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O33732

- NARB_SHEFN

UniProt

O33732 - NARB_SHEFN

Protein

Nitrate reductase

Gene

Sfri_1505

Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.Curated
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi11 – 111Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi15 – 151Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. molybdenum ion binding Source: InterPro
    3. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrate assimilation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrate assimilation

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciSFRI318167:GIXS-1564-MONOMER.
    UniPathwayiUPA00653.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrate reductase (EC:1.7.99.4)
    Gene namesi
    Ordered Locus Names:Sfri_1505
    OrganismiShewanella frigidimarina (strain NCIMB 400)
    Taxonomic identifieri318167 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000000684: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Nitrate reductasePRO_0000063236Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi318167.Sfri_1505.

    Structurei

    3D structure databases

    ProteinModelPortaliO33732.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 64644Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031440.
    KOiK00372.
    OMAiLGCGTQC.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR007419. BFD-like_2Fe2S-bd_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view]
    PfamiPF04324. Fer2_BFD. 1 hit.
    PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O33732-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVVQSSCAY CGVGCGVSVS SNKPNWTDVD AADLILVGDN KHPANYGHLC    50
    AKGERLLDSL AQPNVLRYPK LRSGMPLDWD KASTLIADTF AKTIAEHGPD 100
    SVALYLSGQL LTEDYYVANK FAKGFLKTAN VDTNSRLCMS SAVSAMQRAF 150
    GEDVVPGCYD DLEQADVIVL VGANTAWTHP VLFQRILAAI KANNAQLVVI 200
    DPLSTATAKQ ADLHLAIKPG ADLTLFHGLL GYLADQNRVD HAYIAAHTEG 250
    FDTVVLQAQQ LSANLADLAT QVGVSVTQLT QFYQLVANNK KVLTASCQGV 300
    NQSTIGTDAT NAMINCHLAL GHIGQAGCGF FSLTGQPNAM GGREVGGLAT 350
    QLACHMGFSQ PEQQLLADFW KVDSIADQKG LVAVEMFDAL AEGKIKAIWI 400
    MGTNPVVSLP NSEKIAQALA DCPFVVVSEI SPDSDTAKLA DVLLPAQGWS 450
    EKCGTVTNSE RTITRQRGFI TAKGQAKPDW WAVSQVAKKM GFDGFEFDDN 500
    ASIFSEFAAL SAKVKQVFPT KVFDLTGLTE LSKAQYDALA PTQWPIASAT 550
    QIGQQNVRVF GLGEFATATG KAQFVTPAVV SVPQQSLPSN TLLLNTGRSR 600
    DQWHTMTRTG HIASLRASIP EPVVHLHSSQ LSALSLTEGG LVRIEAIQNQ 650
    IEQYSTETAN PDLFTHASFT MARAVVDDDI PTNMALMSMH WSAQFSLTKG 700
    VNQALDARVD PISKQPGFKC QPVTLTPVEL ALQGVVFGQH YSSAHGLCWQ 750
    VAQTLENGVC HHIGFTDTDD GFAYQATVHS LKWTLTVVGQ PLYIQCNMDK 800
    GLLKALKVLS HTQVNVALYQ MNDFIGKPVD KQLIKQLHQQ IKAGNSPLIC 850
    ACTGVTEANI NDEINQQFND QVMSDGLANI SFEQALDSTQ LLLGCGRQCG 900
    SCHSEVKQCA KQSWKDALSY CESYSDIDHQ PSVAEDVA 938
    Length:938
    Mass (Da):101,087
    Last modified:December 12, 2006 - v2
    Checksum:iC8951CAC0306A663
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000447 Genomic DNA. Translation: ABI71356.1.
    AJ000006 Genomic DNA. Translation: CAA03851.1.
    RefSeqiYP_750194.1. NC_008345.1.

    Genome annotation databases

    EnsemblBacteriaiABI71356; ABI71356; Sfri_1505.
    GeneIDi4277460.
    KEGGisfr:Sfri_1505.
    PATRICi23496939. VBISheFri14343_1562.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000447 Genomic DNA. Translation: ABI71356.1 .
    AJ000006 Genomic DNA. Translation: CAA03851.1 .
    RefSeqi YP_750194.1. NC_008345.1.

    3D structure databases

    ProteinModelPortali O33732.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 318167.Sfri_1505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI71356 ; ABI71356 ; Sfri_1505 .
    GeneIDi 4277460.
    KEGGi sfr:Sfri_1505.
    PATRICi 23496939. VBISheFri14343_1562.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031440.
    KOi K00372.
    OMAi LGCGTQC.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    UniPathwayi UPA00653 .
    BioCyci SFRI318167:GIXS-1564-MONOMER.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR007419. BFD-like_2Fe2S-bd_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view ]
    Pfami PF04324. Fer2_BFD. 1 hit.
    PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCIMB 400.
    2. "Identification and characterization of a novel cytochrome c3 from Shewanella frigidimarina that is involved in Fe(III) respiration."
      Gordon E.H.J., Pike A.D., Hill A.E., Cuthbertson P.M., Chapman S.K., Reid G.A.
      Biochem. J. 349:153-158(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-397.

    Entry informationi

    Entry nameiNARB_SHEFN
    AccessioniPrimary (citable) accession number: O33732
    Secondary accession number(s): Q084F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3