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O33525

- MDH_RHIL3

UniProt

O33525 - MDH_RHIL3

Protein

Malate dehydrogenase

Gene

mdh

Organism
Rhizobium leguminosarum bv. viciae (strain 3841)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADBy similarity
    Binding sitei83 – 831SubstrateBy similarity
    Binding sitei89 – 891SubstrateBy similarity
    Binding sitei96 – 961NADBy similarity
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Active sitei176 – 1761Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156NADBy similarity
    Nucleotide bindingi119 – 1213NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciRLEG216596:GKE5-4515-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:RL4439
    OrganismiRhizobium leguminosarum bv. viciae (strain 3841)
    Taxonomic identifieri216596 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
    ProteomesiUP000006575: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320Malate dehydrogenasePRO_0000113462Add
    BLAST

    Proteomic databases

    ProMEXiO33525.

    Interactioni

    Protein-protein interaction databases

    STRINGi216596.RL4439.

    Structurei

    3D structure databases

    ProteinModelPortaliO33525.
    SMRiO33525. Positions 1-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213794.
    KOiK00024.
    OMAiYAPSAFV.
    OrthoDBiEOG6091FG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O33525-1 [UniParc]FASTAAdd to Basket

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    MARNKIALIG SGMIGGTLAH LAGLKELGDI VLFDIADGIP QGKGLDISQS    50
    SPVEGFDVNL TGASDYSAIE GADVCIVTAG VARKPGMSRD DLLGINLKVM 100
    EQVGAGIKKY APNAFVICIT NPLDAMVWAL QKFSGLPANK VVGMAGVLDS 150
    SRFRLFLAKE FNVSVQDVTA FVLGGHGDTM VPLARYSTVG GIPLTDLVTM 200
    GWVTKERLEE IIQRTRDGGA EIVGLLKTGS AYYAPAASAI EMAESYLKDK 250
    KRVLPCAAHL SGQYGVKDMY VGVPTVIGAG GVERIIEIDL NKTEKEAFDK 300
    SVGAVAGLCE ACINIAPALK 320
    Length:320
    Mass (Da):33,590
    Last modified:January 1, 1998 - v1
    Checksum:i2094407A42C2B1C8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ002750 Genomic DNA. Translation: CAA05717.1.
    AM236080 Genomic DNA. Translation: CAK09925.1.
    RefSeqiWP_003543522.1. NC_008380.1.
    YP_770009.1. NC_008380.1.

    Genome annotation databases

    EnsemblBacteriaiCAK09925; CAK09925; RL4439.
    GeneIDi4402981.
    KEGGirle:RL4439.
    PATRICi23145749. VBIRhiLeg32091_5777.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ002750 Genomic DNA. Translation: CAA05717.1 .
    AM236080 Genomic DNA. Translation: CAK09925.1 .
    RefSeqi WP_003543522.1. NC_008380.1.
    YP_770009.1. NC_008380.1.

    3D structure databases

    ProteinModelPortali O33525.
    SMRi O33525. Positions 1-311.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216596.RL4439.

    Proteomic databases

    ProMEXi O33525.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAK09925 ; CAK09925 ; RL4439 .
    GeneIDi 4402981.
    KEGGi rle:RL4439.
    PATRICi 23145749. VBIRhiLeg32091_5777.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213794.
    KOi K00024.
    OMAi YAPSAFV.
    OrthoDBi EOG6091FG.

    Enzyme and pathway databases

    BioCyci RLEG216596:GKE5-4515-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Poole P.S., Allaway D., Smith M.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 3841.

    Entry informationi

    Entry nameiMDH_RHIL3
    AccessioniPrimary (citable) accession number: O33525
    Secondary accession number(s): Q1MAW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3