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O33844

- DMA_TREPA

UniProt

O33844 - DMA_TREPA

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Protein

DNA adenine methylase

Gene

dam

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair, DNA replication, and gene expression.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131S-adenosyl-L-methionineBy similarity
Binding sitei17 – 171S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei64 – 641S-adenosyl-L-methionineBy similarity
Binding sitei212 – 2121S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTPAL243276:GC1H-858-MONOMER.

Protein family/group databases

REBASEi3131. M.TpaI.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.TpaI
Gene namesi
Name:dam
Ordered Locus Names:TP_0810
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000000811: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303DNA adenine methylasePRO_0000087998Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiO33844. 1 interaction.
STRINGi243276.TP0810.

Structurei

3D structure databases

ProteinModelPortaliO33844.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
KOiK06223.
OMAiNSDSEFI.
OrthoDBiEOG6CS08V.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33844-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRSDTARPF VKWAGGKRAL APTLFAHMPQ TFGSYFEPFV GGGALFWHLC
60 70 80 90 100
ACTRVRLHDI YLSDINWPLL CAYAAVRDRV EELIVRVGQH IACHTPTYYR
110 120 130 140 150
LARRKFAVCE HPLEVAALFL YLNRSCYNGL YRVNKAGQFN VPLGRAAPAS
160 170 180 190 200
PFLNTTAPTP RSTQPAAQVG HLAIRIDEEN LRSCARALAN TTLNCQHFSC
210 220 230 240 250
IQPARGDFVY LDPPYLGTFS AYDKTGFDRA AHESLAAFCM HLDARGVLFM
260 270 280 290 300
LSNSDCPEVR AWYRPFRVQQ LNAPRCIARS AHARGKRCEV LITNYPCADT

ATP
Length:303
Mass (Da):33,919
Last modified:January 1, 1998 - v1
Checksum:i62D2155EAE22DB90
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006263 Genomic DNA. Translation: AAB82782.1.
AE000520 Genomic DNA. Translation: AAC65779.1.
PIRiH71277.
RefSeqiNP_219247.1. NC_000919.1.
WP_010882255.1. NC_021490.2.
YP_008091637.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65779; AAC65779; TP_0810.
GeneIDi15852101.
2610874.
KEGGitpa:TP0810.
tpw:TPANIC_0810.
PATRICi20531719. VBITrePal57110_0857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006263 Genomic DNA. Translation: AAB82782.1 .
AE000520 Genomic DNA. Translation: AAC65779.1 .
PIRi H71277.
RefSeqi NP_219247.1. NC_000919.1.
WP_010882255.1. NC_021490.2.
YP_008091637.1. NC_021490.2.

3D structure databases

ProteinModelPortali O33844.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O33844. 1 interaction.
STRINGi 243276.TP0810.

Protein family/group databases

REBASEi 3131. M.TpaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC65779 ; AAC65779 ; TP_0810 .
GeneIDi 15852101.
2610874.
KEGGi tpa:TP0810.
tpw:TPANIC_0810.
PATRICi 20531719. VBITrePal57110_0857.

Phylogenomic databases

eggNOGi COG0338.
KOi K06223.
OMAi NSDSEFI.
OrthoDBi EOG6CS08V.

Enzyme and pathway databases

BioCyci TPAL243276:GC1H-858-MONOMER.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 2 hits.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a Treponema pallidum subsp. pallidum gene encoding a DNA adenine methyltransferase."
    Stamm L.V., Greene S.R., Barnes N.Y., Bergen H.L., Hardham J.M.
    FEMS Microbiol. Lett. 155:115-119(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Nichols.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nichols.

Entry informationi

Entry nameiDMA_TREPA
AccessioniPrimary (citable) accession number: O33844
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3