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O33481 (MTP1_PSYTA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase PspPI

Short name=M.PspPI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase PspPI
Gene names
Name:pspPIM
OrganismPsychrobacter sp. (strain TA137)
Taxonomic identifier203703 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PspPI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Modification methylase PspPI
PRO_0000087904

Regions

Domain77 – 410334SAM-dependent MTase C5-type

Sites

Active site1491 By similarity

Sequences

Sequence LengthMass (Da)Tools
O33481 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: B4CFD816AD147A9A

FASTA41646,858
        10         20         30         40         50         60 
MKFPDNHFSA AIVADTLSVS KRNVETWTQN GKLVPALDPN IHEKPYTKDQ LETFEQFSAM 

        70         80         90        100        110        120 
FNSSWCEEMA VEPSRSYSLV ELFAGAGGLA LGLEQAGFKS VLLNEKDKYA CATLRANRPN 

       130        140        150        160        170        180 
WNVIEDDIEN VDFTHLNGKV DLLTGGFPCQ PFSYAGKQLG FEDLRGTLVF EMARAIKEIK 

       190        200        210        220        230        240 
PKVFLAENVK GLAENDGGRT LSIIIKVLED LGYKILEKEV YKAIFYKVPQ KRERLIIIGV 

       250        260        270        280        290        300 
RTDLYDKLAY EKPSPYYKVL TVADALKAGE LYDVDVPEST GQLYPERKAE IMSYVPEGGY 

       310        320        330        340        350        360 
WRDLPIRIAK EYMMKSFYLG GGKTGMARRL SWDEPSLTLV CTPAQKQTER CHPSESRPLT 

       370        380        390        400        410 
TREYARIQTF PDDWEFKGSV GQIYKQIGNA VPVNLALAIG KAIIRMLNAA PKDVFE 

« Hide

References

[1]"Cloning and characterization of the gene encoding PspPI methyltransferase from the Antarctic psychrotroph Psychrobacter sp. strain TA137. Predicted interactions with DNA and organization of the variable region."
Rina M., Caufrier F., Markaki M., Mavromatis K., Kokkinidis M., Bouriotis V.
Gene 197:353-360(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y07554 Genomic DNA. Translation: CAA68841.1.

3D structure databases

ProteinModelPortalO33481.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3564. M.PspPI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTP1_PSYTA
AccessionPrimary (citable) accession number: O33481
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries