O33774 (HIS6_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisF
EC=4.1.3.-

Alternative name(s):
IGP synthase cyclase subunit
IGP synthase subunit HisF
ImGP synthase subunit HisF
Short name=IGPS subunit HisF

Gene names

Name:	hisF
Ordered Locus Names:	SSO0597
ORF Names:	C08_052

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit By similarity. HAMAP MF_01013
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP MF_01013
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP MF_01013
Subunit structure	Heterodimer of HisH and HisF By similarity. HAMAP MF_01013
Subcellular location	Cytoplasm By similarity HAMAP MF_01013.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazoleglycerol-phosphate synthase activity Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Imidazole glycerol phosphate synthase subunit HisF HAMAP MF_01013		PRO_0000142290
Sites
	Active site	11	1	Potential
	Active site	130	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O33774 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 46246934AC1BA850	FASTA	251	26,965
	10 20 30 40 50 60 MTTKRIIACL DVKDGNVVKG VNFLNLQLKG DPVSLASLYE EEGADEIVFL DITATIEARK 70 80 90 100 110 120 ALYNVIKDTA SVLSIPLTVG GGIRTPDDVS MALRSGADKV SINTAAVESS QIVKKSAEEF 130 140 150 160 170 180 GSQAVVVAID VKKVSGNWIV FTKSGTYNTR LDAIKWAKKV EELGAGEILL TSIDRDGTRL 190 200 210 220 230 240 GYDLELTRKI VDSVNIPVIA SGGAGKMEHF YEVFSLAKAD AALAAGIFHD GIIKIKDLKS 250 YLSQKGIEVR M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2." Charlebois R.L., Sensen C.W., Doolittle W.F., Brown J.R. J. Bacteriol. 179:4429-4432(1997) [PubMed: 9209067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2." Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W. Genome 43:116-136(2000) [PubMed: 10701121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82227 Genomic DNA. Translation: AAB63022.1. Y18930 Genomic DNA. Translation: CAB57702.1. AE006641 Genomic DNA. Translation: AAK40908.1.
PIR	E90206.
RefSeq	NP_342118.1. NC_002754.1.
3D structure databases
ProteinModelPortal	O33774.
SMR	O33774. Positions 1-251.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1454875.
GenomeReviews	Gene locus SSO0597 in contig AE006641_GR.
KEGG	sso:SSO0597.
NMPDR	fig|273057.1.peg.541.
Phylogenomic databases
HOGENOM	HBG541613.
OMA	RVVKGTN.
ProtClustDB	PRK02083.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0597-MONOMER.
Family and domain databases
HAMAP	MF_01013. HisF. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR004651. HisF. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K02500.
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00735. HisF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS6_SULSO

Accession

Primary (citable) accession number: O33774

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program