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Protein

Uptake hydrogenase small subunit

Gene

hoxS

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.By similarity

Catalytic activityi

H2 + A = AH2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi65Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi160Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi194Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi232Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity1
Metal bindingi235Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi260Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi266Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi275Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi294Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi297Iron-sulfur 3 (3Fe-4S)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Uptake hydrogenase small subunit (EC:1.12.99.6)
Alternative name(s):
Hydrogenlyase
Membrane-bound hydrogenase small subunit
Gene namesi
Name:hoxS
Ordered Locus Names:OCA5_pHCG300640
Encoded oniPlasmid megaplasmid pHCG30 Publication
OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Taxonomic identifieri504832 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
Proteomesi
  • UP000007730 Componenti: Plasmid pHCG3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 45Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST45
ChainiPRO_000001343346 – 367Uptake hydrogenase small subunitAdd BLAST322

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK06282.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPTETFYEV MRRQGVTRRS FLKFCSLTAT ALGLGPAYTS EIAHAMETKP
60 70 80 90 100
RTPVLWLHGL ECTCCSESFI RSAHPLVKDV VLSMISLDYD DTLMAAAGHQ
110 120 130 140 150
AEAALADTIE RYKGNYILAV EGNPPLNEDG MFCIIGGKPF VDQLRYAAKH
160 170 180 190 200
AKAIISWGSC ASHGCVQAAR PNPTRATPVH QVITDKPIIK VPGCPPIAEV
210 220 230 240 250
MTGVITYMLT FGKLPELDRT GRPKMFYSQR IHDKCYRRPH FDAGQFVESF
260 270 280 290 300
DDEGARRGYC LYKVGCKGPT TYNACSTIRW NEGTSFPIQA GHGCIGCSEE
310 320 330 340 350
GFWDKGSWYA RLQDIHQFGI EANADQIGGT VAVGAAGAVA AHAAVSALKR
360
AQTKRQTTTT TTPKEHV
Length:367
Mass (Da):40,129
Last modified:January 25, 2012 - v3
Checksum:iC10AC8FD7690AC50
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62C → E AA sequence (PubMed:9324252).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002827 Genomic DNA. Translation: AEI08138.1.
RefSeqiWP_013913762.1. NC_015689.1.

Genome annotation databases

EnsemblBacteriaiAEI08138; AEI08138; OCA5_pHCG300640.
KEGGiocg:OCA5_pHCG300640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002827 Genomic DNA. Translation: AEI08138.1.
RefSeqiWP_013913762.1. NC_015689.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEI08138; AEI08138; OCA5_pHCG300640.
KEGGiocg:OCA5_pHCG300640.

Phylogenomic databases

KOiK06282.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBHS_OLICO
AccessioniPrimary (citable) accession number: O33405
Secondary accession number(s): F8C128, Q6LB91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: January 25, 2012
Last modified: November 2, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.