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Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene

fabA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).By similarity

Catalytic activityi

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].

Pathway: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701By similarity

GO - Molecular functioni

GO - Biological processi

  • unsaturated fatty acid biosynthetic process Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA
Beta-hydroxydecanoyl thioester dehydrase
Trans-2-decenoyl-[acyl-carrier-protein] isomerase (EC:5.3.3.14)
Gene namesi
Name:fabA
Ordered Locus Names:PA1610
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1610.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1711713-hydroxydecanoyl-[acyl-carrier-protein] dehydratasePRO_0000091606Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi208964.PA1610.

Structurei

Secondary structure

1
171
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 168Combined sources
Turni17 – 215Combined sources
Turni31 – 333Combined sources
Beta strandi37 – 459Combined sources
Beta strandi53 – 597Combined sources
Helixi66 – 694Combined sources
Turni70 – 734Combined sources
Helixi79 – 9618Combined sources
Beta strandi101 – 11313Combined sources
Beta strandi123 – 14927Combined sources
Beta strandi152 – 16615Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0BX-ray1.90A/B1-171[»]
4B0CX-ray2.70A/B/C/D/E1-171[»]
4B0IX-ray2.03A/B/C/D/E1-171[»]
4B0JX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-171[»]
4B8UX-ray2.76A/B/C/D/E1-171[»]
4CL6X-ray2.41A/B/C/D/E1-171[»]
4FQ9X-ray2.02A/B/C/D/E/F/G/H/I/J1-171[»]
ProteinModelPortaliO33877.
SMRiO33877. Positions 2-171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0764.
HOGENOMiHOG000277828.
InParanoidiO33877.
KOiK01716.
OMAiFFDCHFK.
OrthoDBiEOG6VB6SH.
PhylomeDBiO33877.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.

Sequencei

Sequence statusi: Complete.

O33877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKQHAFTRE DLLRCSRGEL FGPGNAQLPA PNMLMIDRIV HISDVGGKYG
60 70 80 90 100
KGELVAELDI NPDLWFFACH FEGDPVMPGC LGLDAMWQLV GFYLGWQGNP
110 120 130 140 150
GRGRALGSGE VKFFGQVLPT AKKVTYNIHI KRTINRSLVL AIADGTVSVD
160 170
GREIYSAEGL RVGLFTSTDS F
Length:171
Mass (Da):18,748
Last modified:January 1, 1998 - v1
Checksum:i801E2B9C741A28FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70470 Genomic DNA. Translation: AAC45619.1.
AE004091 Genomic DNA. Translation: AAG04999.1.
PIRiD83443.
RefSeqiNP_250301.1. NC_002516.2.
WP_003087475.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04999; AAG04999; PA1610.
GeneIDi881984.
KEGGipae:PA1610.
PATRICi19837569. VBIPseAer58763_1670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70470 Genomic DNA. Translation: AAC45619.1.
AE004091 Genomic DNA. Translation: AAG04999.1.
PIRiD83443.
RefSeqiNP_250301.1. NC_002516.2.
WP_003087475.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0BX-ray1.90A/B1-171[»]
4B0CX-ray2.70A/B/C/D/E1-171[»]
4B0IX-ray2.03A/B/C/D/E1-171[»]
4B0JX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-171[»]
4B8UX-ray2.76A/B/C/D/E1-171[»]
4CL6X-ray2.41A/B/C/D/E1-171[»]
4FQ9X-ray2.02A/B/C/D/E/F/G/H/I/J1-171[»]
ProteinModelPortaliO33877.
SMRiO33877. Positions 2-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1610.

Protocols and materials databases

DNASUi881984.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04999; AAG04999; PA1610.
GeneIDi881984.
KEGGipae:PA1610.
PATRICi19837569. VBIPseAer58763_1670.

Organism-specific databases

PseudoCAPiPA1610.

Phylogenomic databases

eggNOGiCOG0764.
HOGENOMiHOG000277828.
InParanoidiO33877.
KOiK01716.
OMAiFFDCHFK.
OrthoDBiEOG6VB6SH.
PhylomeDBiO33877.

Enzyme and pathway databases

UniPathwayiUPA00094.

Miscellaneous databases

PROiO33877.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fatty acid biosynthesis in Pseudomonas aeruginosa: cloning and characterization of the fabAB operon encoding beta-hydroxyacyl-acyl carrier protein dehydratase (FabA) and beta-ketoacyl-acyl carrier protein synthase I (FabB)."
    Hoang T.T., Schweizer H.P.
    J. Bacteriol. 179:5326-5332(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiFABA_PSEAE
AccessioniPrimary (citable) accession number: O33877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.