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O33799

- HPRT_SALTY

UniProt

O33799 - HPRT_SALTY

Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei103 – 1031Proton acceptorBy similarity
    Binding sitei131 – 1311IMPBy similarity
    Binding sitei153 – 1531IMP; via carbonyl oxygenBy similarity
    Metal bindingi159 – 1591MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi99 – 10810IMPBy similarity
    Nucleotide bindingi158 – 1592IMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. IMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-169-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HPRT
    Gene namesi
    Name:hpt
    Ordered Locus Names:STM0170
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 178178Hypoxanthine phosphoribosyltransferasePRO_0000139635Add
    BLAST

    Proteomic databases

    PaxDbiO33799.
    PRIDEiO33799.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM0170.

    Structurei

    Secondary structure

    1
    178
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 96
    Helixi11 – 3020
    Beta strandi36 – 416
    Turni42 – 454
    Helixi46 – 538
    Beta strandi61 – 666
    Beta strandi93 – 10412
    Helixi106 – 11611
    Beta strandi121 – 13010
    Helixi132 – 1343
    Beta strandi142 – 1476
    Beta strandi153 – 1553
    Beta strandi160 – 1623
    Beta strandi168 – 1747

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J7JX-ray2.30A/B1-178[»]
    ProteinModelPortaliO33799.
    SMRiO33799. Positions 1-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO33799.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000236520.
    KOiK00760.
    OMAiVDFMTVS.
    OrthoDBiEOG693GNP.
    PhylomeDBiO33799.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O33799-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSEMVLVG LLRGSFMFMA    50
    DLCREVQVPH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED 100
    IIDSGNTLSK VREILGLREP KSLAICTLLD KPSRREVDVP VEFVGFSIPD 150
    EFVVGYGIDY AQRYRHLPYV GKVVLLDE 178
    Length:178
    Mass (Da):20,068
    Last modified:August 1, 1998 - v2
    Checksum:i5A52E93CAB331357
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008931 Genomic DNA. Translation: AAC46255.1.
    AE006468 Genomic DNA. Translation: AAL19134.1.
    RefSeqiNP_459175.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL19134; AAL19134; STM0170.
    GeneIDi1251688.
    KEGGistm:STM0170.
    PATRICi32378621. VBISalEnt20916_0180.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008931 Genomic DNA. Translation: AAC46255.1 .
    AE006468 Genomic DNA. Translation: AAL19134.1 .
    RefSeqi NP_459175.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J7J X-ray 2.30 A/B 1-178 [» ]
    ProteinModelPortali O33799.
    SMRi O33799. Positions 1-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM0170.

    Proteomic databases

    PaxDbi O33799.
    PRIDEi O33799.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL19134 ; AAL19134 ; STM0170 .
    GeneIDi 1251688.
    KEGGi stm:STM0170.
    PATRICi 32378621. VBISalEnt20916_0180.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000236520.
    KOi K00760.
    OMAi VDFMTVS.
    OrthoDBi EOG693GNP.
    PhylomeDBi O33799.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci SENT99287:GCTI-169-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O33799.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A single amino acid substitution in the human and a bacterial hypoxanthine phosphoribosyltransferase modulates specificity for the binding of guanine."
      Lee C.C., Craig S.P. III, Eakin A.E.
      Biochemistry 37:3491-3498(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT2 / GP660.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    3. "Crystal structure of the HPRT from Salmonella typhimurium at 2.3 A resolution."
      Lee C.C., Focia P.J., Spraggon G., Eakin A.E.
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

    Entry informationi

    Entry nameiHPRT_SALTY
    AccessioniPrimary (citable) accession number: O33799
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3