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Protein

Esterase EstA

Gene

estA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Esterase whose enzymatic activity is required for rhamnolipid production, all kinds of cell motility (swimming, swarming, and twitching), and biofilm formation; the exact role of EstA in these processes is unclear. In vitro, has pronounced esterase activities towards p-nitrophenyl esters of short acyl chain length (C4-C6) and Tween detergents. Also shows relatively high activity towards beta-naphthyl butyrate, whereas its activities towards triacylglycerols and acyls-CoA are negligible.2 Publications

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.2 Publications

Kineticsi

  1. KM=0.7 mM for p-nitrophenyl butyrate1 Publication
  1. Vmax=220 µmol/min/mg enzyme with p-nitrophenyl butyrate as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei38 – 381NucleophileCurated
Active sitei310 – 3101Curated
Active sitei313 – 3131Curated

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB
  • lipase activity Source: InterPro

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: PseudoCAP
  • bacterial-type flagellum-dependent swarming motility Source: PseudoCAP
  • biofilm formation Source: UniProtKB
  • cell motility Source: UniProtKB
  • glycolipid biosynthetic process Source: PseudoCAP
  • lipid biosynthetic process Source: UniProtKB
  • single-species biofilm formation Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BRENDAi3.1.1.1. 5087.

Protein family/group databases

TCDBi1.B.12.5.9. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Esterase EstA (EC:3.1.1.1)
Alternative name(s):
Autotransporter esterase EstA
Gene namesi
Name:estA
Synonyms:papA
Ordered Locus Names:PA5112
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5112.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 397373ExtracellularSequence analysisAdd
BLAST
Transmembranei398 – 40811Beta strandedSequence analysisAdd
BLAST
Topological domaini409 – 4102PeriplasmicSequence analysis
Transmembranei411 – 42111Beta strandedSequence analysisAdd
BLAST
Topological domaini422 – 43716ExtracellularSequence analysisAdd
BLAST
Transmembranei438 – 44710Beta strandedSequence analysis
Topological domaini448 – 4514PeriplasmicSequence analysis
Transmembranei452 – 46110Beta strandedSequence analysis
Topological domaini462 – 48827ExtracellularSequence analysisAdd
BLAST
Transmembranei489 – 50012Beta strandedSequence analysisAdd
BLAST
Topological domaini501 – 5077PeriplasmicSequence analysis
Transmembranei508 – 51811Beta strandedSequence analysisAdd
BLAST
Topological domaini519 – 54729ExtracellularSequence analysisAdd
BLAST
Transmembranei548 – 55811Beta strandedSequence analysisAdd
BLAST
Topological domaini559 – 5613PeriplasmicSequence analysis
Transmembranei562 – 57110Beta strandedSequence analysis
Topological domaini572 – 60534ExtracellularSequence analysisAdd
BLAST
Transmembranei606 – 61510Beta strandedSequence analysis
Topological domaini616 – 6183PeriplasmicSequence analysis
Transmembranei619 – 62810Beta strandedSequence analysis
Topological domaini629 – 6368ExtracellularSequence analysis
Transmembranei637 – 64610Beta strandedSequence analysis

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Strains lacking this gene produce only marginal amounts of extracellular rhamnolipids. They also show no swarming motility, and both other forms of surface motility, swimming and twitching, are completely absent in these mutant cells. Biofilm formation is also affected.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381S → A: Loss of catalytic activity. Fails to complement the estA mutant phenotypes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 646622Esterase EstAPRO_0000017845Add
BLAST

Proteomic databases

PaxDbiO33407.
PRIDEiO33407.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5112.

