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Protein

Outer membrane protease IcsP

Gene

icsP

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Protease responsible for the cleavage of IcsA between 'Arg-758' and 'Arg-759', removing the entire alpha domain from IscA localized on the bacterial surface. This proteolytic activity contributes to the maintenance of a tight polar cap of IcsA, which is important to Shigella actin-based motility.3 Publications

Catalytic activityi

Preferential cleavage: -Xaa-|-Yaa- in which both Xaa and Yaa are Lys or Arg.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103By similarity1
Active sitei105By similarity1
Active sitei230By similarity1
Active sitei232By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiA26.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protease IcsP (EC:3.4.23.-)
Gene namesi
Name:icsP
Synonyms:sopA
Ordered Locus Names:CP0271
Encoded oniPlasmid pWR1006 Publications
Plasmid pCP3011 Publication
Plasmid pINV_F6_M13821 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002582021 – 315Outer membrane protease IcsPAdd BLAST295

Proteomic databases

PaxDbiO33641.
PRIDEiO33641.

Expressioni

Inductioni

Transcriptionally regulated by VirB (InvE).

Interactioni

Protein-protein interaction databases

STRINGi198214.CP0271.

Structurei

3D structure databases

ProteinModelPortaliO33641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A26 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiCOG4571. LUCA.
HOGENOMiHOG000117799.
KOiK13520.
OMAiHYQTETI.

Family and domain databases

Gene3Di2.40.128.90. 1 hit.
InterProiIPR020080. OM_adhesin/peptidase_omptin.
IPR023619. Peptidase_A26.
IPR020079. Peptidase_A26_CS.
IPR000036. Peptidase_A26_omptin.
[Graphical view]
PfamiPF01278. Omptin. 1 hit.
[Graphical view]
PIRSFiPIRSF001522. Peptidase_A26. 1 hit.
PRINTSiPR00482. OMPTIN.
SUPFAMiSSF69917. SSF69917. 1 hit.
PROSITEiPS00834. OMPTIN_1. 1 hit.
PS00835. OMPTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKFFVLAL CVPAIFTTHA TTNYPLFIPD NISTDISLGS LSGKTKERVY
60 70 80 90 100
HPKEGGRKIS QLDWKYSNAT IVRGGIDWKL IPKVSFGVSG WTTLGNQKAS
110 120 130 140 150
MVDKDWNNSN TPQVWTDQSW HPNTHLRDAN EFELNLKGWL LNNLDYRLGL
160 170 180 190 200
IAGYQESRYS FNAMGGSYIY SENGGSRNKK GAHPSGERTI GYKQLFKIPY
210 220 230 240 250
IGLTANYRHE NFEFGAELKY SGWVLSSDTD KHYQTETIFK DEIKNQNYCS
260 270 280 290 300
VAANIGYYVT PSAKFYIEGS RNYISNKKGD TSLYEQSTNI SGTIKNSASI
310
EYIGFLTSAG IKYIF
Length:315
Mass (Da):35,597
Last modified:February 15, 2005 - v2
Checksum:i26E4C4B091A644E8
GO

Sequence cautioni

The sequence AAB61737 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK18603 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti6F → L in plasmid pINV_F6_M1382. 1
Natural varianti176S → N in plasmid pINV_F6_M1382. 1
Natural varianti182A → T in plasmid pINV_F6_M1382. 1
Natural varianti225L → R in plasmid pINV_F6_M1382. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73461 Genomic DNA. Translation: AAC45084.1.
AF001633 Genomic DNA. Translation: AAB61737.1. Different initiation.
AL391753 Genomic DNA. Translation: CAC05769.2.
AF348706 Genomic DNA. Translation: AAK18603.1. Different initiation.
AY206427 Genomic DNA. Translation: AAP78966.1.
AF386526 Genomic DNA. Translation: AAL72459.2.
RefSeqiNP_858404.2. NC_004851.1.
WP_005061047.1. NZ_LVJC01000149.1.
YP_009062451.1. NC_024996.1.

Genome annotation databases

EnsemblBacteriaiAAL72459; AAL72459; SF_p0271.
GeneIDi1238162.
KEGGisfl:CP0271.
PATRICi18722882. VBIShiFle86970_5532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73461 Genomic DNA. Translation: AAC45084.1.
AF001633 Genomic DNA. Translation: AAB61737.1. Different initiation.
AL391753 Genomic DNA. Translation: CAC05769.2.
AF348706 Genomic DNA. Translation: AAK18603.1. Different initiation.
AY206427 Genomic DNA. Translation: AAP78966.1.
AF386526 Genomic DNA. Translation: AAL72459.2.
RefSeqiNP_858404.2. NC_004851.1.
WP_005061047.1. NZ_LVJC01000149.1.
YP_009062451.1. NC_024996.1.

3D structure databases

ProteinModelPortaliO33641.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.CP0271.

Protein family/group databases

MEROPSiA26.005.

Proteomic databases

PaxDbiO33641.
PRIDEiO33641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL72459; AAL72459; SF_p0271.
GeneIDi1238162.
KEGGisfl:CP0271.
PATRICi18722882. VBIShiFle86970_5532.

Phylogenomic databases

eggNOGiCOG4571. LUCA.
HOGENOMiHOG000117799.
KOiK13520.
OMAiHYQTETI.

Family and domain databases

Gene3Di2.40.128.90. 1 hit.
InterProiIPR020080. OM_adhesin/peptidase_omptin.
IPR023619. Peptidase_A26.
IPR020079. Peptidase_A26_CS.
IPR000036. Peptidase_A26_omptin.
[Graphical view]
PfamiPF01278. Omptin. 1 hit.
[Graphical view]
PIRSFiPIRSF001522. Peptidase_A26. 1 hit.
PRINTSiPR00482. OMPTIN.
SUPFAMiSSF69917. SSF69917. 1 hit.
PROSITEiPS00834. OMPTIN_1. 1 hit.
PS00835. OMPTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICSP_SHIFL
AccessioniPrimary (citable) accession number: O33641
Secondary accession number(s): P95750, Q6XW13, Q7BEI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.