Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kexstatin-1

Gene
N/A
Organism
Streptomyces platensis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Strongly inhibits the kexin (KEX2) protease. Also active on subtilisin but not thermolysin, trypsin, or chymotrypsin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei105 – 1062Reactive bond

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI16.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Kexstatin-1
Alternative name(s):
Kexstatin I
OrganismiStreptomyces platensis
Taxonomic identifieri58346 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Propeptidei26 – 3510PRO_0000033276
Chaini36 – 145110Kexstatin-1PRO_0000033277Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi66 ↔ 81By similarity
Disulfide bondi103 ↔ 133By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliO33702.
SMRiO33702. Positions 38-145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.350.10. 1 hit.
HAMAPiMF_00778. SSI.
InterProiIPR000691. Prot_inh_I16_SSI.
IPR020054. Prot_inh_SSI_I16_CS.
IPR023549. Subtilisin_inhibitor.
[Graphical view]
PfamiPF00720. SSI. 1 hit.
[Graphical view]
PRINTSiPR00294. SSBTLNINHBTR.
ProDomiPD004028. Prot_inh_SSI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55399. SSF55399. 1 hit.
PROSITEiPS00999. SSI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYITGAVAL GAALVLGTLA TTAQAAAPAQ PARTGGLYAP TELVLTVGQG
60 70 80 90 100
ESRATATVQR AVTLSCMPGA RGSHPNPLGA CTQLRAVAGD FNAITAATSD
110 120 130 140
RLCTKEWNPL VVTADGVWQG KRVSYSYTFA NRCEMNIDSD TVFNF
Length:145
Mass (Da):15,125
Last modified:January 1, 1998 - v1
Checksum:i80F74226735FE0DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005264 Genomic DNA. Translation: BAA21112.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005264 Genomic DNA. Translation: BAA21112.1.

3D structure databases

ProteinModelPortaliO33702.
SMRiO33702. Positions 38-145.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI16.002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.350.10. 1 hit.
HAMAPiMF_00778. SSI.
InterProiIPR000691. Prot_inh_I16_SSI.
IPR020054. Prot_inh_SSI_I16_CS.
IPR023549. Subtilisin_inhibitor.
[Graphical view]
PfamiPF00720. SSI. 1 hit.
[Graphical view]
PRINTSiPR00294. SSBTLNINHBTR.
ProDomiPD004028. Prot_inh_SSI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55399. SSF55399. 1 hit.
PROSITEiPS00999. SSI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and rational mutagenesis of kexstatin I, a potent proteinaceous inhibitor of Kex2 proteinase."
    Oda K., Oyama H., Ito S., Fukiharu M., Miyagawa Y., Takahashi S., Hirose M., Kikuchi N., Nakayama T., Shibano Y.
    Biochem. J. 355:339-346(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS OF THR-104 AND LYS-105.
    Strain: Q268.
  2. "A novel proteinaceous Kex 2 proteinase inhibitor, kexstatin, from Streptomyces platensis Q268."
    Oda K., Takahashi S., Kikuchi N., Shibano Y.
    Biosci. Biotechnol. Biochem. 60:1388-1389(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Strain: Q268.

Entry informationi

Entry nameiSSIK_STRPT
AccessioniPrimary (citable) accession number: O33702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: October 14, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.