Structurei

Secondary structure

1
646
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 354Combined sources
Turni38 – 403Combined sources
Beta strandi63 – 653Combined sources
Helixi78 – 858Combined sources
Helixi90 – 934Combined sources
Beta strandi94 – 974Combined sources
Helixi99 – 1046Combined sources
Helixi119 – 1279Combined sources
Beta strandi132 – 1365Combined sources
Beta strandi139 – 1446Combined sources
Helixi147 – 1526Combined sources
Turni153 – 1553Combined sources
Beta strandi162 – 1665Combined sources
Helixi170 – 1745Combined sources
Helixi181 – 20020Combined sources
Beta strandi206 – 2094Combined sources
Helixi214 – 2163Combined sources
Turni218 – 2225Combined sources
Helixi226 – 24722Combined sources
Beta strandi251 – 2544Combined sources
Helixi256 – 26510Combined sources
Helixi267 – 2704Combined sources
Turni278 – 2803Combined sources
Beta strandi282 – 2843Combined sources
Turni292 – 2943Combined sources
Beta strandi298 – 3003Combined sources
Helixi303 – 3053Combined sources
Beta strandi307 – 3126Combined sources
Helixi316 – 33116Combined sources
Helixi333 – 3364Combined sources
Helixi339 – 36123Combined sources
Beta strandi372 – 38413Combined sources
Beta strandi393 – 40715Combined sources
Beta strandi409 – 42719Combined sources
Turni428 – 4314Combined sources
Beta strandi432 – 44918Combined sources
Beta strandi452 – 47423Combined sources
Beta strandi477 – 49822Combined sources
Beta strandi507 – 52317Combined sources
Beta strandi536 – 5383Combined sources
Beta strandi541 – 57434Combined sources
Beta strandi580 – 5856Combined sources
Beta strandi593 – 5964Combined sources
Beta strandi602 – 61615Combined sources
Beta strandi619 – 63012Combined sources
Beta strandi633 – 64513Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KVNX-ray2.50A/X25-646[»]
ProteinModelPortaliO33407.
SMRiO33407. Positions 25-646.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33407.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini366 – 646281AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

Contains a C-terminal autotransporter domain that integrates into the outer membrane and enables the translocation of the catalytic N-terminal domain to the bacterial cell surface.1 Publication

Sequence similaritiesi

Belongs to the 'GDSL' lipolytic enzyme family.Curated
Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108FHP. Bacteria.
COG3240. LUCA.
HOGENOMiHOG000267346.
InParanoidiO33407.
KOiK12686.
OMAiKRTFALG.
OrthoDBiEOG6R2GV6.
PhylomeDBiO33407.

Family and domain databases

Gene3Di2.40.128.130. 1 hit.
3.40.50.1110. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR001087. GDSL.
IPR017186. Lipase_autotranspt_EstA.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF00657. Lipase_GDSL. 1 hit.
[Graphical view]
PIRSFiPIRSF037375. Autotrns_EstA. 1 hit.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF52266. SSF52266. 4 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS01098. LIPASE_GDSL_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRMALKPLV AACLLASLST APQAAPSPYS TLVVFGDSLS DAGQFPDPAG
60 70 80 90 100
PAGSTSRFTN RVGPTYQNGS GEIFGPTAPM LLGNQLGIAP GDLAASTSPV
110 120 130 140 150
NAQQGIADGN NWAVGGYRTD QIYDSITAAN GSLIERDNTL LRSRDGYLVD
160 170 180 190 200
RARQGLGADP NALYYITGGG NDFLQGRILN DVQAQQAAGR LVDSVQALQQ
210 220 230 240 250
AGARYIVVWL LPDLGLTPAT FGGPLQPFAS QLSGTFNAEL TAQLSQAGAN
260 270 280 290 300
VIPLNIPLLL KEGMANPASF GLAADQNLIG TCFSGNGCTM NPTYGINGST
310 320 330 340 350
PDPSKLLFND SVHPTITGQR LIADYTYSLL SAPWELTLLP EMAHGTLRAY
360 370 380 390 400
QDELRSQWQA DWENWQNVGQ WRGFVGGGGQ RLDFDSQDSA ASGDGNGYNL
410 420 430 440 450
TLGGSYRIDE AWRAGVAAGF YRQKLEAGAK DSDYRMNSYM ASAFVQYQEN
460 470 480 490 500
RWWADAALTG GYLDYDDLKR KFALGGGERS EKGDTNGHLW AFSARLGYDI
510 520 530 540 550
AQQADSPWHL SPFVSADYAR VEVDGYSEKG ASATALDYDD QKRSSKRLGA
560 570 580 590 600
GLQGKYAFGS DTQLFAEYAH EREYEDDTQD LTMSLNSLPG NRFTLEGYTP
610 620 630 640
QDHLNRVSLG FSQKLAPELS LRGGYNWRKG EDDTQQSVSL ALSLDF
Length:646
Mass (Da):69,609
Last modified:January 1, 1998 - v1
Checksum:iAFF3CC4FFE25A191
GO

