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Protein

Beta-lactamase CTX-M-4

Gene

bla

Organism
Salmonella typhimurium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has cefotaxime-hydrolyzing activity.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Acyl-ester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase CTX-M-4 (EC:3.5.2.6)
Alternative name(s):
Cefotaximase 4
Gene namesi
Name:bla
Encoded oniPlasmid pMSL0 Publication
OrganismiSalmonella typhimurium
Taxonomic identifieri90371 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi240S → A: Causes minor alterations in the interaction of with beta-lactams, reduces slightly the relative hydrolytic activity against cefotaxime and the susceptibility to inhibition by clavulanate. 1 Publication1
Mutagenesisi277R → N: Confers lower levels of resistance to cefotaxime, ceftriaxone and aztreonam while the levels of resistance to penicillins and penicillin-inhibitor combinations are similar. Slightly less susceptible to inhibition by clavulanate and tazobactam. Cause a 3-fold reduction in the relative rate of hydrolysis of cefotaxime. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28By similarityAdd BLAST28
ChainiPRO_000001699129 – 291Beta-lactamase CTX-M-4Add BLAST263

Structurei

3D structure databases

ProteinModelPortaliO33807.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni237 – 239Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK18767.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTQSIRRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV
60 70 80 90 100
AQINTADNSQ ILYVADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI
110 120 130 140 150
RASDLVNYNP IAEKHVNGTM TLAELGAGAL QYSDNTAMNK LIAHLGGPDK
160 170 180 190 200
VTAFARSLGD ETFRLDRTEP TLNSAIPGDP RDTTTPLAMA QTLKNLTLGK
210 220 230 240 250
ALAETQRAQL VTWLKGNTTG SASIRAGMPK SWGVGDKTGS GDYGTTNDIA
260 270 280 290
VIWPENHAPL VLVTYFTQPE QKAESRRDIL AAAAKIVTHG F
Length:291
Mass (Da):31,255
Last modified:January 1, 1998 - v1
Checksum:i2E22E251008DF7C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14156 Genomic DNA. Translation: CAA74573.1.
RefSeqiWP_032489025.1. NG_048990.1.

Genome annotation databases

KEGGiag:CAA74573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14156 Genomic DNA. Translation: CAA74573.1.
RefSeqiWP_032489025.1. NG_048990.1.

3D structure databases

ProteinModelPortaliO33807.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA74573.

Phylogenomic databases

KOiK18767.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLC4_SALTM
AccessioniPrimary (citable) accession number: O33807
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.