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O33768 (SYL2_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase 2

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase 2
Short name=LeuRS 2
Gene names
Name:leuS2
Synonyms:leuS
Ordered Locus Names:SSO0589
ORF Names:C21_009
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length944 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 944944Leucine--tRNA ligase 2 HAMAP-Rule MF_00049
PRO_0000152145

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif623 – 6275"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6261ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O33768 [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: 9ACAFCFBF04160DC

FASTA944109,104
        10         20         30         40         50         60 
MNNIAYKWQT RWEEDKIYES NPNPSKPKFF TTVAFPYPNS PWHIGHGRTY VTGDILARYK 

        70         80         90        100        110        120 
RMRGYNVLFP MAFHYTGTPI MAMADAIAKG DKELIETFKD IYEISPDVIP RMSDPLFMAN 

       130        140        150        160        170        180 
YFKEDIKASM REIGLGIDWR REFTTIDPEF SSFVTWQFHK LQSKGYIVKD THPVGWCPVH 

       190        200        210        220        230        240 
HIPVGMHDTK GDVEPEIGEF VLIYFNSEKG IFPAATLRPE TIFGATALWI NPSEMYVVAS 

       250        260        270        280        290        300 
MLGKKMILSE KAAAKLSFQI DDIEIEEKIK GSKLVGLKVE NPITGKHIAV LGADFVDVSL 

       310        320        330        340        350        360 
GTGVVMSVPA HAPFDYYYSK KTFKNNNIEI IPVITVEGLG NALAKDVVEK NNPKSDEDLK 

       370        380        390        400        410        420 
KLTEYVYRTE YNKGVLRSDL GNLIREEYRN ELKSLGGLPV PKGRELITNF LISKGLGRKI 

       430        440        450        460        470        480 
FEVMNKPVYC RCGTEIVVKI LKDQWFLDYS NKEWKELARK SLSKINVIPE ESRKDFEFTI 

       490        500        510        520        530        540 
EWLEKRACAR TRGLGTPLPW DKKWIIESLS DSTIYMAYYT ISHKIKQYKL SPSKLTQEFW 

       550        560        570        580        590        600 
DYVMLGIGNL EEISEKTGIP SNIIKEFREE FLYWYPLDIR HSGKDLIPNH LTFFIFNHAA 

       610        620        630        640        650        660 
IFQENLWPKA IAVNGLVLYE GKKMSKSLRN IIPLRKGLKM YGVDVMRIAV SSTADMGSDV 

       670        680        690        700        710        720 
NFSESLVKTV GETLRKMYEL FKSLDNYTGD ILGFPEKWLL SRIYEITSST TRHMEALELR 

       730        740        750        760        770        780 
DAVNELLFVF SSDLDEYFGM VSAEGREANN KVLREVLTIW LKLITPFAPH LAEEIWHEIL 

       790        800        810        820        830        840 
KQKTYIVNEG WPEVEGSKMD ELTLLEHEYM KRIVEDIRSI LNIFKGTPKL IKIYALNDSR 

       850        860        870        880        890        900 
YMELLRDAIV ANGQMKKFMD IHKPKSREDA RILQKIFNES LEIDDKMKKL VTNYNINEVD 

       910        920        930        940 
VLNKLSKYIR RKLNVDILIE PYDEEVKKTY NKEAMPLRPA IIIE 

« Hide

References

« Hide 'large scale' references
[1]"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2."
Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. expand/collapse author list , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.
Genome 43:116-136(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]"The complete genome of the crenarchaeon Sulfolobus solfataricus P2."
She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. expand/collapse author list , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.
Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]"Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2."
Charlebois R.L., Sensen C.W., Doolittle W.F., Brown J.R.
J. Bacteriol. 179:4429-4432(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 762-944.
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y18930 Genomic DNA. Translation: CAB57710.1.
AE006641 Genomic DNA. Translation: AAK40902.1.
U82227 Genomic DNA. Translation: AAB63016.1.
PIRG90205.
RefSeqNP_342112.1. NC_002754.1.

3D structure databases

ProteinModelPortalO33768.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273057.SSO0589.

Proteomic databases

PRIDEO33768.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK40902; AAK40902; SSO0589.
GeneID1454869.
KEGGsso:SSO0589.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAGFYEGVM.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycSSOL273057:GCH2-564-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL2_SULSO
AccessionPrimary (citable) accession number: O33768
Secondary accession number(s): Q9UWZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 20, 2001
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries