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Protein

Esterase EstA

Gene

estA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Esterase whose enzymatic activity is required for rhamnolipid production, all kinds of cell motility (swimming, swarming, and twitching), and biofilm formation; the exact role of EstA in these processes is unclear. In vitro, has pronounced esterase activities towards p-nitrophenyl esters of short acyl chain length (C4-C6) and Tween detergents. Also shows relatively high activity towards beta-naphthyl butyrate, whereas its activities towards triacylglycerols and acyls-CoA are negligible.2 Publications

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.2 Publications

Kineticsi

  1. KM=0.7 mM for p-nitrophenyl butyrate1 Publication
  1. Vmax=220 µmol/min/mg enzyme with p-nitrophenyl butyrate as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38NucleophileCurated1
Active sitei310Curated1
Active sitei313Curated1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB
  • lipase activity Source: InterPro

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: PseudoCAP
  • bacterial-type flagellum-dependent swarming motility Source: PseudoCAP
  • biofilm formation Source: UniProtKB
  • cell motility Source: UniProtKB
  • glycolipid biosynthetic process Source: PseudoCAP
  • lipid biosynthetic process Source: UniProtKB
  • single-species biofilm formation Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BRENDAi3.1.1.1. 5087.

Protein family/group databases

TCDBi1.B.12.5.9. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Esterase EstA (EC:3.1.1.1)
Alternative name(s):
Autotransporter esterase EstA
Gene namesi
Name:estA
Synonyms:papA
Ordered Locus Names:PA5112
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5112.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 397ExtracellularSequence analysisAdd BLAST373
Transmembranei398 – 408Beta strandedSequence analysisAdd BLAST11
Topological domaini409 – 410PeriplasmicSequence analysis2
Transmembranei411 – 421Beta strandedSequence analysisAdd BLAST11
Topological domaini422 – 437ExtracellularSequence analysisAdd BLAST16
Transmembranei438 – 447Beta strandedSequence analysis10
Topological domaini448 – 451PeriplasmicSequence analysis4
Transmembranei452 – 461Beta strandedSequence analysis10
Topological domaini462 – 488ExtracellularSequence analysisAdd BLAST27
Transmembranei489 – 500Beta strandedSequence analysisAdd BLAST12
Topological domaini501 – 507PeriplasmicSequence analysis7
Transmembranei508 – 518Beta strandedSequence analysisAdd BLAST11
Topological domaini519 – 547ExtracellularSequence analysisAdd BLAST29
Transmembranei548 – 558Beta strandedSequence analysisAdd BLAST11
Topological domaini559 – 561PeriplasmicSequence analysis3
Transmembranei562 – 571Beta strandedSequence analysis10
Topological domaini572 – 605ExtracellularSequence analysisAdd BLAST34
Transmembranei606 – 615Beta strandedSequence analysis10
Topological domaini616 – 618PeriplasmicSequence analysis3
Transmembranei619 – 628Beta strandedSequence analysis10
Topological domaini629 – 636ExtracellularSequence analysis8
Transmembranei637 – 646Beta strandedSequence analysis10

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Strains lacking this gene produce only marginal amounts of extracellular rhamnolipids. They also show no swarming motility, and both other forms of surface motility, swimming and twitching, are completely absent in these mutant cells. Biofilm formation is also affected.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38S → A: Loss of catalytic activity. Fails to complement the estA mutant phenotypes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001784525 – 646Esterase EstAAdd BLAST622

Proteomic databases

PaxDbiO33407.
PRIDEiO33407.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5112.

Structurei

Secondary structure

1646
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 35Combined sources4
Turni38 – 40Combined sources3
Beta strandi63 – 65Combined sources3
Helixi78 – 85Combined sources8
Helixi90 – 93Combined sources4
Beta strandi94 – 97Combined sources4
Helixi99 – 104Combined sources6
Helixi119 – 127Combined sources9
Beta strandi132 – 136Combined sources5
Beta strandi139 – 144Combined sources6
Helixi147 – 152Combined sources6
Turni153 – 155Combined sources3
Beta strandi162 – 166Combined sources5
Helixi170 – 174Combined sources5
Helixi181 – 200Combined sources20
Beta strandi206 – 209Combined sources4
Helixi214 – 216Combined sources3
Turni218 – 222Combined sources5
Helixi226 – 247Combined sources22
Beta strandi251 – 254Combined sources4
Helixi256 – 265Combined sources10
Helixi267 – 270Combined sources4
Turni278 – 280Combined sources3
Beta strandi282 – 284Combined sources3
Turni292 – 294Combined sources3
Beta strandi298 – 300Combined sources3
Helixi303 – 305Combined sources3
Beta strandi307 – 312Combined sources6
Helixi316 – 331Combined sources16
Helixi333 – 336Combined sources4
Helixi339 – 361Combined sources23
Beta strandi372 – 384Combined sources13
Beta strandi393 – 407Combined sources15
Beta strandi409 – 427Combined sources19
Turni428 – 431Combined sources4
Beta strandi432 – 449Combined sources18
Beta strandi452 – 474Combined sources23
Beta strandi477 – 498Combined sources22
Beta strandi507 – 523Combined sources17
Beta strandi536 – 538Combined sources3
Beta strandi541 – 574Combined sources34
Beta strandi580 – 585Combined sources6
Beta strandi593 – 596Combined sources4
Beta strandi602 – 616Combined sources15
Beta strandi619 – 630Combined sources12
Beta strandi633 – 645Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KVNX-ray2.50A/X25-646[»]
ProteinModelPortaliO33407.
SMRiO33407.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33407.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini366 – 646AutotransporterPROSITE-ProRule annotationAdd BLAST281

Domaini

Contains a C-terminal autotransporter domain that integrates into the outer membrane and enables the translocation of the catalytic N-terminal domain to the bacterial cell surface.1 Publication

Sequence similaritiesi

Belongs to the 'GDSL' lipolytic enzyme family.Curated
Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108FHP. Bacteria.
COG3240. LUCA.
HOGENOMiHOG000267346.
InParanoidiO33407.
KOiK12686.
OMAiKRTFALG.
PhylomeDBiO33407.

