O33732 (NARB_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrate reductase
EC=1.7.99.4

Gene names

Ordered Locus Names:

Sfri_1505

Organism

Shewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]

Taxonomic identifier

318167 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella frigidimarina

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential. Molybdenum (molybdopterin) By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Nitrate assimilation
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 938	938	Nitrate reductase		PRO_0000063236
Sites
	Metal binding	8	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	11	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	15	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33732 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: C8951CAC0306A663	FASTA	938	101,087
	10 20 30 40 50 60 MSVVQSSCAY CGVGCGVSVS SNKPNWTDVD AADLILVGDN KHPANYGHLC AKGERLLDSL 70 80 90 100 110 120 AQPNVLRYPK LRSGMPLDWD KASTLIADTF AKTIAEHGPD SVALYLSGQL LTEDYYVANK 130 140 150 160 170 180 FAKGFLKTAN VDTNSRLCMS SAVSAMQRAF GEDVVPGCYD DLEQADVIVL VGANTAWTHP 190 200 210 220 230 240 VLFQRILAAI KANNAQLVVI DPLSTATAKQ ADLHLAIKPG ADLTLFHGLL GYLADQNRVD 250 260 270 280 290 300 HAYIAAHTEG FDTVVLQAQQ LSANLADLAT QVGVSVTQLT QFYQLVANNK KVLTASCQGV 310 320 330 340 350 360 NQSTIGTDAT NAMINCHLAL GHIGQAGCGF FSLTGQPNAM GGREVGGLAT QLACHMGFSQ 370 380 390 400 410 420 PEQQLLADFW KVDSIADQKG LVAVEMFDAL AEGKIKAIWI MGTNPVVSLP NSEKIAQALA 430 440 450 460 470 480 DCPFVVVSEI SPDSDTAKLA DVLLPAQGWS EKCGTVTNSE RTITRQRGFI TAKGQAKPDW 490 500 510 520 530 540 WAVSQVAKKM GFDGFEFDDN ASIFSEFAAL SAKVKQVFPT KVFDLTGLTE LSKAQYDALA 550 560 570 580 590 600 PTQWPIASAT QIGQQNVRVF GLGEFATATG KAQFVTPAVV SVPQQSLPSN TLLLNTGRSR 610 620 630 640 650 660 DQWHTMTRTG HIASLRASIP EPVVHLHSSQ LSALSLTEGG LVRIEAIQNQ IEQYSTETAN 670 680 690 700 710 720 PDLFTHASFT MARAVVDDDI PTNMALMSMH WSAQFSLTKG VNQALDARVD PISKQPGFKC 730 740 750 760 770 780 QPVTLTPVEL ALQGVVFGQH YSSAHGLCWQ VAQTLENGVC HHIGFTDTDD GFAYQATVHS 790 800 810 820 830 840 LKWTLTVVGQ PLYIQCNMDK GLLKALKVLS HTQVNVALYQ MNDFIGKPVD KQLIKQLHQQ 850 860 870 880 890 900 IKAGNSPLIC ACTGVTEANI NDEINQQFND QVMSDGLANI SFEQALDSTQ LLLGCGRQCG 910 920 930 SCHSEVKQCA KQSWKDALSY CESYSDIDHQ PSVAEDVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of Shewanella frigidimarina NCIMB 400." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCIMB 400.
[2]	"Identification and characterization of a novel cytochrome c3 from Shewanella frigidimarina that is involved in Fe(III) respiration." Gordon E.H.J., Pike A.D., Hill A.E., Cuthbertson P.M., Chapman S.K., Reid G.A. Biochem. J. 349:153-158(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-397.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000447 Genomic DNA. Translation: ABI71356.1. AJ000006 Genomic DNA. Translation: CAA03851.1.
RefSeq	YP_750194.1. NC_008345.1.
3D structure databases
ProteinModelPortal	O33732.
ModBase	Search...
Protein-protein interaction databases
STRING	318167.Sfri_1505.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI71356; ABI71356; Sfri_1505.
GeneID	4277460.
KEGG	sfr:Sfri_1505.
PATRIC	23496939. VBISheFri14343_1562.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031440.
OMA	FENDGQR.
ProtClustDB	CLSK958261.
Enzyme and pathway databases
BioCyc	SFRI318167:GIXS-1564-MONOMER.
UniPathway	UPA00653.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR007419. BFD-like_2Fe2S-bd_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. [Graphical view]
Pfam	PF04324. Fer2_BFD. 1 hit. PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NARB_SHEFN

Accession

Primary (citable) accession number: O33732
Secondary accession number(s): Q084F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	December 12, 2006
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program