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O33845

- DPOL_THEAG

UniProt

O33845 - DPOL_THEAG

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Protein
DNA polymerase
Gene
pol
Organism
Thermococcus aggregans
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
Pol Tfu
Cleaved into the following 3 chains:
Alternative name(s):
Intein I
Tsp-TY pol-1
Alternative name(s):
Intein II
Tsp-TY pol-2
Alternative name(s):
Intein III
Tsp-TY pol-3
Gene namesi
Name:pol
OrganismiThermococcus aggregans
Taxonomic identifieri110163 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409DNA polymerase, 1st part Reviewed prediction
PRO_0000007353Add
BLAST
Chaini410 – 769360Tag pol-1 intein Reviewed prediction
PRO_0000007354Add
BLAST
Chaini770 – 85586DNA polymerase, 2nd part Reviewed prediction
PRO_0000007355Add
BLAST
Chaini856 – 1392537Tag pol-2 intein Reviewed prediction
PRO_0000007356Add
BLAST
Chaini1393 – 144149DNA polymerase, 3rd part Reviewed prediction
PRO_0000007357Add
BLAST
Chaini1442 – 1598157Tag pol-3 intein Reviewed prediction
PRO_0000007358Add
BLAST
Chaini1599 – 1829231DNA polymerase, 4th part Reviewed prediction
PRO_0000007359Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the three intervening regions (inteins) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliO33845.
SMRiO33845. Positions 1-409, 766-855, 1392-1442, 1590-1828.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini527 – 668142DOD-type homing endonuclease 1
Add
BLAST
Domaini1136 – 1269134DOD-type homing endonuclease 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 4 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 5 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33845-1 [UniParc]FASTAAdd to Basket

« Hide

MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIDE     50
IKAIKGERHG KIVRVVDAVK VKKKFLGRDV EVWKLIFEHP QDVPALRGKI 100
REHPAVIDIY EYDIPFAKRY LIDKGLIPME GDEELKLMAF DIETFYHEGD 150
EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQIVRE 200
KDPDVLITYN GDNFDLPYLI KRAEKLGVTL LLGRDKEHPE PKIHRMGDSF 250
AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI 300
WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS 350
TGNLVEWYLL RVAYERNELA PNKPDEEEYR RRLRTTYLGG YVKEPERGLW 400
ENIAYLDFRC HPADTKVIVK GKGIVNISDV KEGDYILGID GWQRVKKVWK 450
YHYEGKLINI NGLKCTPNHK VPVVTENDRQ TRIRDSLAKS FLSGKVKGKI 500
ITTKLFEKIA EFEKNKPSEE EILKGELSGI ILAEGTLLRK DIEYFDSSRG 550
KKRISHQYRV EITIGENEKE LLERILYIFD KLFGIRPSVK KKGDTNALKI 600
TTAKKAVYLQ IEELLKNIES LYAPAVLRGF FERDATVNKI RSTIVVTQGT 650
NNKWKIDIVA KLLDSLGIPY SRYEYKYIEN GKELTKHILE ITGRDGLILF 700
QTLVGFISSE KNEALEKAIE VREMNRLKNN SFYNLSTFEV SSEYYKGEVY 750
DLTLEGNPYY FANGILTHNS LYPSIIVTHN VSPDTLEREG CKNYDVAPIV 800
GYKFCKDFPG FIPSILGELI TMRQEIKKKM KATIDPIEKK MLDYRQRAVK 850
LLANSILPNE WLPIIENGEV KFVKIGEFID RYMEEQKDKV RTVDNTEVLE 900
VDNIFAFSLN KESKKSEIKK VKALIRHKYK GEAYEVELNS GRKIHITRGH 950
SLFTIRNGKI KEIWGEEVKV GDLIIVPKKV KLNEKEAVIN IPELISKLPD 1000
EDTADVVMTT PVKGRKNFFK GMLRTLKWIF GEESKRIRTF NRYLFHLEEL 1050
GFVKLLPRGY EVTDWEGLKR YRQLYEKLVK NLRYNGNKRE YLVRFNDIKD 1100
SVSCFPRKEL EEWKIGTXKG FRXKCILKVD EDFGKFLGYY VSEGYAGAQK 1150
NKTGGMSYSV KLYNENPNVL KDMKNIAEKF FGKVRVGKNC VDIPKKMAYL 1200
LAKSLCGVTA ENKRIPSIIF DSSEPVRWAF LRAYFVGDGD IHPSKRLRLS 1250
TKSELLANQL VFLLNSLGVS SIKIGFDSGV YRVYINEDLP FLQTSRQKNT 1300
YYPNLIPKEV LEEIFGRKFQ KNITFEKFKE LADSGKLDKR KVKLLDFLLN 1350
GDIVLDRVKN VEKREYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY 1400
PKARWYSKEC AESVTAWGRH YIEMTIKEIE EKFGFKVLYA DSVTGDTEII 1450
VKRNGRIEFV PIEKLFERVD YRIGEKEYCI LEDVEALTLD NRGKLIWKKV 1500
PYVMRHRAKK KVYRIWITNS WYIDVTEDHS LIVAEDGLKE ARPMEIEGKS 1550
LIATKDDLSG VEYIKPHAIE EISYNGYVYD IEVEGTHRFF ANGILVHNTD 1600
GFYATIPGEK PETIKKKAKE FLKYINSKLP GLLELEYEGF YLRGFFVAKK 1650
RYAVIDEEGR ITTRGLEVVR RDWSEIAKET QAKVLEAILK EDSVEKAVEI 1700
VKDVVEEIAK YQVPLEKLVI HEQITKDLSE YKAIGPHVAI AKRLAAKGIK 1750
VRPGTIISYI VLRGSGKISD RVILLSEYDP KKHKYDPDYY IENQVLPAVL 1800
RILEAFGYRK EDLKYQSSKQ VGLDAWLKK 1829
Length:1,829
Mass (Da):211,879
Last modified:January 1, 1998 - v1
Checksum:iA113A8BC57EB9CB3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13030 Genomic DNA. Translation: CAA73475.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13030 Genomic DNA. Translation: CAA73475.1 .

3D structure databases

ProteinModelPortali O33845.
SMRi O33845. Positions 1-409, 766-855, 1392-1442, 1590-1828.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 4 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 5 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY."
    Niehaus F., Frey B., Antranikian G.
    Gene 204:153-158(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: TY.

Entry informationi

Entry nameiDPOL_THEAG
AccessioniPrimary (citable) accession number: O33845
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi