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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Thermotoga maritima
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Cofactori

adenosylcob(III)alaminUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26TTPCombined sources1
Binding sitei91ADPCombined sources1
Binding sitei91CDPCombined sources1
Binding sitei91TTPCombined sources1
Binding sitei91UDPCombined sources1
Binding sitei133ADP; via carbonyl oxygenCombined sources1
Binding sitei133CDP; via carbonyl oxygenCombined sources1
Binding sitei133UDP; via carbonyl oxygenCombined sources1
Binding sitei158TTPCombined sources1
Binding sitei162CDP; via amide nitrogenCombined sources1
Binding sitei171TTPCombined sources1
Binding sitei185TTPCombined sources1
Binding sitei202ADPCombined sources1
Binding sitei203CDPCombined sources1
Binding sitei203UDPCombined sources1
Binding sitei210ADP; via amide nitrogenCombined sources1
Binding sitei324TTPCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 143TTPCombined sources3
Nucleotide bindingi178 – 179TTPCombined sources2
Nucleotide bindingi200 – 202TTPCombined sources3
Nucleotide bindingi207 – 208CDPCombined sources2
Nucleotide bindingi320 – 324ADPCombined sources5
Nucleotide bindingi320 – 324CDPCombined sources5
Nucleotide bindingi320 – 324UDPCombined sources5
Nucleotide bindingi490 – 494ADPCombined sources5
Nucleotide bindingi490 – 494CDPCombined sources5
Nucleotide bindingi490 – 494TTPCombined sources5
Nucleotide bindingi490 – 494UDPCombined sources5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotation
Biological processDNA synthesisUniRule annotation
LigandCobalaminUniRule annotation, Cobalt, Nucleotide-bindingUniRule annotationCombined sources

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15779.
BRENDAi1.17.4.1. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductaseUniRule annotation (EC:1.17.4.1UniRule annotation)
Gene namesi
Name:nrdJImported
OrganismiThermotoga maritimaImported
Taxonomic identifieri2336 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi134 ↔ 333Combined sources

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XJEX-ray1.90A/B1-644[»]
1XJFX-ray2.40A/B1-644[»]
1XJGX-ray2.50A/B1-644[»]
1XJJX-ray1.86A/B1-644[»]
1XJKX-ray2.12A/B1-644[»]
1XJMX-ray2.40A/B1-644[»]
1XJNX-ray2.25A/B/C/D1-644[»]
3O0NX-ray1.95A/B1-644[»]
3O0OX-ray1.90A/B1-644[»]
3O0QX-ray1.80A/B1-644[»]
ProteinModelPortaliO33839.
SMRiO33839.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33839.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 99Ribonuc_red_lgNInterPro annotationAdd BLAST85
Domaini132 – 628RIBORED_LARGEInterPro annotationAdd BLAST497

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase class-2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
KOiK00525.

Family and domain databases

CDDicd02888. RNR_II_dimer. 1 hit.
InterProiView protein in InterPro
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013344. RNR_NrdJ/NrdZ.
IPR008926. RNR_R1-su_N.
PfamiView protein in Pfam
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

O33839-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSDLISRW IDVEPSKNAQ IILRDRYFMK DLDGNYLETK WEDVARRVAR
60 70 80 90 100
VVATAELLNP SYKKNEKLDR IKEWEDIFFR VLKARLFIPN SPTLFNAGLG
110 120 130 140 150
VKHDLLWKPI DQMTLEDYEE IYRSRNHLHM LSACFVVPVG DSIEEIFEAV
160 170 180 190 200
KEYALITKVG GGVGSNFSEL RPKGSFVAGT HGKASGPVSF MHVFNSAISV
210 220 230 240 250
VKQGYRRRGA LMGILNINHP DIEEFIDAKK ENTGEAVLNF FNLSVGFPMD
260 270 280 290 300
KKEILKLYEE DGELELSHPR STIRKKVKIR ELFRKIATNA WKSGDPGLAF
310 320 330 340 350
LGEMNKYYPL YPHRKINSTN PCGEIGLSDY EACNLGSIDV AKFYNNGFVD
360 370 380 390 400
LEALQELVQI AVRFLDNVID VNVFPIDKIT KAVKESRRLG LGIMGFADLL
410 420 430 440 450
YKLEIPYNSQ EARDFAANLM AFIALHAHRT SYELGKEKGN FPLLEISRYR
460 470 480 490 500
TEDNFVPFAM GMSNYDDEIR EVMKMTKEFR RNVALLTIAP TGSISNIADT
510 520 530 540 550
SSGLEPNFLL AYTRFVTKED GTKEPLLYVN QVLREKLNPE ILKRIEKELI
560 570 580 590 600
EKGSLKDIPD VPEKIKKVFV VALDIDPMDH LLMQDAFQRY VDNNISKTIN
610 620 630 640 650
MPQSATVDDV LNVYLEALRT NVRGITVYRD GSLQTQVLTK ALKTPEAPKV
660 670 680 690 700
QFFVVDEKLK LHPRPRKDTL RSVTRKYKRP DGTTYITISF DDTGEAVEIF
710 720 730 740 750
ISNGSEMAEA IGRLSSIALR AGVSIDEIVE QLSKVKGEYC KGLAEEIKKA
760 770 780 790 800
LEDFAKLWLR TGEEAPESEE EPIEREKFIV AHNLRWQSGY YVDDEGNVYC
810 820
PVCLSKNSLI KQEGCVSCKN CGWSKCE
Length:827
Mass (Da):94,018
Last modified:January 1, 1998 - v1
Checksum:iD1B99C7F5DBB4256
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12877 Genomic DNA. Translation: CAA73377.1.
PIRiF72414.
RefSeqiNP_227934.1. NC_000853.1.
WP_004082700.1. NZ_CP011108.1.

Genome annotation databases

GeneIDi896945.
KEGGitma:TM0118.
tmq:THMB_0114.
tmx:THMC_0114.
PATRICifig|2336.4.peg.118.

Similar proteinsi

Entry informationi

Entry nameiO33839_THEMT
AccessioniPrimary (citable) accession number: O33839
Entry historyiIntegrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: September 27, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources