4-hydroxybenzoyl-CoA reductase subunit gamma - Thauera aromatica

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Reviewed, UniProtKB/Swiss-Prot O33818 (HCRC_THAAR)

Last modified May 5, 2009. Version 63. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
4-hydroxybenzoyl-CoA reductase subunit gamma
EC=1.3.99.20

Gene names

Name:

hcrC

Organism

Thauera aromatica

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Thauera

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes reductive dehydroxylation of 4-hydroxybenzoyl-CoA. Reaction is not reversible.
Catalytic activity	Benzoyl-CoA + acceptor = 4-hydroxybenzoyl-CoA + reduced acceptor.
Cofactor	Binds 2 2Fe-2S clusters. FAD. Molybdenum.
Enzyme regulation	Inactivated by low concentrations of cyanide.
Subunit structure	Heterohexamer of two alpha, two beta and two gamma subunits.
Sequence similarities	Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxybenzoyl-CoA reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

161

4-hydroxybenzoyl-CoA reductase subunit gamma

PRO_0000083927

Regions

Domain

77

2Fe-2S ferredoxin-type

Sites

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

1

Iron-sulfur (2Fe-2S) Potential

Secondary structure

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161

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

O33818-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: CC2D8172F8EBA03B
Show »

161

17,158

        10         20         30         40         50         60 
MKNILRLTLN GRAREDLVPD NMLLLDYLRE TVGLTGTKQG CDGGECGACT VLVDDRPRLA 

        70         80         90        100        110        120 
CSTLAHQVAG KKVETVESLA TQGTLSKLQA AFHEKLGTQC GFCTPGMIMA SEALLRKNPS 

       130        140        150        160 
PSRDEIKAAL AGNLCRCTGY VRSSKSVETA AAARLCEEGA R

References

[1]	"4-hydroxybenzoyl-CoA reductase (dehydroxylating) from the denitrifying bacterium Thauera aromatica -- prosthetic groups, electron donor, and genes of a member of the molybdenum-flavin-iron-sulfur proteins." Breese K., Fuchs G. Eur. J. Biochem. 251:916-923(1998) [PubMed: 9490068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19. Strain: DSM 6984 / K172.
+	Additional computationally mapped references.

Cross-references

Sequence databases

AJ001830 Genomic DNA. Translation: CAA05037.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RM6	X-ray	1.60	C/F	1-161	[»]
1SB3	X-ray	2.20	C/F	1-161	[»]

ModBase

Enzyme and pathway databases

BRENDA

1.3.99.20. 377.

Family and domain databases

InterPro

IPR002888. 2Fe-2S_bd.
IPR006058. 2Fe2S_fd_BS.
IPR017606. 4hydrxbenzoyl-CoA_Rdtase_gsu.
IPR001041. Ferredoxin.
[Graphical view]

Pfam

PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]

ProDom

PD186071. 2Fe-2S_bind. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR03193. 4hydroxCoAred. 1 hit.

PROSITE

PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

HCRC_THAAR

Accession

Primary (citable) accession number: O33818

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 1, 1998
Last modified:	May 5, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents