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Protein

DNA adenine methylase

Gene

dam

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair, DNA replication, and gene expression.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131S-adenosyl-L-methionineBy similarity
Binding sitei17 – 171S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei64 – 641S-adenosyl-L-methionineBy similarity
Binding sitei212 – 2121S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTPAL243276:GC1H-858-MONOMER.

Protein family/group databases

REBASEi3131. M.TpaI.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.TpaI
Gene namesi
Name:dam
Ordered Locus Names:TP_0810
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000000811: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303DNA adenine methylasePRO_0000087998Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiO33844. 1 interaction.
STRINGi243276.TP0810.

Structurei

3D structure databases

ProteinModelPortaliO33844.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSDTARPF VKWAGGKRAL APTLFAHMPQ TFGSYFEPFV GGGALFWHLC
60 70 80 90 100
ACTRVRLHDI YLSDINWPLL CAYAAVRDRV EELIVRVGQH IACHTPTYYR
110 120 130 140 150
LARRKFAVCE HPLEVAALFL YLNRSCYNGL YRVNKAGQFN VPLGRAAPAS
160 170 180 190 200
PFLNTTAPTP RSTQPAAQVG HLAIRIDEEN LRSCARALAN TTLNCQHFSC
210 220 230 240 250
IQPARGDFVY LDPPYLGTFS AYDKTGFDRA AHESLAAFCM HLDARGVLFM
260 270 280 290 300
LSNSDCPEVR AWYRPFRVQQ LNAPRCIARS AHARGKRCEV LITNYPCADT

ATP
Length:303
Mass (Da):33,919
Last modified:January 1, 1998 - v1
Checksum:i62D2155EAE22DB90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006263 Genomic DNA. Translation: AAB82782.1.
AE000520 Genomic DNA. Translation: AAC65779.1.
PIRiH71277.
RefSeqiNP_219247.1. NC_000919.1.
WP_010882255.1. NC_021490.2.
YP_008091637.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65779; AAC65779; TP_0810.
GeneIDi15852101.
2610874.
KEGGitpa:TP0810.
tpw:TPANIC_0810.
PATRICi20531719. VBITrePal57110_0857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006263 Genomic DNA. Translation: AAB82782.1.
AE000520 Genomic DNA. Translation: AAC65779.1.
PIRiH71277.
RefSeqiNP_219247.1. NC_000919.1.
WP_010882255.1. NC_021490.2.
YP_008091637.1. NC_021490.2.

3D structure databases

ProteinModelPortaliO33844.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO33844. 1 interaction.
STRINGi243276.TP0810.

Protein family/group databases

REBASEi3131. M.TpaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC65779; AAC65779; TP_0810.
GeneIDi15852101.
2610874.
KEGGitpa:TP0810.
tpw:TPANIC_0810.
PATRICi20531719. VBITrePal57110_0857.

Phylogenomic databases

eggNOGiCOG0338.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.

Enzyme and pathway databases

BioCyciTPAL243276:GC1H-858-MONOMER.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a Treponema pallidum subsp. pallidum gene encoding a DNA adenine methyltransferase."
    Stamm L.V., Greene S.R., Barnes N.Y., Bergen H.L., Hardham J.M.
    FEMS Microbiol. Lett. 155:115-119(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Nichols.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nichols.

Entry informationi

Entry nameiDMA_TREPA
AccessioniPrimary (citable) accession number: O33844
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.