O33380 (TYSY_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:

thyA

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Sequence caution	The sequence AAC45469.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000140992
Sites
	Active site	147	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33380 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 135961EEFAFA3FF8	FASTA	265	30,306
	10 20 30 40 50 60 MKAYLDLMRH VLDNGTDKSD RTGTGTLSVF GYQMRFDLGK GFPLLTTKKL HLRSIIHELL 70 80 90 100 110 120 WFLKGDTNIK YLKDNNVSIW DDEWADENGD LGPVYGYQWR SWPAPDGRHI DQIANVVEQI 130 140 150 160 170 180 KKNPDSRRLI VSAWNPALVD EMALPPCHAL FQFYVADGNL SCQLYQRSAD IFLGVPFNIA 190 200 210 220 230 240 SYALLTMMMA QVCGLEAGEF VHTFGDAHLY RNHFEQAALQ LEREPRALPV MKINPEVKDL 250 260 FAFKFEDFEL EGYDPHPHIK AVVSV

References

[1]	"Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae." Carlson J.H., Hill S.A. FEMS Microbiol. Lett. 151:225-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MS11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U86637 Genomic DNA. Translation: AAC45469.1. Different initiation.
3D structure databases
ProteinModelPortal	O33380.
SMR	O33380. Positions 1-265.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_NEIGO

Accession

Primary (citable) accession number: O33380

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 2, 2002
Last modified:	April 3, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program