Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Uptake hydrogenase small subunit

Gene

hoxS

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.By similarity

Catalytic activityi

H2 + A = AH2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi160 – 1601Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi194 – 1941Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi232 – 2321Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi235 – 2351Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi260 – 2601Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi266 – 2661Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi275 – 2751Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi294 – 2941Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi297 – 2971Iron-sulfur 3 (3Fe-4S)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciOCAR504832:GJPZ-3631-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uptake hydrogenase small subunit (EC:1.12.99.6)
Alternative name(s):
Hydrogenlyase
Membrane-bound hydrogenase small subunit
Gene namesi
Name:hoxS
Ordered Locus Names:OCA5_pHCG300640
Encoded oniPlasmid megaplasmid pHCG30 Publication
OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
Taxonomic identifieri504832 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
Proteomesi
  • UP000007730 Componenti: Plasmid pHCG3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4545Tat-type signalPROSITE-ProRule annotation1 PublicationAdd
BLAST
Chaini46 – 367322Uptake hydrogenase small subunitPRO_0000013433Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK06282.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O33405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPTETFYEV MRRQGVTRRS FLKFCSLTAT ALGLGPAYTS EIAHAMETKP
60 70 80 90 100
RTPVLWLHGL ECTCCSESFI RSAHPLVKDV VLSMISLDYD DTLMAAAGHQ
110 120 130 140 150
AEAALADTIE RYKGNYILAV EGNPPLNEDG MFCIIGGKPF VDQLRYAAKH
160 170 180 190 200
AKAIISWGSC ASHGCVQAAR PNPTRATPVH QVITDKPIIK VPGCPPIAEV
210 220 230 240 250
MTGVITYMLT FGKLPELDRT GRPKMFYSQR IHDKCYRRPH FDAGQFVESF
260 270 280 290 300
DDEGARRGYC LYKVGCKGPT TYNACSTIRW NEGTSFPIQA GHGCIGCSEE
310 320 330 340 350
GFWDKGSWYA RLQDIHQFGI EANADQIGGT VAVGAAGAVA AHAAVSALKR
360
AQTKRQTTTT TTPKEHV
Length:367
Mass (Da):40,129
Last modified:January 25, 2012 - v3
Checksum:iC10AC8FD7690AC50
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621C → E AA sequence (PubMed:9324252).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002827 Genomic DNA. Translation: AEI08138.1.
RefSeqiWP_013913762.1. NC_015689.1.

Genome annotation databases

EnsemblBacteriaiAEI08138; AEI08138; OCA5_pHCG300640.
KEGGiocg:OCA5_pHCG300640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002827 Genomic DNA. Translation: AEI08138.1.
RefSeqiWP_013913762.1. NC_015689.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEI08138; AEI08138; OCA5_pHCG300640.
KEGGiocg:OCA5_pHCG300640.

Phylogenomic databases

KOiK06282.

Enzyme and pathway databases

BioCyciOCAR504832:GJPZ-3631-MONOMER.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and molecular characterization of the H2 uptake membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium Oligotropha carboxidovorans."
    Santiago B., Meyer O.
    J. Bacteriol. 179:6053-6060(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-63.
    Strain: ATCC 49405 / DSM 1227 / OM5.
  2. "Complete nucleotide sequence of the circular megaplasmid pHCG3 of Oligotropha carboxidovorans: function in the chemolithoautotrophic utilization of CO, H(2) and CO(2)."
    Fuhrmann S., Ferner M., Jeffke T., Henne A., Gottschalk G., Meyer O.
    Gene 322:67-75(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49405 / DSM 1227 / OM5.
  3. "Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
    Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
    J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: ATCC 49405 / DSM 1227 / OM5.

Entry informationi

Entry nameiMBHS_OLICO
AccessioniPrimary (citable) accession number: O33405
Secondary accession number(s): F8C128, Q6LB91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: January 25, 2012
Last modified: July 22, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.