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Protein

Modification methylase PspPI

Gene

pspPIM

Organism
Psychrobacter sp. (strain TA137)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PspPI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3564 M.PspPI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase PspPI (EC:2.1.1.37)
Short name:
M.PspPI
Alternative name(s):
Cytosine-specific methyltransferase PspPI
Gene namesi
Name:pspPIM
OrganismiPsychrobacter sp. (strain TA137)
Taxonomic identifieri203703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879041 – 416Modification methylase PspPIAdd BLAST416

Structurei

3D structure databases

ProteinModelPortaliO33481
SMRiO33481
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 410SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST334

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

O33481-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFPDNHFSA AIVADTLSVS KRNVETWTQN GKLVPALDPN IHEKPYTKDQ
60 70 80 90 100
LETFEQFSAM FNSSWCEEMA VEPSRSYSLV ELFAGAGGLA LGLEQAGFKS
110 120 130 140 150
VLLNEKDKYA CATLRANRPN WNVIEDDIEN VDFTHLNGKV DLLTGGFPCQ
160 170 180 190 200
PFSYAGKQLG FEDLRGTLVF EMARAIKEIK PKVFLAENVK GLAENDGGRT
210 220 230 240 250
LSIIIKVLED LGYKILEKEV YKAIFYKVPQ KRERLIIIGV RTDLYDKLAY
260 270 280 290 300
EKPSPYYKVL TVADALKAGE LYDVDVPEST GQLYPERKAE IMSYVPEGGY
310 320 330 340 350
WRDLPIRIAK EYMMKSFYLG GGKTGMARRL SWDEPSLTLV CTPAQKQTER
360 370 380 390 400
CHPSESRPLT TREYARIQTF PDDWEFKGSV GQIYKQIGNA VPVNLALAIG
410
KAIIRMLNAA PKDVFE
Length:416
Mass (Da):46,858
Last modified:January 1, 1998 - v1
Checksum:iB4CFD816AD147A9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07554 Genomic DNA Translation: CAA68841.1

Similar proteinsi

Entry informationi

Entry nameiMTP1_PSYTA
AccessioniPrimary (citable) accession number: O33481
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health