SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O33807

- BLC4_SALTM

UniProt

O33807 - BLC4_SALTM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Beta-lactamase CTX-M-4
Gene
bla
Organism
Salmonella typhimurium
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Has cefotaxime-hydrolyzing activity.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase CTX-M-4 (EC:3.5.2.6)
Alternative name(s):
Cefotaximase 4
Gene namesi
Name:bla
Encoded oniPlasmid pMSL0 Publication
OrganismiSalmonella typhimurium
Taxonomic identifieri90371 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi240 – 2401S → A: Causes minor alterations in the interaction of with beta-lactams, reduces slightly the relative hydrolytic activity against cefotaxime and the susceptibility to inhibition by clavulanate. 1 Publication
Mutagenesisi277 – 2771R → N: Confers lower levels of resistance to cefotaxime, ceftriaxone and aztreonam while the levels of resistance to penicillins and penicillin-inhibitor combinations are similar. Slightly less susceptible to inhibition by clavulanate and tazobactam. Cause a 3-fold reduction in the relative rate of hydrolysis of cefotaxime. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 By similarity
Add
BLAST
Chaini29 – 291263Beta-lactamase CTX-M-4
PRO_0000016991Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO33807.
SMRiO33807. Positions 31-291.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 2393Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33807-1 [UniParc]FASTAAdd to Basket

« Hide

MMTQSIRRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV    50
AQINTADNSQ ILYVADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI 100
RASDLVNYNP IAEKHVNGTM TLAELGAGAL QYSDNTAMNK LIAHLGGPDK 150
VTAFARSLGD ETFRLDRTEP TLNSAIPGDP RDTTTPLAMA QTLKNLTLGK 200
ALAETQRAQL VTWLKGNTTG SASIRAGMPK SWGVGDKTGS GDYGTTNDIA 250
VIWPENHAPL VLVTYFTQPE QKAESRRDIL AAAAKIVTHG F 291
Length:291
Mass (Da):31,255
Last modified:January 1, 1998 - v1
Checksum:i2E22E251008DF7C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y14156 Genomic DNA. Translation: CAA74573.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y14156 Genomic DNA. Translation: CAA74573.1 .

3D structure databases

ProteinModelPortali O33807.
SMRi O33807. Positions 31-291.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the gene encoding a plasmid-mediated cefotaxime-hydrolyzing class A beta-lactamase (CTX-M-4): involvement of serine 237 in cephalosporin hydrolysis."
    Gazouli M., Tzelepi E., Sidorenko S.V., Tzouvelekis L.S.
    Antimicrob. Agents Chemother. 42:1259-1262(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF SER-240.
  2. "Effect of substitution of Asn for Arg-276 in the cefotaxime-hydrolyzing class A beta-lactamase CTX-M-4."
    Gazouli M., Legakis N.J., Tzouvelekis L.S.
    FEMS Microbiol. Lett. 169:289-293(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-277.

Entry informationi

Entry nameiBLC4_SALTM
AccessioniPrimary (citable) accession number: O33807
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi