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O33732

- NARB_SHEFN

UniProt

O33732 - NARB_SHEFN

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Protein

Nitrate reductase

Gene

Sfri_1505

Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 1 4Fe-4S cluster.Curated
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi11 – 111Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi15 – 151Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. molybdenum ion binding Source: InterPro
  3. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSFRI318167:GIXS-1564-MONOMER.
UniPathwayiUPA00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase (EC:1.7.99.4)
Gene namesi
Ordered Locus Names:Sfri_1505
OrganismiShewanella frigidimarina (strain NCIMB 400)
Taxonomic identifieri318167 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000684: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Nitrate reductasePRO_0000063236Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi318167.Sfri_1505.

Structurei

3D structure databases

ProteinModelPortaliO33732.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 64644Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031440.
KOiK00372.
OMAiLGCGTQC.
OrthoDBiEOG6CVV7G.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04324. Fer2_BFD. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33732-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVVQSSCAY CGVGCGVSVS SNKPNWTDVD AADLILVGDN KHPANYGHLC
60 70 80 90 100
AKGERLLDSL AQPNVLRYPK LRSGMPLDWD KASTLIADTF AKTIAEHGPD
110 120 130 140 150
SVALYLSGQL LTEDYYVANK FAKGFLKTAN VDTNSRLCMS SAVSAMQRAF
160 170 180 190 200
GEDVVPGCYD DLEQADVIVL VGANTAWTHP VLFQRILAAI KANNAQLVVI
210 220 230 240 250
DPLSTATAKQ ADLHLAIKPG ADLTLFHGLL GYLADQNRVD HAYIAAHTEG
260 270 280 290 300
FDTVVLQAQQ LSANLADLAT QVGVSVTQLT QFYQLVANNK KVLTASCQGV
310 320 330 340 350
NQSTIGTDAT NAMINCHLAL GHIGQAGCGF FSLTGQPNAM GGREVGGLAT
360 370 380 390 400
QLACHMGFSQ PEQQLLADFW KVDSIADQKG LVAVEMFDAL AEGKIKAIWI
410 420 430 440 450
MGTNPVVSLP NSEKIAQALA DCPFVVVSEI SPDSDTAKLA DVLLPAQGWS
460 470 480 490 500
EKCGTVTNSE RTITRQRGFI TAKGQAKPDW WAVSQVAKKM GFDGFEFDDN
510 520 530 540 550
ASIFSEFAAL SAKVKQVFPT KVFDLTGLTE LSKAQYDALA PTQWPIASAT
560 570 580 590 600
QIGQQNVRVF GLGEFATATG KAQFVTPAVV SVPQQSLPSN TLLLNTGRSR
610 620 630 640 650
DQWHTMTRTG HIASLRASIP EPVVHLHSSQ LSALSLTEGG LVRIEAIQNQ
660 670 680 690 700
IEQYSTETAN PDLFTHASFT MARAVVDDDI PTNMALMSMH WSAQFSLTKG
710 720 730 740 750
VNQALDARVD PISKQPGFKC QPVTLTPVEL ALQGVVFGQH YSSAHGLCWQ
760 770 780 790 800
VAQTLENGVC HHIGFTDTDD GFAYQATVHS LKWTLTVVGQ PLYIQCNMDK
810 820 830 840 850
GLLKALKVLS HTQVNVALYQ MNDFIGKPVD KQLIKQLHQQ IKAGNSPLIC
860 870 880 890 900
ACTGVTEANI NDEINQQFND QVMSDGLANI SFEQALDSTQ LLLGCGRQCG
910 920 930
SCHSEVKQCA KQSWKDALSY CESYSDIDHQ PSVAEDVA
Length:938
Mass (Da):101,087
Last modified:December 12, 2006 - v2
Checksum:iC8951CAC0306A663
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000447 Genomic DNA. Translation: ABI71356.1.
AJ000006 Genomic DNA. Translation: CAA03851.1.
RefSeqiYP_750194.1. NC_008345.1.

Genome annotation databases

EnsemblBacteriaiABI71356; ABI71356; Sfri_1505.
GeneIDi4277460.
KEGGisfr:Sfri_1505.
PATRICi23496939. VBISheFri14343_1562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000447 Genomic DNA. Translation: ABI71356.1 .
AJ000006 Genomic DNA. Translation: CAA03851.1 .
RefSeqi YP_750194.1. NC_008345.1.

3D structure databases

ProteinModelPortali O33732.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 318167.Sfri_1505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI71356 ; ABI71356 ; Sfri_1505 .
GeneIDi 4277460.
KEGGi sfr:Sfri_1505.
PATRICi 23496939. VBISheFri14343_1562.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031440.
KOi K00372.
OMAi LGCGTQC.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

UniPathwayi UPA00653 .
BioCyci SFRI318167:GIXS-1564-MONOMER.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view ]
Pfami PF04324. Fer2_BFD. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIMB 400.
  2. "Identification and characterization of a novel cytochrome c3 from Shewanella frigidimarina that is involved in Fe(III) respiration."
    Gordon E.H.J., Pike A.D., Hill A.E., Cuthbertson P.M., Chapman S.K., Reid G.A.
    Biochem. J. 349:153-158(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-397.

Entry informationi

Entry nameiNARB_SHEFN
AccessioniPrimary (citable) accession number: O33732
Secondary accession number(s): Q084F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: December 12, 2006
Last modified: October 29, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3