O33481 (MTP1_PSYTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase PspPI
Short name=M.PspPI
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase PspPI

Gene names

Name:

pspPIM

Organism

Psychrobacter sp. (strain TA137)

Taxonomic identifier

203703 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PspPI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Modification methylase PspPI		PRO_0000087904
Sites
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33481 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B4CFD816AD147A9A	FASTA	416	46,858
	10 20 30 40 50 60 MKFPDNHFSA AIVADTLSVS KRNVETWTQN GKLVPALDPN IHEKPYTKDQ LETFEQFSAM 70 80 90 100 110 120 FNSSWCEEMA VEPSRSYSLV ELFAGAGGLA LGLEQAGFKS VLLNEKDKYA CATLRANRPN 130 140 150 160 170 180 WNVIEDDIEN VDFTHLNGKV DLLTGGFPCQ PFSYAGKQLG FEDLRGTLVF EMARAIKEIK 190 200 210 220 230 240 PKVFLAENVK GLAENDGGRT LSIIIKVLED LGYKILEKEV YKAIFYKVPQ KRERLIIIGV 250 260 270 280 290 300 RTDLYDKLAY EKPSPYYKVL TVADALKAGE LYDVDVPEST GQLYPERKAE IMSYVPEGGY 310 320 330 340 350 360 WRDLPIRIAK EYMMKSFYLG GGKTGMARRL SWDEPSLTLV CTPAQKQTER CHPSESRPLT 370 380 390 400 410 TREYARIQTF PDDWEFKGSV GQIYKQIGNA VPVNLALAIG KAIIRMLNAA PKDVFE

References

[1]

"Cloning and characterization of the gene encoding PspPI methyltransferase from the Antarctic psychrotroph Psychrobacter sp. strain TA137. Predicted interactions with DNA and organization of the variable region." Rina M., Caufrier F., Markaki M., Mavromatis K., Kokkinidis M., Bouriotis V. Gene 197:353-360(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07554 Genomic DNA. Translation: CAA68841.1.
3D structure databases
ProteinModelPortal	O33481.
ModBase	Search...
Protein family/group databases
REBASE	3564. M.PspPI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTP1_PSYTA

Accession

Primary (citable) accession number: O33481

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program