFFGQVLPT AKKVTYNIHI KRTINRSLVL AIADGTVSVD 150
GREIYSAEGL RVGLFTSTDS F 171
Length:171
Mass (Da):18,748
Last modified:January 1, 1998 - v1
Checksum:i801E2B9C741A28FC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U70470 Genomic DNA. Translation: AAC45619.1.
AE004091 Genomic DNA. Translation: AAG04999.1.
PIRiD83443.
RefSeqiNP_250301.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04999; AAG04999; PA1610.
GeneIDi881984.
KEGGipae:PA1610.
PATRICi19837569. VBIPseAer58763_1670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U70470 Genomic DNA. Translation: AAC45619.1 .
AE004091 Genomic DNA. Translation: AAG04999.1 .
PIRi D83443.
RefSeqi NP_250301.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4B0B X-ray 1.90 A/B 1-171 [» ]
4B0C X-ray 2.70 A/B/C/D/E 1-171 [» ]
4B0I X-ray 2.03 A/B/C/D/E 1-171 [» ]
4B0J X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-171 [» ]
4B8U X-ray 2.76 A/B/C/D/E 1-171 [» ]
4FQ9 X-ray 2.02 A/B/C/D/E/F/G/H/I/J 1-171 [» ]
ProteinModelPortali O33877.
SMRi O33877. Positions 2-171.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA1610.

Protocols and materials databases

DNASUi 881984.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG04999 ; AAG04999 ; PA1610 .
GeneIDi 881984.
KEGGi pae:PA1610.
PATRICi 19837569. VBIPseAer58763_1670.

Organism-specific databases

PseudoCAPi PA1610.

Phylogenomic databases

eggNOGi COG0764.
HOGENOMi HOG000277828.
KOi K01716.
OMAi MGIADAT.
OrthoDBi EOG6VB6SH.
PhylomeDBi O33877.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 3.10.129.10. 1 hit.
HAMAPi MF_00405. FabA.
InterProi IPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view ]
Pfami PF07977. FabA. 1 hit.
[Graphical view ]
SUPFAMi SSF54637. SSF54637. 1 hit.
TIGRFAMsi TIGR01749. fabA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Fatty acid biosynthesis in Pseudomonas aeruginosa: cloning and characterization of the fabAB operon encoding beta-hydroxyacyl-acyl carrier protein dehydratase (FabA) and beta-ketoacyl-acyl carrier protein synthase I (FabB)."
    Hoang T.T., Schweizer H.P.
    J. Bacteriol. 179:5326-5332(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiFABA_PSEAE
AccessioniPrimary (citable) accession number: O33877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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