O33853 (O33853_RHOSO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Endoglycoceramidase II EMBL AAB67050.1 EC=3.2.1.123 EMBL AAB67050.1
Organism	Rhodococcus sp. EMBL AAB67050.1
Taxonomic identifier	1831 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosidase EMBL AAB67050.1 Hydrolase
Technical term	3D-structure PDB 2OSX PDB 2OSY PDB 2OSW PDB 2OYM PDB 2OYL PDB 2OYK
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro endoglycosylceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	63 – 66	4	Galactose binding PDB 2OSX PDB 2OSY
	Region	155 – 156	2	Galactose binding PDB 2OSX PDB 2OSY
Sites
	Binding site	137	1	Galactose PDB 2OSX
	Binding site	382	1	Galactose PDB 2OSY
Amino acid modifications
	Glycosylation	351	1	O-linked (Glc...) PDB 2OSY

Sequences

	Sequence		Length	Mass (Da)
	O33853 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B52969F3D9E4D4E3	FASTA	490	52,751
	10 20 30 40 50 60 MRRTRLVSLI VTGSLVFGGG VAAAQSSLAA SGSGSGSGTA LTPSYLKDDD GRSLILRGFN 70 80 90 100 110 120 TASSAKSAPD GMPQFTEADL AREYADMGTN FVRFLISWRS VEPAPGVYDQ QYLDRVEDRV 130 140 150 160 170 180 GWYAERGYKV MLDMHQDVYS GAITPEGNSG NGAGAIGNGA PAWATYMDGL PVEPQPRWEL 190 200 210 220 230 240 YYIQPGVMRA FDNFWNTTGK HPELVEHYAK AWRAVADRFA DNDAVVAYDL MNEPFGGSLQ 250 260 270 280 290 300 GPAFEAGPLA AMYQRTTDAI RQVDQDTWVC VAPQAIGVNQ GLPSGLTKID DPRAGQQRIA 310 320 330 340 350 360 YCPHLYPLPL DIGDGHEGLA RTLTDVTIDA WRANTAHTAR VLGDVPIILG EFGLDTTLPG 370 380 390 400 410 420 ARDYIERVYG TAREMGAGVS YWSSDPGPWG PYLPDGTQTL LVDTLNKPYP RAVAGTPTEW 430 440 450 460 470 480 SSTSDRLQLT IEPDAAITAP TEIYLPEAGF PGDVHVEGAD VVGWDRQSRL LTVRTPADSG 490 NVTVTVTPAA

References

[1]	"Molecular cloning, expression, and sequence analysis of the endoglycoceramidase II gene from Rhodococcus species strain M-777." Izu H., Izumi Y., Kurome Y., Sano M., Kondo A., Kato I., Ito M. J. Biol. Chem. 272:19846-19850(1997) [PubMed: 9242646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: M-777 EMBL AAB67050.1.
[2]	"Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms." Caines M.E., Vaughan M.D., Tarling C.A., Hancock S.M., Warren R.A., Withers S.G., Strynadka N.C. J. Biol. Chem. 282:14300-14308(2007) [PubMed: 17329247] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) OF 31-490 IN COMPLEX WITH GALACTOSE, GLYCOSYLATION AT GLU-351.
[3]	"The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan a glycoside hydrolase endoglycoceramidase as a model system." Caines M.E., Hancock S.M., Tarling C.A., Wrodnigg T.M., Stick R.V., Stutz A.E., Vasella A., Withers S.G., Strynadka N.C. Angew. Chem. Int. Ed. 46:4474-4476(2007) [PubMed: 17487923] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 31-490.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U39554 Genomic DNA. Translation: AAB67050.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OSW	X-ray	1.60	A/B	31-490	[»]
2OSX	X-ray	1.10	A	31-490	[»]
2OSY	X-ray	2.10	A/B	31-490	[»]
2OYK	X-ray	1.50	A/B	31-490	[»]
2OYL	X-ray	1.80	A/B	31-490	[»]
2OYM	X-ray	1.86	A/B	31-490	[»]

ProteinModelPortal

O33853.

SMR

O33853. Positions 42-490.

ModBase

Search...

Protein family/group databases

CAZy

GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

3.2.1.123. 1423.

Family and domain databases

InterPro

IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.

Pfam

PF00150. Cellulase. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O33853_RHOSO

Accession

Primary (citable) accession number: O33853

Entry history

Integrated into UniProtKB/TrEMBL:	January 1, 1998
Last sequence update:	January 1, 1998
Last modified:	July 27, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)