Reviewed, UniProtKB/Swiss-Prot O33406 (MBHL_OLICO)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase large subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase large subunit

Gene names

Name:	hoxL
Ordered Locus Names:	OCAR_p_08063

Encoded on

Plasmid megaplasmid pHCG3

Organism

Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]

Taxonomic identifier

504832 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions By similarity.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 604	603	Uptake hydrogenase large subunit		PRO_0000199714
Sites
	Metal binding	76	1	Nickel Potential
	Metal binding	79	1	Nickel Potential
	Metal binding	583	1	Nickel Potential
	Metal binding	586	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	O33406-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: F4C5271D2B241535	FASTA	604	67,144
	10 20 30 40 50 60 MSVIQTPNGY KLDNSGRRVV VDPVTRIEGH MRCEVNVDSN NVIRNAVSTG TMWRGLEVIL 70 80 90 100 110 120 KGRDPRDAWA FVERICGVCT GCHALASVRA VENALDIRIP PNAHLIREIM AKVLQWHDHV 130 140 150 160 170 180 VHFYHLHALD WVNPVNALKA DPKATSELQQ LVGPNHPMSS PGYFRDIQNR LKRFVESGEL 190 200 210 220 230 240 GIFKNGYWDN PAYKLSPEAD LMATAHYLEA LDIQKEIVKI HTIFGGKNPH PNFMVGGVPC 250 260 270 280 290 300 AINMDGDLAA GAPLNMERLN FVRARIEEAY EFSKNVYIPD VIAIATFYKG WLYGGGLSAT 310 320 330 340 350 360 NVMDYGDYAK VNYDKSTDQL KGGAILNGNW NEVFPVDAAD PEQIQEFVAH SWYKYPDEAK 370 380 390 400 410 420 GLHPWDGVTE HNYALGPNTK GTRTDIKQLD EAAKYSWIKS PRWRGHAVEV GPLSRYILNY 430 440 450 460 470 480 AQGNQYVIEQ VDSSLAAFNK LAGTNLTPKQ ALPSTIGRTL ARALEAHYCA AMMLDDWKEL 490 500 510 520 530 540 IGNIKAGDSS TANVEKWDPS TWPKEAKGYG LVAAPRGANG HWIRIKDGKI ANYQCIVPTT 550 560 570 580 590 600 WNGSPRDPAG NIGAFEASLM NTPMERPEEP VEILRTLHSF DPCLACSTHV MSEDGENLAK VTVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and molecular characterization of the H2 uptake membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium Oligotropha carboxidovorans." Santiago B., Meyer O. J. Bacteriol. 179:6053-6060(1997) [PubMed: 9324252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-28.
[2]	"Complete nucleotide sequence of the circular megaplasmid pHCG3 of Oligotropha carboxidovorans: function in the chemolithoautotrophic utilization of CO, H(2) and CO(2)." Fuhrmann S., Ferner M., Jeffke T., Henne A., Gottschalk G., Meyer O. Gene 322:67-75(2003) [PubMed: 14644498] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X82447 Genomic DNA. Translation: CAG28467.1. Different initiation.
RefSeq	YP_015634.1.
3D structure databases
HSSP	HSSP built from PDB template 1UBL based on UniProtKB P21852.
ModBase	Search...
Genome annotation databases
GeneID	2807125.
GenomeReviews	Gene locus OCAR_p_08063 in contig X82447_GR.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 255922.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_OLICO

Accession

Primary (citable) accession number: O33406
Secondary accession number(s): Q6LB92

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 54 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)