Family and domain databases

Gene3Di2.40.128.130. 1 hit.
3.40.50.1110. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR001087. GDSL.
IPR017186. Lipase_autotranspt_EstA.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF00657. Lipase_GDSL. 1 hit.
[Graphical view]
PIRSFiPIRSF037375. Autotrns_EstA. 1 hit.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF52266. SSF52266. 4 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS01098. LIPASE_GDSL_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRMALKPLV AACLLASLST APQAAPSPYS TLVVFGDSLS DAGQFPDPAG
60 70 80 90 100
PAGSTSRFTN RVGPTYQNGS GEIFGPTAPM LLGNQLGIAP GDLAASTSPV
110 120 130 140 150
NAQQGIADGN NWAVGGYRTD QIYDSITAAN GSLIERDNTL LRSRDGYLVD
160 170 180 190 200
RARQGLGADP NALYYITGGG NDFLQGRILN DVQAQQAAGR LVDSVQALQQ
210 220 230 240 250
AGARYIVVWL LPDLGLTPAT FGGPLQPFAS QLSGTFNAEL TAQLSQAGAN
260 270 280 290 300
VIPLNIPLLL KEGMANPASF GLAADQNLIG TCFSGNGCTM NPTYGINGST
310 320 330 340 350
PDPSKLLFND SVHPTITGQR LIADYTYSLL SAPWELTLLP EMAHGTLRAY
360 370 380 390 400
QDELRSQWQA DWENWQNVGQ WRGFVGGGGQ RLDFDSQDSA ASGDGNGYNL
410 420 430 440 450
TLGGSYRIDE AWRAGVAAGF YRQKLEAGAK DSDYRMNSYM ASAFVQYQEN
460 470 480 490 500
RWWADAALTG GYLDYDDLKR KFALGGGERS EKGDTNGHLW AFSARLGYDI
510 520 530 540 550
AQQADSPWHL SPFVSADYAR VEVDGYSEKG ASATALDYDD QKRSSKRLGA
560 570 580 590 600
GLQGKYAFGS DTQLFAEYAH EREYEDDTQD LTMSLNSLPG NRFTLEGYTP
610 620 630 640
QDHLNRVSLG FSQKLAPELS LRGGYNWRKG EDDTQQSVSL ALSLDF
Length:646
Mass (Da):69,609
Last modified:January 1, 1998 - v1
Checksum:iAFF3CC4FFE25A191
GO

Sequence cautioni

The sequence CAC14200 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005091 Genomic DNA. Translation: AAB61674.1.
AJ277638 Genomic DNA. Translation: CAC14200.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG08497.1.
PIRiG83006.
RefSeqiNP_253799.1. NC_002516.2.
WP_003115524.1. NZ_ASJY01000805.1.

Genome annotation databases

EnsemblBacteriaiAAG08497; AAG08497; PA5112.
GeneIDi878826.
KEGGipae:PA5112.
PATRICi19845057. VBIPseAer58763_5357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005091 Genomic DNA. Translation: AAB61674.1.
AJ277638 Genomic DNA. Translation: CAC14200.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG08497.1.
PIRiG83006.
RefSeqiNP_253799.1. NC_002516.2.
WP_003115524.1. NZ_ASJY01000805.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KVNX-ray2.50A/X25-646[»]
ProteinModelPortaliO33407.
SMRiO33407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5112.

Protein family/group databases

TCDBi1.B.12.5.9. the autotransporter-1 (at-1) family.

Proteomic databases

PaxDbiO33407.
PRIDEiO33407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08497; AAG08497; PA5112.
GeneIDi878826.
KEGGipae:PA5112.
PATRICi19845057. VBIPseAer58763_5357.

Organism-specific databases

PseudoCAPiPA5112.

Phylogenomic databases

eggNOGiENOG4108FHP. Bacteria.
COG3240. LUCA.
HOGENOMiHOG000267346.
InParanoidiO33407.
KOiK12686.
OMAiKRTFALG.
PhylomeDBiO33407.

Enzyme and pathway databases

BRENDAi3.1.1.1. 5087.

Miscellaneous databases

EvolutionaryTraceiO33407.

Family and domain databases

Gene3Di2.40.128.130. 1 hit.
3.40.50.1110. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR001087. GDSL.
IPR017186. Lipase_autotranspt_EstA.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF00657. Lipase_GDSL. 1 hit.
[Graphical view]
PIRSFiPIRSF037375. Autotrns_EstA. 1 hit.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF52266. SSF52266. 4 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS01098. LIPASE_GDSL_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESTA_PSEAE
AccessioniPrimary (citable) accession number: O33407
Secondary accession number(s): Q7DC45, Q9F3Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression of EstA results in an increased production of rhamnolipids.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.