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O33732

- NARB_SHEFN

UniProt

O33732 - NARB_SHEFN

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Protein

Nitrate reductase

Gene
Sfri_1505
Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 1 4Fe-4S cluster Reviewed prediction.
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Iron-sulfur (4Fe-4S) By similarity
Metal bindingi11 – 111Iron-sulfur (4Fe-4S) By similarity
Metal bindingi15 – 151Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. molybdenum ion binding Source: InterPro
  3. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSFRI318167:GIXS-1564-MONOMER.
UniPathwayiUPA00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase (EC:1.7.99.4)
Gene namesi
Ordered Locus Names:Sfri_1505
OrganismiShewanella frigidimarina (strain NCIMB 400)
Taxonomic identifieri318167 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000684: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Nitrate reductasePRO_0000063236Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi318167.Sfri_1505.

Structurei

3D structure databases

ProteinModelPortaliO33732.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 64644Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031440.
KOiK00372.
OMAiLGCGTQC.
OrthoDBiEOG6CVV7G.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04324. Fer2_BFD. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33732-1 [UniParc]FASTAAdd to Basket

« Hide

MSVVQSSCAY CGVGCGVSVS SNKPNWTDVD AADLILVGDN KHPANYGHLC    50
AKGERLLDSL AQPNVLRYPK LRSGMPLDWD KASTLIADTF AKTIAEHGPD 100
SVALYLSGQL LTEDYYVANK FAKGFLKTAN VDTNSRLCMS SAVSAMQRAF 150
GEDVVPGCYD DLEQADVIVL VGANTAWTHP VLFQRILAAI KANNAQLVVI 200
DPLSTATAKQ ADLHLAIKPG ADLTLFHGLL GYLADQNRVD HAYIAAHTEG 250
FDTVVLQAQQ LSANLADLAT QVGVSVTQLT QFYQLVANNK KVLTASCQGV 300
NQSTIGTDAT NAMINCHLAL GHIGQAGCGF FSLTGQPNAM GGREVGGLAT 350
QLACHMGFSQ PEQQLLADFW KVDSIADQKG LVAVEMFDAL AEGKIKAIWI 400
MGTNPVVSLP NSEKIAQALA DCPFVVVSEI SPDSDTAKLA DVLLPAQGWS 450
EKCGTVTNSE RTITRQRGFI TAKGQAKPDW WAVSQVAKKM GFDGFEFDDN 500
ASIFSEFAAL SAKVKQVFPT KVFDLTGLTE LSKAQYDALA PTQWPIASAT 550
QIGQQNVRVF GLGEFATATG KAQFVTPAVV SVPQQSLPSN TLLLNTGRSR 600
DQWHTMTRTG HIASLRASIP EPVVHLHSSQ LSALSLTEGG LVRIEAIQNQ 650
IEQYSTETAN PDLFTHASFT MARAVVDDDI PTNMALMSMH WSAQFSLTKG 700
VNQALDARVD PISKQPGFKC QPVTLTPVEL ALQGVVFGQH YSSAHGLCWQ 750
VAQTLENGVC HHIGFTDTDD GFAYQATVHS LKWTLTVVGQ PLYIQCNMDK 800
GLLKALKVLS HTQVNVALYQ MNDFIGKPVD KQLIKQLHQQ IKAGNSPLIC 850
ACTGVTEANI NDEINQQFND QVMSDGLANI SFEQALDSTQ LLLGCGRQCG 900
SCHSEVKQCA KQSWKDALSY CESYSDIDHQ PSVAEDVA 938
Length:938
Mass (Da):101,087
Last modified:December 12, 2006 - v2
Checksum:iC8951CAC0306A663
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000447 Genomic DNA. Translation: ABI71356.1.
AJ000006 Genomic DNA. Translation: CAA03851.1.
RefSeqiYP_750194.1. NC_008345.1.

Genome annotation databases

EnsemblBacteriaiABI71356; ABI71356; Sfri_1505.
GeneIDi4277460.
KEGGisfr:Sfri_1505.
PATRICi23496939. VBISheFri14343_1562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000447 Genomic DNA. Translation: ABI71356.1 .
AJ000006 Genomic DNA. Translation: CAA03851.1 .
RefSeqi YP_750194.1. NC_008345.1.

3D structure databases

ProteinModelPortali O33732.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 318167.Sfri_1505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI71356 ; ABI71356 ; Sfri_1505 .
GeneIDi 4277460.
KEGGi sfr:Sfri_1505.
PATRICi 23496939. VBISheFri14343_1562.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031440.
KOi K00372.
OMAi LGCGTQC.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

UniPathwayi UPA00653 .
BioCyci SFRI318167:GIXS-1564-MONOMER.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view ]
Pfami PF04324. Fer2_BFD. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIMB 400.
  2. "Identification and characterization of a novel cytochrome c3 from Shewanella frigidimarina that is involved in Fe(III) respiration."
    Gordon E.H.J., Pike A.D., Hill A.E., Cuthbertson P.M., Chapman S.K., Reid G.A.
    Biochem. J. 349:153-158(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-397.

Entry informationi

Entry nameiNARB_SHEFN
AccessioniPrimary (citable) accession number: O33732
Secondary accession number(s): Q084F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3