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O33481

- MTP1_PSYTA

UniProt

O33481 - MTP1_PSYTA

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Protein

Modification methylase PspPI

Gene

pspPIM

Organism
Psychrobacter sp. (strain TA137)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PspPI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3564. M.PspPI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase PspPI (EC:2.1.1.37)
Short name:
M.PspPI
Alternative name(s):
Cytosine-specific methyltransferase PspPI
Gene namesi
Name:pspPIM
OrganismiPsychrobacter sp. (strain TA137)
Taxonomic identifieri203703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Modification methylase PspPIPRO_0000087904Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO33481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 410334SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33481 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFPDNHFSA AIVADTLSVS KRNVETWTQN GKLVPALDPN IHEKPYTKDQ
60 70 80 90 100
LETFEQFSAM FNSSWCEEMA VEPSRSYSLV ELFAGAGGLA LGLEQAGFKS
110 120 130 140 150
VLLNEKDKYA CATLRANRPN WNVIEDDIEN VDFTHLNGKV DLLTGGFPCQ
160 170 180 190 200
PFSYAGKQLG FEDLRGTLVF EMARAIKEIK PKVFLAENVK GLAENDGGRT
210 220 230 240 250
LSIIIKVLED LGYKILEKEV YKAIFYKVPQ KRERLIIIGV RTDLYDKLAY
260 270 280 290 300
EKPSPYYKVL TVADALKAGE LYDVDVPEST GQLYPERKAE IMSYVPEGGY
310 320 330 340 350
WRDLPIRIAK EYMMKSFYLG GGKTGMARRL SWDEPSLTLV CTPAQKQTER
360 370 380 390 400
CHPSESRPLT TREYARIQTF PDDWEFKGSV GQIYKQIGNA VPVNLALAIG
410
KAIIRMLNAA PKDVFE
Length:416
Mass (Da):46,858
Last modified:January 1, 1998 - v1
Checksum:iB4CFD816AD147A9A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y07554 Genomic DNA. Translation: CAA68841.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y07554 Genomic DNA. Translation: CAA68841.1 .

3D structure databases

ProteinModelPortali O33481.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3564. M.PspPI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the gene encoding PspPI methyltransferase from the Antarctic psychrotroph Psychrobacter sp. strain TA137. Predicted interactions with DNA and organization of the variable region."
    Rina M., Caufrier F., Markaki M., Mavromatis K., Kokkinidis M., Bouriotis V.
    Gene 197:353-360(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTP1_PSYTA
AccessioniPrimary (citable) accession number: O33481
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3