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O33845

- DPOL_THEAG

UniProt

O33845 - DPOL_THEAG

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Protein

DNA polymerase

Gene

pol

Organism
Thermococcus aggregans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Protein family/group databases

MEROPSiN10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
Pol Tfu
Cleaved into the following 3 chains:
Alternative name(s):
Intein I
Tsp-TY pol-1
Alternative name(s):
Intein II
Tsp-TY pol-2
Alternative name(s):
Intein III
Tsp-TY pol-3
Gene namesi
Name:pol
OrganismiThermococcus aggregans
Taxonomic identifieri110163 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409DNA polymerase, 1st partSequence AnalysisPRO_0000007353Add
BLAST
Chaini410 – 769360Tag pol-1 inteinSequence AnalysisPRO_0000007354Add
BLAST
Chaini770 – 85586DNA polymerase, 2nd partSequence AnalysisPRO_0000007355Add
BLAST
Chaini856 – 1392537Tag pol-2 inteinSequence AnalysisPRO_0000007356Add
BLAST
Chaini1393 – 144149DNA polymerase, 3rd partSequence AnalysisPRO_0000007357Add
BLAST
Chaini1442 – 1598157Tag pol-3 inteinSequence AnalysisPRO_0000007358Add
BLAST
Chaini1599 – 1829231DNA polymerase, 4th partSequence AnalysisPRO_0000007359Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the three intervening regions (inteins) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliO33845.
SMRiO33845. Positions 1-409, 766-855, 1392-1442, 1590-1828.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini527 – 668142DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
BLAST
Domaini1136 – 1269134DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated
Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 4 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 5 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33845-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIDE
60 70 80 90 100
IKAIKGERHG KIVRVVDAVK VKKKFLGRDV EVWKLIFEHP QDVPALRGKI
110 120 130 140 150
REHPAVIDIY EYDIPFAKRY LIDKGLIPME GDEELKLMAF DIETFYHEGD
160 170 180 190 200
EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQIVRE
210 220 230 240 250
KDPDVLITYN GDNFDLPYLI KRAEKLGVTL LLGRDKEHPE PKIHRMGDSF
260 270 280 290 300
AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI
310 320 330 340 350
WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS
360 370 380 390 400
TGNLVEWYLL RVAYERNELA PNKPDEEEYR RRLRTTYLGG YVKEPERGLW
410 420 430 440 450
ENIAYLDFRC HPADTKVIVK GKGIVNISDV KEGDYILGID GWQRVKKVWK
460 470 480 490 500
YHYEGKLINI NGLKCTPNHK VPVVTENDRQ TRIRDSLAKS FLSGKVKGKI
510 520 530 540 550
ITTKLFEKIA EFEKNKPSEE EILKGELSGI ILAEGTLLRK DIEYFDSSRG
560 570 580 590 600
KKRISHQYRV EITIGENEKE LLERILYIFD KLFGIRPSVK KKGDTNALKI
610 620 630 640 650
TTAKKAVYLQ IEELLKNIES LYAPAVLRGF FERDATVNKI RSTIVVTQGT
660 670 680 690 700
NNKWKIDIVA KLLDSLGIPY SRYEYKYIEN GKELTKHILE ITGRDGLILF
710 720 730 740 750
QTLVGFISSE KNEALEKAIE VREMNRLKNN SFYNLSTFEV SSEYYKGEVY
760 770 780 790 800
DLTLEGNPYY FANGILTHNS LYPSIIVTHN VSPDTLEREG CKNYDVAPIV
810 820 830 840 850
GYKFCKDFPG FIPSILGELI TMRQEIKKKM KATIDPIEKK MLDYRQRAVK
860 870 880 890 900
LLANSILPNE WLPIIENGEV KFVKIGEFID RYMEEQKDKV RTVDNTEVLE
910 920 930 940 950
VDNIFAFSLN KESKKSEIKK VKALIRHKYK GEAYEVELNS GRKIHITRGH
960 970 980 990 1000
SLFTIRNGKI KEIWGEEVKV GDLIIVPKKV KLNEKEAVIN IPELISKLPD
1010 1020 1030 1040 1050
EDTADVVMTT PVKGRKNFFK GMLRTLKWIF GEESKRIRTF NRYLFHLEEL
1060 1070 1080 1090 1100
GFVKLLPRGY EVTDWEGLKR YRQLYEKLVK NLRYNGNKRE YLVRFNDIKD
1110 1120 1130 1140 1150
SVSCFPRKEL EEWKIGTXKG FRXKCILKVD EDFGKFLGYY VSEGYAGAQK
1160 1170 1180 1190 1200
NKTGGMSYSV KLYNENPNVL KDMKNIAEKF FGKVRVGKNC VDIPKKMAYL
1210 1220 1230 1240 1250
LAKSLCGVTA ENKRIPSIIF DSSEPVRWAF LRAYFVGDGD IHPSKRLRLS
1260 1270 1280 1290 1300
TKSELLANQL VFLLNSLGVS SIKIGFDSGV YRVYINEDLP FLQTSRQKNT
1310 1320 1330 1340 1350
YYPNLIPKEV LEEIFGRKFQ KNITFEKFKE LADSGKLDKR KVKLLDFLLN
1360 1370 1380 1390 1400
GDIVLDRVKN VEKREYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY
1410 1420 1430 1440 1450
PKARWYSKEC AESVTAWGRH YIEMTIKEIE EKFGFKVLYA DSVTGDTEII
1460 1470 1480 1490 1500
VKRNGRIEFV PIEKLFERVD YRIGEKEYCI LEDVEALTLD NRGKLIWKKV
1510 1520 1530 1540 1550
PYVMRHRAKK KVYRIWITNS WYIDVTEDHS LIVAEDGLKE ARPMEIEGKS
1560 1570 1580 1590 1600
LIATKDDLSG VEYIKPHAIE EISYNGYVYD IEVEGTHRFF ANGILVHNTD
1610 1620 1630 1640 1650
GFYATIPGEK PETIKKKAKE FLKYINSKLP GLLELEYEGF YLRGFFVAKK
1660 1670 1680 1690 1700
RYAVIDEEGR ITTRGLEVVR RDWSEIAKET QAKVLEAILK EDSVEKAVEI
1710 1720 1730 1740 1750
VKDVVEEIAK YQVPLEKLVI HEQITKDLSE YKAIGPHVAI AKRLAAKGIK
1760 1770 1780 1790 1800
VRPGTIISYI VLRGSGKISD RVILLSEYDP KKHKYDPDYY IENQVLPAVL
1810 1820
RILEAFGYRK EDLKYQSSKQ VGLDAWLKK
Length:1,829
Mass (Da):211,879
Last modified:January 1, 1998 - v1
Checksum:iA113A8BC57EB9CB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13030 Genomic DNA. Translation: CAA73475.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13030 Genomic DNA. Translation: CAA73475.1 .

3D structure databases

ProteinModelPortali O33845.
SMRi O33845. Positions 1-409, 766-855, 1392-1442, 1590-1828.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi N10.007.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 4 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 5 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY."
    Niehaus F., Frey B., Antranikian G.
    Gene 204:153-158(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: TY.

Entry informationi

Entry nameiDPOL_THEAG
AccessioniPrimary (citable) accession number: O33845
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3