O33777 (HIS5_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisH
EC=2.4.2.-

Alternative name(s):
IGP synthase glutamine amidotransferase subunit
IGP synthase subunit HisH
ImGP synthase subunit HisH
Short name=IGPS subunit HisH

Gene names

Name:	hisH
Ordered Locus Names:	SSO0600
ORF Names:	C08_050

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR By similarity. HAMAP MF_00278
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP MF_00278
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP MF_00278
Subunit structure	Heterodimer of HisH and HisF By similarity. HAMAP MF_00278
Subcellular location	Cytoplasm By similarity HAMAP MF_00278.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Imidazole glycerol phosphate synthase subunit HisH HAMAP MF_00278		PRO_0000152469
Regions
	Domain	2 – 199	198	Glutamine amidotransferase type-1
Sites
	Active site	76	1	Nucleophile By similarity
	Active site	178	1	By similarity
	Active site	180	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33777 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 17DB1A86724CE095	FASTA	199	22,533
	10 20 30 40 50 60 MKALVINYGV GNLYSISSAL KRVGFEVTID NKPRNDYDLI VFPGVGAFSA VAEFILRYRE 70 80 90 100 110 120 LFNDLRRSGT NFLGVCLGMQ IMFEKGTEGK ESNGLGWFKG IVDKINANVK LPHIGWDLVF 130 140 150 160 170 180 EVKDSCELTY GLDKKYVYYV HSYVAYPTSG DYVYMKSQYG IEYPALVCDK NVVGTQFHPE 190 KSSNTGKIFL ENLKGWIKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2." Charlebois R.L., Sensen C.W., Doolittle W.F., Brown J.R. J. Bacteriol. 179:4429-4432(1997) [PubMed: 9209067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2." Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W. Genome 43:116-136(2000) [PubMed: 10701121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82227 Genomic DNA. Translation: AAB63025.1. Y18930 Genomic DNA. Translation: CAB57700.1. AE006641 Genomic DNA. Translation: AAK40911.1.
PIR	H90206.
RefSeq	NP_342121.1. NC_002754.1.
3D structure databases
ProteinModelPortal	O33777.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1454878.
GenomeReviews	Gene locus SSO0600 in contig AE006641_GR.
KEGG	sso:SSO0600.
NMPDR	fig|273057.1.peg.544.
Phylogenomic databases
HOGENOM	HBG292341.
OMA	GAYDQGM.
ProtClustDB	PRK13143.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0600-MONOMER.
Family and domain databases
HAMAP	MF_00278. HisH. [Tree]
InterPro	IPR017926. GATASE_1. IPR010139. Imidazole-glycPsynth_HisH. [Graphical view]
KO	K02501.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PIRSF	PIRSF000495. Amidotransf_hisH. 1 hit.
TIGRFAMs	TIGR01855. IMP_synth_hisH. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS5_SULSO

Accession

Primary (citable) accession number: O33777

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program