Reviewed, UniProtKB/Swiss-Prot O33822 (AAT_THEAQ)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase
      Short name=AspAT
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:

aspC

Organism

Thermus aquaticus

Taxonomic identifier

271 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: InterPro L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Aspartate aminotransferase		PRO_0000123855
Amino acid modifications
	Modified residue	234	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	18	1	A → V in AAB81842. Ref.2
	Sequence conflict	47	1	E → H in AAB81842. Ref.2
	Sequence conflict	52 – 53	2	AG → RA in AAB81842. Ref.2
	Sequence conflict	56	1	A → G in AAB81842. Ref.2
	Sequence conflict	83	1	R → G in AAB81842. Ref.2
	Sequence conflict	186 – 187	2	LR → CE in AAB81842. Ref.2
	Sequence conflict	219 – 220	2	GT → RH in AAB81842. Ref.2
	Sequence conflict	230 – 231	2	NG → ER in AAB81842. Ref.2
	Sequence conflict	234	1	K → N in AAB81842. Ref.2
	Sequence conflict	353	1	E → D in AAB81842. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O33822-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5DD8E2769AA7835B	FASTA	383	41,717
	10 20 30 40 50 60 MRGLSQRVKS MKPSATVAVN ARALELRRKG VDLVALTAGE PDFDTPEHVK EAGRRALAQG 70 80 90 100 110 120 KTKYAPPAGI PELREAVAEK FRRENGLEVT PEETIVTVGG KQALFNLFQA ILDPGDEVIV 130 140 150 160 170 180 LAPYWVSYPE MVRFAGGVPV EVPTLPEEGF VPDPERVRRA ITPRTKALVV NSPNNPTGVV 190 200 210 220 230 240 YPEEVLRALA EMALQHDFYL VSDEIYEHLI YEGAHFSPGT LAPEHTITVN GAAKAFAMTG 250 260 270 280 290 300 WRIGYACGPK AVIKAMADVS SQSTTSPDTI AQWATLEALT NREASMAFIA MAREAYRKRR 310 320 330 340 350 360 DLLLEGLSRI GLEAVRPSGA FYVLMDTSPF APNEVEAAER LLMAGVAVVP GTEFAAFGHV 370 380 RLSYATGEEN LKKALERFAQ ALQ

References

[1]	"Cloning and sequencing of aspartate aminotransferase from Thermus aquaticus YT1." O'Farrell P., Sannia G., Walker J.M., Doonan S. Biochem. Biophys. Res. Commun. 239:810-815(1997) [PubMed: 9367851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YT1.
[2]	Benner E., Ramaley R.F. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YT1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99521 Genomic DNA. Translation: CAA67877.1. AF025665 Genomic DNA. Translation: AAB81842.1.
PIR	JC5775.
3D structure databases
SMR	O33822. Positions 1-382.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.6.1.1. 118.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_THEAQ

Accession

Primary (citable) accession number: O33822
Secondary accession number(s): O31028

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	September 22, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)