Reviewed, UniProtKB/Swiss-Prot O33844 (DMA_TREPA)

Names and origin

Protein names

Recommended name:
    DNA adenine methylase
    EC=2.1.1.72
Alternative name(s):
    M.TpaI
    Deoxyadenosyl-methyltransferase
    DNA adenine methyltransferase

Gene names

Name:	dam
Ordered Locus Names:	TP_0810

Organism

Treponema pallidum [Complete proteome] [HAMAP]

Taxonomic identifier

160 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair, DNA replication, and gene expression.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	DNA adenine methylase		PRO_0000087998
Sites
	Binding site	13	1	S-adenosyl-L-methionine By similarity
	Binding site	17	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	64	1	S-adenosyl-L-methionine By similarity
	Binding site	212	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33844-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 62D2155EAE22DB90	FASTA	303	33,919
	10 20 30 40 50 60 MSRSDTARPF VKWAGGKRAL APTLFAHMPQ TFGSYFEPFV GGGALFWHLC ACTRVRLHDI 70 80 90 100 110 120 YLSDINWPLL CAYAAVRDRV EELIVRVGQH IACHTPTYYR LARRKFAVCE HPLEVAALFL 130 140 150 160 170 180 YLNRSCYNGL YRVNKAGQFN VPLGRAAPAS PFLNTTAPTP RSTQPAAQVG HLAIRIDEEN 190 200 210 220 230 240 LRSCARALAN TTLNCQHFSC IQPARGDFVY LDPPYLGTFS AYDKTGFDRA AHESLAAFCM 250 260 270 280 290 300 HLDARGVLFM LSNSDCPEVR AWYRPFRVQQ LNAPRCIARS AHARGKRCEV LITNYPCADT ATP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a Treponema pallidum subsp. pallidum gene encoding a DNA adenine methyltransferase." Stamm L.V., Greene S.R., Barnes N.Y., Bergen H.L., Hardham J.M. FEMS Microbiol. Lett. 155:115-119(1997) [PubMed: 9345771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nichols.
[2]	"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed: 9665876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF006263 Genomic DNA. Translation: AAB82782.1. AE000520 Genomic DNA. Translation: AAC65779.1.
PIR	H71277.
RefSeq	NP_219247.1.
3D structure databases
HSSP	HSSP built from PDB template 2DPM based on UniProtKB P04043.
ModBase	Search...
Protein-protein interaction databases
IntAct	O33844. 1 interaction.
Genome annotation databases
GeneID	2610874.
GenomeReviews	Gene locus TP_0810 in contig AE000520_GR.
KEGG	tpa:TP0810.
NMPDR	fig|243276.1.peg.806.
TIGR	TP_0810.
Phylogenomic databases
HOGENOM	O33844.
OMA	WAGGKFR.
Enzyme and pathway databases
BioCyc	TPAL243276:TP_0810-MON.
BRENDA	2.1.1.72. 142600.
Family and domain databases
InterPro	IPR002294. D12N6_MeTrfase. IPR012326. Dam. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA_TREPA

Accession

Primary (citable) accession number: O33844

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)