O33807 (BLC4_SALTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase CTX-M-4
EC=3.5.2.6

Alternative name(s):
Cefotaximase 4

Gene names

Name:

bla

Encoded on

Plasmid pMSL

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has cefotaxime-hydrolyzing activity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	By similarity
	Chain	29 – 291	263	Beta-lactamase CTX-M-4		PRO_0000016991
Regions
	Region	237 – 239	3	Substrate binding By similarity
Sites
	Active site	73	1	Acyl-ester intermediate By similarity
Experimental info
	Mutagenesis	240	1	S → A: Causes minor alterations in the interaction of with beta-lactams, reduces slightly the relative hydrolytic activity against cefotaxime and the susceptibility to inhibition by clavulanate. Ref.1
	Mutagenesis	277	1	R → N: Confers lower levels of resistance to cefotaxime, ceftriaxone and aztreonam while the levels of resistance to penicillins and penicillin-inhibitor combinations are similar. Slightly less susceptible to inhibition by clavulanate and tazobactam. Cause a 3-fold reduction in the relative rate of hydrolysis of cefotaxime. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O33807 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2E22E251008DF7C6	FASTA	291	31,255
	10 20 30 40 50 60 MMTQSIRRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV AQINTADNSQ 70 80 90 100 110 120 ILYVADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI RASDLVNYNP IAEKHVNGTM 130 140 150 160 170 180 TLAELGAGAL QYSDNTAMNK LIAHLGGPDK VTAFARSLGD ETFRLDRTEP TLNSAIPGDP 190 200 210 220 230 240 RDTTTPLAMA QTLKNLTLGK ALAETQRAQL VTWLKGNTTG SASIRAGMPK SWGVGDKTGS 250 260 270 280 290 GDYGTTNDIA VIWPENHAPL VLVTYFTQPE QKAESRRDIL AAAAKIVTHG F

References

[1]	"Sequence of the gene encoding a plasmid-mediated cefotaxime-hydrolyzing class A beta-lactamase (CTX-M-4): involvement of serine 237 in cephalosporin hydrolysis." Gazouli M., Tzelepi E., Sidorenko S.V., Tzouvelekis L.S. Antimicrob. Agents Chemother. 42:1259-1262(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF SER-240.
[2]	"Effect of substitution of Asn for Arg-276 in the cefotaxime-hydrolyzing class A beta-lactamase CTX-M-4." Gazouli M., Legakis N.J., Tzouvelekis L.S. FEMS Microbiol. Lett. 169:289-293(1998) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ARG-277.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y14156 Genomic DNA. Translation: CAA74573.1.
3D structure databases
ProteinModelPortal	O33807.
SMR	O33807. Positions 31-291.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLC4_SALTM

Accession

Primary (citable) accession number: O33807

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	March 6, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program