ID   CYSH_RHITR              Reviewed;         180 AA.
AC   O33579;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Phosphoadenosine phosphosulfate reductase;
DE            EC=1.8.4.8;
DE   AltName: Full=PAPS reductase, thioredoxin dependent;
DE   AltName: Full=PAdoPS reductase;
DE   AltName: Full=3'-phosphoadenylylsulfate reductase;
DE   AltName: Full=PAPS sulfotransferase;
DE   Flags: Fragment;
GN   Name=cysH;
OS   Rhizobium tropici.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=398;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CFN 299;
RX   MEDLINE=98446455; PubMed=9773278;
RA   Laeremans T., Martinez-Romero E., Vanderleyden J.;
RT   "Isolation and sequencing of a second Rhizobium tropici CFN299 genetic
RT   locus that contains genes homologous to amino acid sulphate activation
RT   genes.";
RL   DNA Seq. 9:65-70(1998).
CC   -!- FUNCTION: Reduction of activated sulfate into sulfite.
CC   -!- CATALYTIC ACTIVITY: Adenosine 3',5'-bisphosphate + sulfite +
CC       thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
CC   -!- PATHWAY: Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite
CC       from sulfate: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the PAPS reductase family. CysH subfamily.
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DR   EMBL; AJ001223; CAA04617.1; -; Genomic_DNA.
DR   HSSP; P17854; 1SUR.
DR   BRENDA; 1.8.4.8; 258207.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004604; F:phosphoadenylyl-sulfate reductase (thioredo...; IEA:HAMAP.
DR   GO; GO:0016740; F:transferase activity; IEA:InterPro.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0019379; P:sulfate assimilation, phosphoadenylyl sulfa...; IEA:HAMAP.
DR   HAMAP; MF_00063; -; 1.
DR   InterPro; IPR011798; APS_reductase.
DR   InterPro; IPR002500; PAPS_reduct.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF01507; PAPS_reduct; 1.
DR   TIGRFAMs; TIGR02055; APS_reductase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Oxidoreductase.
FT   CHAIN        <1    180       Phosphoadenosine phosphosulfate
FT                                reductase.
FT                                /FTId=PRO_0000100641.
FT   NON_TER       1      1
SQ   SEQUENCE   180 AA;  20395 MW;  F948C70505E461F4 CRC64;
     LQTGRLFPET LKLIDETENQ YDIRISRYEP EQADIDAYAE KYGLNGFYES VEARHACCGV
     RKLKPLARAL SGATIWVTGL RRGQSANRAD TPFAEYDPER NLIKVNPLAD WDIDVIRAYV
     ADNGVPVNPL HQRGYPSIGC EPCTRAIKPG EPERAGRWWW ENDEKRECGL HVHEEAAAAQ
//