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O33845 (DPOL_THEAG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase
EC=2.7.7.7
Alternative name(s):
Pol Tfu

Cleaved into the following 3 chains:

  1. Tag pol-1 intein
    Alternative name(s):
    Intein I
    Tsp-TY pol-1
  2. Tag pol-2 intein
    Alternative name(s):
    Intein II
    Tsp-TY pol-2
  3. Tag pol-3 intein
    Alternative name(s):
    Intein III
    Tsp-TY pol-3
Gene names
Name:pol
OrganismThermococcus aggregans
Taxonomic identifier110163 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length1829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the three intervening regions (inteins) followed by peptide ligation.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Contains 2 DOD-type homing endonuclease domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409DNA polymerase, 1st part Potential
PRO_0000007353
Chain410 – 769360Tag pol-1 intein Potential
PRO_0000007354
Chain770 – 85586DNA polymerase, 2nd part Potential
PRO_0000007355
Chain856 – 1392537Tag pol-2 intein Potential
PRO_0000007356
Chain1393 – 144149DNA polymerase, 3rd part Potential
PRO_0000007357
Chain1442 – 1598157Tag pol-3 intein Potential
PRO_0000007358
Chain1599 – 1829231DNA polymerase, 4th part Potential
PRO_0000007359

Regions

Domain527 – 668142DOD-type homing endonuclease 1
Domain1136 – 1269134DOD-type homing endonuclease 2

Sequences

Sequence LengthMass (Da)Tools
O33845 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A113A8BC57EB9CB3

FASTA1,829211,879
        10         20         30         40         50         60 
MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIDE IKAIKGERHG 

        70         80         90        100        110        120 
KIVRVVDAVK VKKKFLGRDV EVWKLIFEHP QDVPALRGKI REHPAVIDIY EYDIPFAKRY 

       130        140        150        160        170        180 
LIDKGLIPME GDEELKLMAF DIETFYHEGD EFGKGEIIMI SYADEEEARV ITWKNIDLPY 

       190        200        210        220        230        240 
VDVVSNEREM IKRFVQIVRE KDPDVLITYN GDNFDLPYLI KRAEKLGVTL LLGRDKEHPE 

       250        260        270        280        290        300 
PKIHRMGDSF AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI 

       310        320        330        340        350        360 
WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS TGNLVEWYLL 

       370        380        390        400        410        420 
RVAYERNELA PNKPDEEEYR RRLRTTYLGG YVKEPERGLW ENIAYLDFRC HPADTKVIVK 

       430        440        450        460        470        480 
GKGIVNISDV KEGDYILGID GWQRVKKVWK YHYEGKLINI NGLKCTPNHK VPVVTENDRQ 

       490        500        510        520        530        540 
TRIRDSLAKS FLSGKVKGKI ITTKLFEKIA EFEKNKPSEE EILKGELSGI ILAEGTLLRK 

       550        560        570        580        590        600 
DIEYFDSSRG KKRISHQYRV EITIGENEKE LLERILYIFD KLFGIRPSVK KKGDTNALKI 

       610        620        630        640        650        660 
TTAKKAVYLQ IEELLKNIES LYAPAVLRGF FERDATVNKI RSTIVVTQGT NNKWKIDIVA 

       670        680        690        700        710        720 
KLLDSLGIPY SRYEYKYIEN GKELTKHILE ITGRDGLILF QTLVGFISSE KNEALEKAIE 

       730        740        750        760        770        780 
VREMNRLKNN SFYNLSTFEV SSEYYKGEVY DLTLEGNPYY FANGILTHNS LYPSIIVTHN 

       790        800        810        820        830        840 
VSPDTLEREG CKNYDVAPIV GYKFCKDFPG FIPSILGELI TMRQEIKKKM KATIDPIEKK 

       850        860        870        880        890        900 
MLDYRQRAVK LLANSILPNE WLPIIENGEV KFVKIGEFID RYMEEQKDKV RTVDNTEVLE 

       910        920        930        940        950        960 
VDNIFAFSLN KESKKSEIKK VKALIRHKYK GEAYEVELNS GRKIHITRGH SLFTIRNGKI 

       970        980        990       1000       1010       1020 
KEIWGEEVKV GDLIIVPKKV KLNEKEAVIN IPELISKLPD EDTADVVMTT PVKGRKNFFK 

      1030       1040       1050       1060       1070       1080 
GMLRTLKWIF GEESKRIRTF NRYLFHLEEL GFVKLLPRGY EVTDWEGLKR YRQLYEKLVK 

      1090       1100       1110       1120       1130       1140 
NLRYNGNKRE YLVRFNDIKD SVSCFPRKEL EEWKIGTXKG FRXKCILKVD EDFGKFLGYY 

      1150       1160       1170       1180       1190       1200 
VSEGYAGAQK NKTGGMSYSV KLYNENPNVL KDMKNIAEKF FGKVRVGKNC VDIPKKMAYL 

      1210       1220       1230       1240       1250       1260 
LAKSLCGVTA ENKRIPSIIF DSSEPVRWAF LRAYFVGDGD IHPSKRLRLS TKSELLANQL 

      1270       1280       1290       1300       1310       1320 
VFLLNSLGVS SIKIGFDSGV YRVYINEDLP FLQTSRQKNT YYPNLIPKEV LEEIFGRKFQ 

      1330       1340       1350       1360       1370       1380 
KNITFEKFKE LADSGKLDKR KVKLLDFLLN GDIVLDRVKN VEKREYEGYV YDLSVEDNEN 

      1390       1400       1410       1420       1430       1440 
FLVGFGLLYA HNSYYGYMGY PKARWYSKEC AESVTAWGRH YIEMTIKEIE EKFGFKVLYA 

      1450       1460       1470       1480       1490       1500 
DSVTGDTEII VKRNGRIEFV PIEKLFERVD YRIGEKEYCI LEDVEALTLD NRGKLIWKKV 

      1510       1520       1530       1540       1550       1560 
PYVMRHRAKK KVYRIWITNS WYIDVTEDHS LIVAEDGLKE ARPMEIEGKS LIATKDDLSG 

      1570       1580       1590       1600       1610       1620 
VEYIKPHAIE EISYNGYVYD IEVEGTHRFF ANGILVHNTD GFYATIPGEK PETIKKKAKE 

      1630       1640       1650       1660       1670       1680 
FLKYINSKLP GLLELEYEGF YLRGFFVAKK RYAVIDEEGR ITTRGLEVVR RDWSEIAKET 

      1690       1700       1710       1720       1730       1740 
QAKVLEAILK EDSVEKAVEI VKDVVEEIAK YQVPLEKLVI HEQITKDLSE YKAIGPHVAI 

      1750       1760       1770       1780       1790       1800 
AKRLAAKGIK VRPGTIISYI VLRGSGKISD RVILLSEYDP KKHKYDPDYY IENQVLPAVL 

      1810       1820 
RILEAFGYRK EDLKYQSSKQ VGLDAWLKK

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References

[1]"Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY."
Niehaus F., Frey B., Antranikian G.
Gene 204:153-158(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: TY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y13030 Genomic DNA. Translation: CAA73475.1.

3D structure databases

ProteinModelPortalO33845.
SMRO33845. Positions 1-409, 766-855, 1392-1442, 1590-1828.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR004578. DNA-dir_DNA_pol_B_pol2.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 4 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. RNaseH_fold. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 2 hits.
TIGR00592. pol2. 2 hits.
PROSITEPS00116. DNA_POLYMERASE_B. False negative.
PS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDPOL_THEAG
AccessionPrimary (citable) accession number: O33845
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

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