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Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Proton acceptorBy similarity
Binding sitei131 – 1311IMPBy similarity
Binding sitei153 – 1531IMP; via carbonyl oxygenBy similarity
Metal bindingi159 – 1591MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 10810IMPBy similarity
Nucleotide bindingi158 – 1592IMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-169-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
Ordered Locus Names:STM0170
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Hypoxanthine phosphoribosyltransferasePRO_0000139635Add
BLAST

Proteomic databases

PaxDbiO33799.
PRIDEiO33799.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi99287.STM0170.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi11 – 3020Combined sources
Beta strandi36 – 416Combined sources
Turni42 – 454Combined sources
Helixi46 – 538Combined sources
Beta strandi61 – 666Combined sources
Beta strandi93 – 10412Combined sources
Helixi106 – 11611Combined sources
Beta strandi121 – 13010Combined sources
Helixi132 – 1343Combined sources
Beta strandi142 – 1476Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi160 – 1623Combined sources
Beta strandi168 – 1747Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J7JX-ray2.30A/B1-178[»]
ProteinModelPortaliO33799.
SMRiO33799. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33799.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.
PhylomeDBiO33799.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSEMVLVG LLRGSFMFMA
60 70 80 90 100
DLCREVQVPH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED
110 120 130 140 150
IIDSGNTLSK VREILGLREP KSLAICTLLD KPSRREVDVP VEFVGFSIPD
160 170
EFVVGYGIDY AQRYRHLPYV GKVVLLDE
Length:178
Mass (Da):20,068
Last modified:August 1, 1998 - v2
Checksum:i5A52E93CAB331357
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008931 Genomic DNA. Translation: AAC46255.1.
AE006468 Genomic DNA. Translation: AAL19134.1.
RefSeqiNP_459175.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19134; AAL19134; STM0170.
GeneIDi1251688.
KEGGistm:STM0170.
PATRICi32378621. VBISalEnt20916_0180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008931 Genomic DNA. Translation: AAC46255.1.
AE006468 Genomic DNA. Translation: AAL19134.1.
RefSeqiNP_459175.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J7JX-ray2.30A/B1-178[»]
ProteinModelPortaliO33799.
SMRiO33799. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0170.

Proteomic databases

PaxDbiO33799.
PRIDEiO33799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19134; AAL19134; STM0170.
GeneIDi1251688.
KEGGistm:STM0170.
PATRICi32378621. VBISalEnt20916_0180.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.
PhylomeDBiO33799.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
BioCyciSENT99287:GCTI-169-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO33799.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A single amino acid substitution in the human and a bacterial hypoxanthine phosphoribosyltransferase modulates specificity for the binding of guanine."
    Lee C.C., Craig S.P. III, Eakin A.E.
    Biochemistry 37:3491-3498(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2 / GP660.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "Crystal structure of the HPRT from Salmonella typhimurium at 2.3 A resolution."
    Lee C.C., Focia P.J., Spraggon G., Eakin A.E.
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

Entry informationi

Entry nameiHPRT_SALTY
AccessioniPrimary (citable) accession number: O33799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.