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O33845

- DPOL_THEAG

UniProt

O33845 - DPOL_THEAG

Protein

DNA polymerase

Gene

pol

Organism
Thermococcus aggregans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. endonuclease activity Source: UniProtKB-KW
    4. nucleotide binding Source: InterPro

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase (EC:2.7.7.7)
    Alternative name(s):
    Pol Tfu
    Cleaved into the following 3 chains:
    Alternative name(s):
    Intein I
    Tsp-TY pol-1
    Alternative name(s):
    Intein II
    Tsp-TY pol-2
    Alternative name(s):
    Intein III
    Tsp-TY pol-3
    Gene namesi
    Name:pol
    OrganismiThermococcus aggregans
    Taxonomic identifieri110163 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 409409DNA polymerase, 1st partSequence AnalysisPRO_0000007353Add
    BLAST
    Chaini410 – 769360Tag pol-1 inteinSequence AnalysisPRO_0000007354Add
    BLAST
    Chaini770 – 85586DNA polymerase, 2nd partSequence AnalysisPRO_0000007355Add
    BLAST
    Chaini856 – 1392537Tag pol-2 inteinSequence AnalysisPRO_0000007356Add
    BLAST
    Chaini1393 – 144149DNA polymerase, 3rd partSequence AnalysisPRO_0000007357Add
    BLAST
    Chaini1442 – 1598157Tag pol-3 inteinSequence AnalysisPRO_0000007358Add
    BLAST
    Chaini1599 – 1829231DNA polymerase, 4th partSequence AnalysisPRO_0000007359Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the three intervening regions (inteins) followed by peptide ligation.

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Structurei

    3D structure databases

    ProteinModelPortaliO33845.
    SMRiO33845. Positions 1-409, 766-855, 1392-1442, 1590-1828.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini527 – 668142DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1136 – 1269134DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated
    Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di2.170.16.10. 4 hits.
    3.10.28.10. 3 hits.
    3.30.420.10. 1 hit.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 4 hits.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 3 hits.
    SM00306. HintN. 3 hits.
    SM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 5 hits.
    SSF53098. SSF53098. 1 hit.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsiTIGR01443. intein_Cterm. 3 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEiPS50818. INTEIN_C_TER. 3 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O33845-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIDE     50
    IKAIKGERHG KIVRVVDAVK VKKKFLGRDV EVWKLIFEHP QDVPALRGKI 100
    REHPAVIDIY EYDIPFAKRY LIDKGLIPME GDEELKLMAF DIETFYHEGD 150
    EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQIVRE 200
    KDPDVLITYN GDNFDLPYLI KRAEKLGVTL LLGRDKEHPE PKIHRMGDSF 250
    AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI 300
    WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS 350
    TGNLVEWYLL RVAYERNELA PNKPDEEEYR RRLRTTYLGG YVKEPERGLW 400
    ENIAYLDFRC HPADTKVIVK GKGIVNISDV KEGDYILGID GWQRVKKVWK 450
    YHYEGKLINI NGLKCTPNHK VPVVTENDRQ TRIRDSLAKS FLSGKVKGKI 500
    ITTKLFEKIA EFEKNKPSEE EILKGELSGI ILAEGTLLRK DIEYFDSSRG 550
    KKRISHQYRV EITIGENEKE LLERILYIFD KLFGIRPSVK KKGDTNALKI 600
    TTAKKAVYLQ IEELLKNIES LYAPAVLRGF FERDATVNKI RSTIVVTQGT 650
    NNKWKIDIVA KLLDSLGIPY SRYEYKYIEN GKELTKHILE ITGRDGLILF 700
    QTLVGFISSE KNEALEKAIE VREMNRLKNN SFYNLSTFEV SSEYYKGEVY 750
    DLTLEGNPYY FANGILTHNS LYPSIIVTHN VSPDTLEREG CKNYDVAPIV 800
    GYKFCKDFPG FIPSILGELI TMRQEIKKKM KATIDPIEKK MLDYRQRAVK 850
    LLANSILPNE WLPIIENGEV KFVKIGEFID RYMEEQKDKV RTVDNTEVLE 900
    VDNIFAFSLN KESKKSEIKK VKALIRHKYK GEAYEVELNS GRKIHITRGH 950
    SLFTIRNGKI KEIWGEEVKV GDLIIVPKKV KLNEKEAVIN IPELISKLPD 1000
    EDTADVVMTT PVKGRKNFFK GMLRTLKWIF GEESKRIRTF NRYLFHLEEL 1050
    GFVKLLPRGY EVTDWEGLKR YRQLYEKLVK NLRYNGNKRE YLVRFNDIKD 1100
    SVSCFPRKEL EEWKIGTXKG FRXKCILKVD EDFGKFLGYY VSEGYAGAQK 1150
    NKTGGMSYSV KLYNENPNVL KDMKNIAEKF FGKVRVGKNC VDIPKKMAYL 1200
    LAKSLCGVTA ENKRIPSIIF DSSEPVRWAF LRAYFVGDGD IHPSKRLRLS 1250
    TKSELLANQL VFLLNSLGVS SIKIGFDSGV YRVYINEDLP FLQTSRQKNT 1300
    YYPNLIPKEV LEEIFGRKFQ KNITFEKFKE LADSGKLDKR KVKLLDFLLN 1350
    GDIVLDRVKN VEKREYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY 1400
    PKARWYSKEC AESVTAWGRH YIEMTIKEIE EKFGFKVLYA DSVTGDTEII 1450
    VKRNGRIEFV PIEKLFERVD YRIGEKEYCI LEDVEALTLD NRGKLIWKKV 1500
    PYVMRHRAKK KVYRIWITNS WYIDVTEDHS LIVAEDGLKE ARPMEIEGKS 1550
    LIATKDDLSG VEYIKPHAIE EISYNGYVYD IEVEGTHRFF ANGILVHNTD 1600
    GFYATIPGEK PETIKKKAKE FLKYINSKLP GLLELEYEGF YLRGFFVAKK 1650
    RYAVIDEEGR ITTRGLEVVR RDWSEIAKET QAKVLEAILK EDSVEKAVEI 1700
    VKDVVEEIAK YQVPLEKLVI HEQITKDLSE YKAIGPHVAI AKRLAAKGIK 1750
    VRPGTIISYI VLRGSGKISD RVILLSEYDP KKHKYDPDYY IENQVLPAVL 1800
    RILEAFGYRK EDLKYQSSKQ VGLDAWLKK 1829
    Length:1,829
    Mass (Da):211,879
    Last modified:January 1, 1998 - v1
    Checksum:iA113A8BC57EB9CB3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13030 Genomic DNA. Translation: CAA73475.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13030 Genomic DNA. Translation: CAA73475.1 .

    3D structure databases

    ProteinModelPortali O33845.
    SMRi O33845. Positions 1-409, 766-855, 1392-1442, 1590-1828.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.170.16.10. 4 hits.
    3.10.28.10. 3 hits.
    3.30.420.10. 1 hit.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 4 hits.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 3 hits.
    SM00306. HintN. 3 hits.
    SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 5 hits.
    SSF53098. SSF53098. 1 hit.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsi TIGR01443. intein_Cterm. 3 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEi PS50818. INTEIN_C_TER. 3 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY."
      Niehaus F., Frey B., Antranikian G.
      Gene 204:153-158(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: TY.

    Entry informationi

    Entry nameiDPOL_THEAG
    AccessioniPrimary (citable) accession number: O33845
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3