Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O33799

- HPRT_SALTY

UniProt

O33799 - HPRT_SALTY

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine By similarity.By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Proton acceptorBy similarity
Binding sitei131 – 1311IMPBy similarity
Binding sitei153 – 1531IMP; via carbonyl oxygenBy similarity
Metal bindingi159 – 1591MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 10810IMPBy similarity
Nucleotide bindingi158 – 1592IMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-169-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
Ordered Locus Names:STM0170
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Hypoxanthine phosphoribosyltransferasePRO_0000139635Add
BLAST

Proteomic databases

PaxDbiO33799.
PRIDEiO33799.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi99287.STM0170.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96
Helixi11 – 3020
Beta strandi36 – 416
Turni42 – 454
Helixi46 – 538
Beta strandi61 – 666
Beta strandi93 – 10412
Helixi106 – 11611
Beta strandi121 – 13010
Helixi132 – 1343
Beta strandi142 – 1476
Beta strandi153 – 1553
Beta strandi160 – 1623
Beta strandi168 – 1747

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J7JX-ray2.30A/B1-178[»]
ProteinModelPortaliO33799.
SMRiO33799. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33799.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OMAiVDFMTVS.
OrthoDBiEOG693GNP.
PhylomeDBiO33799.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33799-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSEMVLVG LLRGSFMFMA
60 70 80 90 100
DLCREVQVPH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED
110 120 130 140 150
IIDSGNTLSK VREILGLREP KSLAICTLLD KPSRREVDVP VEFVGFSIPD
160 170
EFVVGYGIDY AQRYRHLPYV GKVVLLDE
Length:178
Mass (Da):20,068
Last modified:August 1, 1998 - v2
Checksum:i5A52E93CAB331357
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008931 Genomic DNA. Translation: AAC46255.1.
AE006468 Genomic DNA. Translation: AAL19134.1.
RefSeqiNP_459175.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19134; AAL19134; STM0170.
GeneIDi1251688.
KEGGistm:STM0170.
PATRICi32378621. VBISalEnt20916_0180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008931 Genomic DNA. Translation: AAC46255.1 .
AE006468 Genomic DNA. Translation: AAL19134.1 .
RefSeqi NP_459175.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J7J X-ray 2.30 A/B 1-178 [» ]
ProteinModelPortali O33799.
SMRi O33799. Positions 1-177.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM0170.

Proteomic databases

PaxDbi O33799.
PRIDEi O33799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL19134 ; AAL19134 ; STM0170 .
GeneIDi 1251688.
KEGGi stm:STM0170.
PATRICi 32378621. VBISalEnt20916_0180.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000236520.
KOi K00760.
OMAi VDFMTVS.
OrthoDBi EOG693GNP.
PhylomeDBi O33799.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .
BioCyci SENT99287:GCTI-169-MONOMER.

Miscellaneous databases

EvolutionaryTracei O33799.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A single amino acid substitution in the human and a bacterial hypoxanthine phosphoribosyltransferase modulates specificity for the binding of guanine."
    Lee C.C., Craig S.P. III, Eakin A.E.
    Biochemistry 37:3491-3498(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2 / GP660.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "Crystal structure of the HPRT from Salmonella typhimurium at 2.3 A resolution."
    Lee C.C., Focia P.J., Spraggon G., Eakin A.E.
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

Entry informationi

Entry nameiHPRT_SALTY
AccessioniPrimary (citable) accession number: O33799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3