Sequence cautioni

The sequence CAC14200.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005091 Genomic DNA. Translation: AAB61674.1.
AJ277638 Genomic DNA. Translation: CAC14200.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG08497.1.
PIRiG83006.
RefSeqiNP_253799.1. NC_002516.2.
WP_003115524.1. NZ_ASJY01000805.1.

Genome annotation databases

EnsemblBacteriaiAAG08497; AAG08497; PA5112.
GeneIDi878826.
KEGGipae:PA5112.
PATRICi19845057. VBIPseAer58763_5357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005091 Genomic DNA. Translation: AAB61674.1.
AJ277638 Genomic DNA. Translation: CAC14200.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG08497.1.
PIRiG83006.
RefSeqiNP_253799.1. NC_002516.2.
WP_003115524.1. NZ_ASJY01000805.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KVNX-ray2.50A/X25-646[»]
ProteinModelPortaliO33407.
SMRiO33407. Positions 25-646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5112.

Protein family/group databases

TCDBi1.B.12.5.9. the autotransporter-1 (at-1) family.

Proteomic databases

PaxDbiO33407.
PRIDEiO33407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08497; AAG08497; PA5112.
GeneIDi878826.
KEGGipae:PA5112.
PATRICi19845057. VBIPseAer58763_5357.

Organism-specific databases

PseudoCAPiPA5112.

Phylogenomic databases

eggNOGiENOG4108FHP. Bacteria.
COG3240. LUCA.
HOGENOMiHOG000267346.
InParanoidiO33407.
KOiK12686.
OMAiKRTFALG.
OrthoDBiEOG6R2GV6.
PhylomeDBiO33407.

Enzyme and pathway databases

BRENDAi3.1.1.1. 5087.

Miscellaneous databases

EvolutionaryTraceiO33407.

Family and domain databases

Gene3Di2.40.128.130. 1 hit.
3.40.50.1110. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR001087. GDSL.
IPR017186. Lipase_autotranspt_EstA.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF00657. Lipase_GDSL. 1 hit.
[Graphical view]
PIRSFiPIRSF037375. Autotrns_EstA. 1 hit.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF52266. SSF52266. 4 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS01098. LIPASE_GDSL_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa."
    Wilhelm S., Tommassen J., Jaeger K.-E.
    J. Bacteriol. 181:6977-6986(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Evolutionary origins of the autotransporter proteins."
    Henderson I.R., Nataro J.P., Cappello R., Stein C.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  4. "The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation."
    Wilhelm S., Gdynia A., Tielen P., Rosenau F., Jaeger K.E.
    J. Bacteriol. 189:6695-6703(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF SER-38.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  5. Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS.
  6. "Crystal structure of a full-length autotransporter."
    van den Berg B.
    J. Mol. Biol. 396:627-633(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 25-646, DOMAIN.

Entry informationi

Entry nameiESTA_PSEAE
AccessioniPrimary (citable) accession number: O33407
Secondary accession number(s): Q7DC45, Q9F3Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 1, 1998
Last modified: January 20, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression of EstA results in an increased production of rhamnolipids.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.