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O33807

- BLC4_SALTM

UniProt

O33807 - BLC4_SALTM

Protein

Beta-lactamase CTX-M-4

Gene

bla

Organism
Salmonella typhimurium
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Has cefotaxime-hydrolyzing activity.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase CTX-M-4 (EC:3.5.2.6)
    Alternative name(s):
    Cefotaximase 4
    Gene namesi
    Name:bla
    Encoded oniPlasmid pMSL0 Publication
    OrganismiSalmonella typhimurium
    Taxonomic identifieri90371 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi240 – 2401S → A: Causes minor alterations in the interaction of with beta-lactams, reduces slightly the relative hydrolytic activity against cefotaxime and the susceptibility to inhibition by clavulanate. 1 Publication
    Mutagenesisi277 – 2771R → N: Confers lower levels of resistance to cefotaxime, ceftriaxone and aztreonam while the levels of resistance to penicillins and penicillin-inhibitor combinations are similar. Slightly less susceptible to inhibition by clavulanate and tazobactam. Cause a 3-fold reduction in the relative rate of hydrolysis of cefotaxime. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828By similarityAdd
    BLAST
    Chaini29 – 291263Beta-lactamase CTX-M-4PRO_0000016991Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO33807.
    SMRiO33807. Positions 31-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni237 – 2393Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O33807-1 [UniParc]FASTAAdd to Basket

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    MMTQSIRRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV    50
    AQINTADNSQ ILYVADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI 100
    RASDLVNYNP IAEKHVNGTM TLAELGAGAL QYSDNTAMNK LIAHLGGPDK 150
    VTAFARSLGD ETFRLDRTEP TLNSAIPGDP RDTTTPLAMA QTLKNLTLGK 200
    ALAETQRAQL VTWLKGNTTG SASIRAGMPK SWGVGDKTGS GDYGTTNDIA 250
    VIWPENHAPL VLVTYFTQPE QKAESRRDIL AAAAKIVTHG F 291
    Length:291
    Mass (Da):31,255
    Last modified:January 1, 1998 - v1
    Checksum:i2E22E251008DF7C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14156 Genomic DNA. Translation: CAA74573.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14156 Genomic DNA. Translation: CAA74573.1 .

    3D structure databases

    ProteinModelPortali O33807.
    SMRi O33807. Positions 31-291.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the gene encoding a plasmid-mediated cefotaxime-hydrolyzing class A beta-lactamase (CTX-M-4): involvement of serine 237 in cephalosporin hydrolysis."
      Gazouli M., Tzelepi E., Sidorenko S.V., Tzouvelekis L.S.
      Antimicrob. Agents Chemother. 42:1259-1262(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF SER-240.
    2. "Effect of substitution of Asn for Arg-276 in the cefotaxime-hydrolyzing class A beta-lactamase CTX-M-4."
      Gazouli M., Legakis N.J., Tzouvelekis L.S.
      FEMS Microbiol. Lett. 169:289-293(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-277.

    Entry informationi

    Entry nameiBLC4_SALTM
    AccessioniPrimary (citable) accession number: O33807
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3