ID   MBHL_OLICO              Reviewed;         604 AA.
AC   O33406; Q6LB92;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Uptake hydrogenase large subunit;
DE            EC=1.12.99.6;
DE   AltName: Full=Hydrogenlyase;
DE   AltName: Full=Membrane-bound hydrogenase large subunit;
GN   Name=hoxL; OrderedLocusNames=OCAR_p_08063;
OS   Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5).
OG   Plasmid megaplasmid pHCG3.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Oligotropha.
OX   NCBI_TaxID=504832;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-28.
RX   MEDLINE=97464431; PubMed=9324252;
RA   Santiago B., Meyer O.;
RT   "Purification and molecular characterization of the H2 uptake
RT   membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium
RT   Oligotropha carboxidovorans.";
RL   J. Bacteriol. 179:6053-6060(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=14644498; DOI=10.1016/j.gene.2003.08.027;
RA   Fuhrmann S., Ferner M., Jeffke T., Henne A., Gottschalk G., Meyer O.;
RT   "Complete nucleotide sequence of the circular megaplasmid pHCG3 of
RT   Oligotropha carboxidovorans: function in the chemolithoautotrophic
RT   utilization of CO, H(2) and CO(2).";
RL   Gene 322:67-75(2003).
CC   -!- FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to
CC       increase the production of ATP and to protect nitrogenase against
CC       inhibition or damage by O(2) under carbon- or phosphate-limited
CC       conditions (By similarity).
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 nickel ion per subunit (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC       subunit family.
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DR   EMBL; X82447; CAG28467.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_015634.1; -.
DR   HSSP; P21852; 1UBL.
DR   GeneID; 2807125; -.
DR   GenomeReviews; X82447_GR; OCAR_p_08063.
DR   BRENDA; 1.12.99.6; 255922.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0016151; F:nickel ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Complete proteome; Direct protein sequencing; Membrane;
KW   Metal-binding; Nickel; Oxidoreductase; Plasmid.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    604       Uptake hydrogenase large subunit.
FT                                /FTId=PRO_0000199714.
FT   METAL        76     76       Nickel (Potential).
FT   METAL        79     79       Nickel (Potential).
FT   METAL       583    583       Nickel (Potential).
FT   METAL       586    586       Nickel (Potential).
SQ   SEQUENCE   604 AA;  67144 MW;  F4C5271D2B241535 CRC64;
     MSVIQTPNGY KLDNSGRRVV VDPVTRIEGH MRCEVNVDSN NVIRNAVSTG TMWRGLEVIL
     KGRDPRDAWA FVERICGVCT GCHALASVRA VENALDIRIP PNAHLIREIM AKVLQWHDHV
     VHFYHLHALD WVNPVNALKA DPKATSELQQ LVGPNHPMSS PGYFRDIQNR LKRFVESGEL
     GIFKNGYWDN PAYKLSPEAD LMATAHYLEA LDIQKEIVKI HTIFGGKNPH PNFMVGGVPC
     AINMDGDLAA GAPLNMERLN FVRARIEEAY EFSKNVYIPD VIAIATFYKG WLYGGGLSAT
     NVMDYGDYAK VNYDKSTDQL KGGAILNGNW NEVFPVDAAD PEQIQEFVAH SWYKYPDEAK
     GLHPWDGVTE HNYALGPNTK GTRTDIKQLD EAAKYSWIKS PRWRGHAVEV GPLSRYILNY
     AQGNQYVIEQ VDSSLAAFNK LAGTNLTPKQ ALPSTIGRTL ARALEAHYCA AMMLDDWKEL
     IGNIKAGDSS TANVEKWDPS TWPKEAKGYG LVAAPRGANG HWIRIKDGKI ANYQCIVPTT
     WNGSPRDPAG NIGAFEASLM NTPMERPEEP VEILRTLHSF DPCLACSTHV MSEDGENLAK
     VTVR
//