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Protein

Malate dehydrogenase

Gene

mdh

Organism
Rhizobium leguminosarum bv. viciae (strain 3841)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei83 – 831SubstrateBy similarity
Binding sitei89 – 891SubstrateBy similarity
Binding sitei96 – 961NADBy similarity
Binding sitei121 – 1211SubstrateBy similarity
Binding sitei152 – 1521SubstrateBy similarity
Active sitei176 – 1761Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADBy similarity
Nucleotide bindingi119 – 1213NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciRLEG216596:GKE5-4515-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:RL4439
OrganismiRhizobium leguminosarum bv. viciae (strain 3841)
Taxonomic identifieri216596 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000006575 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Malate dehydrogenasePRO_0000113462Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216596.RL4439.

Structurei

3D structure databases

ProteinModelPortaliO33525.
SMRiO33525. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

O33525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARNKIALIG SGMIGGTLAH LAGLKELGDI VLFDIADGIP QGKGLDISQS
60 70 80 90 100
SPVEGFDVNL TGASDYSAIE GADVCIVTAG VARKPGMSRD DLLGINLKVM
110 120 130 140 150
EQVGAGIKKY APNAFVICIT NPLDAMVWAL QKFSGLPANK VVGMAGVLDS
160 170 180 190 200
SRFRLFLAKE FNVSVQDVTA FVLGGHGDTM VPLARYSTVG GIPLTDLVTM
210 220 230 240 250
GWVTKERLEE IIQRTRDGGA EIVGLLKTGS AYYAPAASAI EMAESYLKDK
260 270 280 290 300
KRVLPCAAHL SGQYGVKDMY VGVPTVIGAG GVERIIEIDL NKTEKEAFDK
310 320
SVGAVAGLCE ACINIAPALK
Length:320
Mass (Da):33,590
Last modified:January 1, 1998 - v1
Checksum:i2094407A42C2B1C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002750 Genomic DNA. Translation: CAA05717.1.
AM236080 Genomic DNA. Translation: CAK09925.1.
RefSeqiWP_003543522.1. NC_008380.1.
YP_770009.1. NC_008380.1.

Genome annotation databases

EnsemblBacteriaiCAK09925; CAK09925; RL4439.
GeneIDi23438169.
KEGGirle:RL4439.
PATRICi23145749. VBIRhiLeg32091_5777.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002750 Genomic DNA. Translation: CAA05717.1.
AM236080 Genomic DNA. Translation: CAK09925.1.
RefSeqiWP_003543522.1. NC_008380.1.
YP_770009.1. NC_008380.1.

3D structure databases

ProteinModelPortaliO33525.
SMRiO33525. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216596.RL4439.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK09925; CAK09925; RL4439.
GeneIDi23438169.
KEGGirle:RL4439.
PATRICi23145749. VBIRhiLeg32091_5777.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciRLEG216596:GKE5-4515-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Poole P.S., Allaway D., Smith M.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 3841.

Entry informationi

Entry nameiMDH_RHIL3
AccessioniPrimary (citable) accession number: O33525
Secondary accession number(s): Q1MAW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.