Uptake hydrogenase small subunit precursor - Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)

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Reviewed, UniProtKB/Swiss-Prot O33405 (MBHS_OLICO)

Last modified October 13, 2009. Version 68. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit

Gene names

Name:	hoxS
Ordered Locus Names:	OCAR_p_08064

Encoded on

Plasmid megaplasmid pHCG3

Organism

Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]

Taxonomic identifier

504832 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha

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Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This enzyme recycles the H₂ produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O₂ under carbon- or phosphate-limited conditions By similarity.
Catalytic activity	H₂ + A = AH₂.
Cofactor	Binds 2 4Fe-4S clusters. Binds 1 3Fe-4S cluster.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 45

Tat-type signal Ref.1

Chain

46 – 360

315

Uptake hydrogenase small subunit

PRO_0000013433

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

160

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

194

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

232

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

235

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

260

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

266

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

275

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

294

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

297

Iron-sulfur 3 (3Fe-4S) By similarity

Experimental info

Sequence conflict

C → E AA sequence Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

O33405-1 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: F4D5CB57BC1D1DA2
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FASTA

360

39,322

        10         20         30         40         50         60 
MTPTETFYEV MRRQGVTRRS FLKFCSLTAT ALGLGPAYTS EIAHAMETKP RTPVLWLHGL 

        70         80         90        100        110        120 
ECTCCSESFI RSAHPLVKDV VLSMISLDYD DTLMAAAGHQ AEAALADTIE RYKGNYILAV 

       130        140        150        160        170        180 
EGNPPLNEDG MFCIIGGKPF VDQLRYAAKH AKAIISWGSC ASHGCVQAAR PNPTRATPVH 

       190        200        210        220        230        240 
QVITDKPIIK VPGCPPIAEV MTGVITYMLT FGKLPELDRT GRPKMFYSQR IHDKCYRRPH 

       250        260        270        280        290        300 
FDAGQFVESF DDEGARRGYC LYKVGCKGPT TYNACSTIRW NEGTSFPIQA GHGCIGCSEE 

       310        320        330        340        350        360 
GFWDKGSWYA RLQDIHQFGI EANADQIGGT VAVGAAGAVA AHAAVSALKR AQTKRQTTTS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and molecular characterization of the H2 uptake membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium Oligotropha carboxidovorans." Santiago B., Meyer O. J. Bacteriol. 179:6053-6060(1997) [PubMed: 9324252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-63.
[2]	"Complete nucleotide sequence of the circular megaplasmid pHCG3 of Oligotropha carboxidovorans: function in the chemolithoautotrophic utilization of CO, H(2) and CO(2)." Fuhrmann S., Ferner M., Jeffke T., Henne A., Gottschalk G., Meyer O. Gene 322:67-75(2003) [PubMed: 14644498] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	X82447 Genomic DNA. Translation: CAG28468.1.
RefSeq	YP_015635.2.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Genome annotation databases
GeneID	2807127.
GenomeReviews	Gene locus OCAR_p_08064 in contig X82447_GR.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 255922.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MBHS_OLICO

Accession

Primary (citable) accession number: O33405
Secondary accession number(s): Q6LB91

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	July 19, 2004
Last modified:	October 13, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents