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Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine phosphoribosyltransferase (hpt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103Proton acceptorBy similarity1
Binding sitei131IMPBy similarity1
Binding sitei153IMP; via carbonyl oxygenBy similarity1
Metal bindingi159MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi99 – 108IMPBy similarity10
Nucleotide bindingi158 – 159IMPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
Ordered Locus Names:STM0170
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001396351 – 178Hypoxanthine phosphoribosyltransferaseAdd BLAST178

Proteomic databases

PaxDbiO33799.
PRIDEiO33799.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi99287.STM0170.

Structurei

Secondary structure

1178
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi11 – 30Combined sources20
Beta strandi36 – 41Combined sources6
Turni42 – 45Combined sources4
Helixi46 – 53Combined sources8
Beta strandi61 – 66Combined sources6
Beta strandi93 – 104Combined sources12
Helixi106 – 116Combined sources11
Beta strandi121 – 130Combined sources10
Helixi132 – 134Combined sources3
Beta strandi142 – 147Combined sources6
Beta strandi153 – 155Combined sources3
Beta strandi160 – 162Combined sources3
Beta strandi168 – 174Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J7JX-ray2.30A/B1-178[»]
ProteinModelPortaliO33799.
SMRiO33799.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33799.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108UGV. Bacteria.
COG0634. LUCA.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTPVTMDW.
PhylomeDBiO33799.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSEMVLVG LLRGSFMFMA
60 70 80 90 100
DLCREVQVPH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED
110 120 130 140 150
IIDSGNTLSK VREILGLREP KSLAICTLLD KPSRREVDVP VEFVGFSIPD
160 170
EFVVGYGIDY AQRYRHLPYV GKVVLLDE
Length:178
Mass (Da):20,068
Last modified:August 1, 1998 - v2
Checksum:i5A52E93CAB331357
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008931 Genomic DNA. Translation: AAC46255.1.
AE006468 Genomic DNA. Translation: AAL19134.1.
RefSeqiNP_459175.1. NC_003197.1.
WP_000683342.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19134; AAL19134; STM0170.
GeneIDi1251688.
KEGGistm:STM0170.
PATRICi32378621. VBISalEnt20916_0180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008931 Genomic DNA. Translation: AAC46255.1.
AE006468 Genomic DNA. Translation: AAL19134.1.
RefSeqiNP_459175.1. NC_003197.1.
WP_000683342.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J7JX-ray2.30A/B1-178[»]
ProteinModelPortaliO33799.
SMRiO33799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0170.

Proteomic databases

PaxDbiO33799.
PRIDEiO33799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19134; AAL19134; STM0170.
GeneIDi1251688.
KEGGistm:STM0170.
PATRICi32378621. VBISalEnt20916_0180.

Phylogenomic databases

eggNOGiENOG4108UGV. Bacteria.
COG0634. LUCA.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTPVTMDW.
PhylomeDBiO33799.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Miscellaneous databases

EvolutionaryTraceiO33799.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHPRT_SALTY
AccessioniPrimary (citable) accession number: O33799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.