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O33732 (NARB_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrate reductase

EC=1.7.99.4
Gene names
Ordered Locus Names:Sfri_1505
OrganismShewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]
Taxonomic identifier318167 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length938 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 1 4Fe-4S cluster Potential.

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation).

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Ontologies

Keywords
   Biological processNitrate assimilation
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
Molybdenum
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processnitrate assimilation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

molybdenum ion binding

Inferred from electronic annotation. Source: InterPro

nitrate reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 938938Nitrate reductase
PRO_0000063236

Regions

Domain1 – 64644Fe-4S Mo/W bis-MGD-type

Sites

Metal binding81Iron-sulfur (4Fe-4S) By similarity
Metal binding111Iron-sulfur (4Fe-4S) By similarity
Metal binding151Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
O33732 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: C8951CAC0306A663

FASTA938101,087
        10         20         30         40         50         60 
MSVVQSSCAY CGVGCGVSVS SNKPNWTDVD AADLILVGDN KHPANYGHLC AKGERLLDSL 

        70         80         90        100        110        120 
AQPNVLRYPK LRSGMPLDWD KASTLIADTF AKTIAEHGPD SVALYLSGQL LTEDYYVANK 

       130        140        150        160        170        180 
FAKGFLKTAN VDTNSRLCMS SAVSAMQRAF GEDVVPGCYD DLEQADVIVL VGANTAWTHP 

       190        200        210        220        230        240 
VLFQRILAAI KANNAQLVVI DPLSTATAKQ ADLHLAIKPG ADLTLFHGLL GYLADQNRVD 

       250        260        270        280        290        300 
HAYIAAHTEG FDTVVLQAQQ LSANLADLAT QVGVSVTQLT QFYQLVANNK KVLTASCQGV 

       310        320        330        340        350        360 
NQSTIGTDAT NAMINCHLAL GHIGQAGCGF FSLTGQPNAM GGREVGGLAT QLACHMGFSQ 

       370        380        390        400        410        420 
PEQQLLADFW KVDSIADQKG LVAVEMFDAL AEGKIKAIWI MGTNPVVSLP NSEKIAQALA 

       430        440        450        460        470        480 
DCPFVVVSEI SPDSDTAKLA DVLLPAQGWS EKCGTVTNSE RTITRQRGFI TAKGQAKPDW 

       490        500        510        520        530        540 
WAVSQVAKKM GFDGFEFDDN ASIFSEFAAL SAKVKQVFPT KVFDLTGLTE LSKAQYDALA 

       550        560        570        580        590        600 
PTQWPIASAT QIGQQNVRVF GLGEFATATG KAQFVTPAVV SVPQQSLPSN TLLLNTGRSR 

       610        620        630        640        650        660 
DQWHTMTRTG HIASLRASIP EPVVHLHSSQ LSALSLTEGG LVRIEAIQNQ IEQYSTETAN 

       670        680        690        700        710        720 
PDLFTHASFT MARAVVDDDI PTNMALMSMH WSAQFSLTKG VNQALDARVD PISKQPGFKC 

       730        740        750        760        770        780 
QPVTLTPVEL ALQGVVFGQH YSSAHGLCWQ VAQTLENGVC HHIGFTDTDD GFAYQATVHS 

       790        800        810        820        830        840 
LKWTLTVVGQ PLYIQCNMDK GLLKALKVLS HTQVNVALYQ MNDFIGKPVD KQLIKQLHQQ 

       850        860        870        880        890        900 
IKAGNSPLIC ACTGVTEANI NDEINQQFND QVMSDGLANI SFEQALDSTQ LLLGCGRQCG 

       910        920        930 
SCHSEVKQCA KQSWKDALSY CESYSDIDHQ PSVAEDVA 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of Shewanella frigidimarina NCIMB 400."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. expand/collapse author list , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCIMB 400.
[2]"Identification and characterization of a novel cytochrome c3 from Shewanella frigidimarina that is involved in Fe(III) respiration."
Gordon E.H.J., Pike A.D., Hill A.E., Cuthbertson P.M., Chapman S.K., Reid G.A.
Biochem. J. 349:153-158(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-397.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000447 Genomic DNA. Translation: ABI71356.1.
AJ000006 Genomic DNA. Translation: CAA03851.1.
RefSeqYP_750194.1. NC_008345.1.

3D structure databases

ProteinModelPortalO33732.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318167.Sfri_1505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI71356; ABI71356; Sfri_1505.
GeneID4277460.
KEGGsfr:Sfri_1505.
PATRIC23496939. VBISheFri14343_1562.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031440.
KOK00372.
OMALGCGTQC.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycSFRI318167:GIXS-1564-MONOMER.
UniPathwayUPA00653.

Family and domain databases

InterProIPR009010. Asp_de-COase-like_dom.
IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamPF04324. Fer2_BFD. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNARB_SHEFN
AccessionPrimary (citable) accession number: O33732
Secondary accession number(s): Q084F9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways