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O33877

- FABA_PSEAE

UniProt

O33877 - FABA_PSEAE

Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene

fabA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length By similarity.By similarity

    Catalytic activityi

    A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.
    (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.
    Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei70 – 701By similarity

    GO - Molecular functioni

    1. 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-HAMAP
    2. 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-EC
    3. trans-2-decenoyl-acyl-carrier-protein isomerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. unsaturated fatty acid biosynthetic process Source: PseudoCAP

    Keywords - Molecular functioni

    Isomerase, Lyase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
    Alternative name(s):
    3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA
    Beta-hydroxydecanoyl thioester dehydrase
    Trans-2-decenoyl-[acyl-carrier-protein] isomerase (EC:5.3.3.14)
    Gene namesi
    Name:fabA
    Ordered Locus Names:PA1610
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA1610.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1711713-hydroxydecanoyl-[acyl-carrier-protein] dehydratasePRO_0000091606Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi208964.PA1610.

    Structurei

    Secondary structure

    1
    171
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 168
    Turni17 – 215
    Turni31 – 333
    Beta strandi37 – 459
    Beta strandi53 – 597
    Helixi66 – 694
    Turni70 – 734
    Helixi79 – 9618
    Beta strandi101 – 11313
    Beta strandi123 – 14927
    Beta strandi152 – 16615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4B0BX-ray1.90A/B1-171[»]
    4B0CX-ray2.70A/B/C/D/E1-171[»]
    4B0IX-ray2.03A/B/C/D/E1-171[»]
    4B0JX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-171[»]
    4B8UX-ray2.76A/B/C/D/E1-171[»]
    4FQ9X-ray2.02A/B/C/D/E/F/G/H/I/J1-171[»]
    ProteinModelPortaliO33877.
    SMRiO33877. Positions 2-171.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0764.
    HOGENOMiHOG000277828.
    KOiK01716.
    OMAiMGIADAT.
    OrthoDBiEOG6VB6SH.
    PhylomeDBiO33877.

    Family and domain databases

    Gene3Di3.10.129.10. 1 hit.
    HAMAPiMF_00405. FabA.
    InterProiIPR010083. FabA.
    IPR013114. FabA_FabZ.
    IPR029069. HotDog_dom.
    [Graphical view]
    PfamiPF07977. FabA. 1 hit.
    [Graphical view]
    SUPFAMiSSF54637. SSF54637. 1 hit.
    TIGRFAMsiTIGR01749. fabA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O33877-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKQHAFTRE DLLRCSRGEL FGPGNAQLPA PNMLMIDRIV HISDVGGKYG    50
    KGELVAELDI NPDLWFFACH FEGDPVMPGC LGLDAMWQLV GFYLGWQGNP 100
    GRGRALGSGE VKFFGQVLPT AKKVTYNIHI KRTINRSLVL AIADGTVSVD 150
    GREIYSAEGL RVGLFTSTDS F 171
    Length:171
    Mass (Da):18,748
    Last modified:January 1, 1998 - v1
    Checksum:i801E2B9C741A28FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70470 Genomic DNA. Translation: AAC45619.1.
    AE004091 Genomic DNA. Translation: AAG04999.1.
    PIRiD83443.
    RefSeqiNP_250301.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG04999; AAG04999; PA1610.
    GeneIDi881984.
    KEGGipae:PA1610.
    PATRICi19837569. VBIPseAer58763_1670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70470 Genomic DNA. Translation: AAC45619.1 .
    AE004091 Genomic DNA. Translation: AAG04999.1 .
    PIRi D83443.
    RefSeqi NP_250301.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4B0B X-ray 1.90 A/B 1-171 [» ]
    4B0C X-ray 2.70 A/B/C/D/E 1-171 [» ]
    4B0I X-ray 2.03 A/B/C/D/E 1-171 [» ]
    4B0J X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-171 [» ]
    4B8U X-ray 2.76 A/B/C/D/E 1-171 [» ]
    4FQ9 X-ray 2.02 A/B/C/D/E/F/G/H/I/J 1-171 [» ]
    ProteinModelPortali O33877.
    SMRi O33877. Positions 2-171.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA1610.

    Protocols and materials databases

    DNASUi 881984.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG04999 ; AAG04999 ; PA1610 .
    GeneIDi 881984.
    KEGGi pae:PA1610.
    PATRICi 19837569. VBIPseAer58763_1670.

    Organism-specific databases

    PseudoCAPi PA1610.

    Phylogenomic databases

    eggNOGi COG0764.
    HOGENOMi HOG000277828.
    KOi K01716.
    OMAi MGIADAT.
    OrthoDBi EOG6VB6SH.
    PhylomeDBi O33877.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .

    Family and domain databases

    Gene3Di 3.10.129.10. 1 hit.
    HAMAPi MF_00405. FabA.
    InterProi IPR010083. FabA.
    IPR013114. FabA_FabZ.
    IPR029069. HotDog_dom.
    [Graphical view ]
    Pfami PF07977. FabA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54637. SSF54637. 1 hit.
    TIGRFAMsi TIGR01749. fabA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Fatty acid biosynthesis in Pseudomonas aeruginosa: cloning and characterization of the fabAB operon encoding beta-hydroxyacyl-acyl carrier protein dehydratase (FabA) and beta-ketoacyl-acyl carrier protein synthase I (FabB)."
      Hoang T.T., Schweizer H.P.
      J. Bacteriol. 179:5326-5332(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiFABA_PSEAE
    AccessioniPrimary (citable) accession number: O33877
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3