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Protein

Epoxide hydrolase EphG

Gene

ephG

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Epoxide hydrolase capable of hydrolyzing long or bulky lipophilic epoxides such as 9,10-epoxystearic acid and cholesterol 5,6-oxide in vitro. The physiological substrates have yet to be identified, but could be fatty acid or steroid derivatives.1 Publication

Catalytic activityi

An epoxide + H2O = a glycol.1 Publication
5,6-alpha-epoxy-5-alpha-cholestan-3-beta-ol + H2O = cholestane-3-beta,5-alpha,6-beta-triol.1 Publication
5,6-beta-epoxy-5-beta-cholestan-3-beta-ol + H2O = cholestane-3-beta,5-alpha,6-beta-triol.1 Publication

Enzyme regulationi

Is inhibited by the anti-epileptic drug valpromide (Ki value of about 100 µM).1 Publication

Kineticsi

  1. KM=45 µM for cis-9,10-epoxystearate1 Publication
  2. KM=60 µM for cholesterol 5,6-oxide1 Publication
  1. Vmax=30 nmol/min/mg enzyme with cis-9,10-epoxystearate as substrate1 Publication
  2. Vmax=1.1 nmol/min/mg enzyme with cholesterol 5,6-oxide as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931Proton donor1 Publication
Active sitei122 – 1221Proton acceptor1 Publication

GO - Molecular functioni

  • cholesterol-5,6-oxide hydrolase activity Source: MTBBASE
  • epoxide hydrolase activity Source: MTBBASE

GO - Biological processi

  • epoxide metabolic process Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMTBRV:RV2740-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxide hydrolase EphG (EC:3.3.2.10)
Short name:
EH
Alternative name(s):
Cholesterol-5,6-oxide hydrolase (EC:3.3.2.11)
Gene namesi
Name:ephG
Ordered Locus Names:Rv2740
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2740.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Epoxide hydrolase EphGPRO_0000420415Add
BLAST

Proteomic databases

PaxDbiO33283.
PRIDEiO33283.

Interactioni

Subunit structurei

Homodimer. Is also present as monomer in solution.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv2740.

Structurei

Secondary structure

1
149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 3017Combined sources
Helixi33 – 397Combined sources
Beta strandi40 – 489Combined sources
Turni49 – 513Combined sources
Beta strandi52 – 554Combined sources
Helixi57 – 659Combined sources
Turni66 – 705Combined sources
Beta strandi72 – 8312Combined sources
Beta strandi86 – 9712Combined sources
Beta strandi100 – 11314Combined sources
Beta strandi116 – 1238Combined sources
Helixi126 – 13914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BNGX-ray2.50A/B/C1-149[»]
ProteinModelPortaliO33283.
SMRiO33283. Positions 10-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105MXB. Bacteria.
COG4308. LUCA.
HOGENOMiHOG000219335.
KOiK10533.
OMAiVGLPTIH.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR013100. LEH.
IPR032710. NTF2-like_dom.
[Graphical view]
PfamiPF07858. LEH. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.

Sequencei

Sequence statusi: Complete.

O33283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELTETSPE TPETTEAIRA VEAFLNALQN EDFDTVDAAL GDDLVYENVG
60 70 80 90 100
FSRIRGGRRT ATLLRRMQGR VGFEVKIHRI GADGAAVLTE RTDALIIGPL
110 120 130 140
RVQFWVCGVF EVDDGRITLW RDYFDVYDMF KGLLRGLVAL VVPSLKATL
Length:149
Mass (Da):16,593
Last modified:January 1, 1998 - v1
Checksum:i6941EA603B942822
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45539.1.
PIRiC70878.
RefSeqiNP_217256.1. NC_000962.3.
WP_003414015.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP45539; CCP45539; Rv2740.
GeneIDi888365.
KEGGimtu:Rv2740.
PATRICi18154677. VBIMycTub87468_3052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45539.1.
PIRiC70878.
RefSeqiNP_217256.1. NC_000962.3.
WP_003414015.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BNGX-ray2.50A/B/C1-149[»]
ProteinModelPortaliO33283.
SMRiO33283. Positions 10-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2740.

Proteomic databases

PaxDbiO33283.
PRIDEiO33283.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45539; CCP45539; Rv2740.
GeneIDi888365.
KEGGimtu:Rv2740.
PATRICi18154677. VBIMycTub87468_3052.

Organism-specific databases

TubercuListiRv2740.

Phylogenomic databases

eggNOGiENOG4105MXB. Bacteria.
COG4308. LUCA.
HOGENOMiHOG000219335.
KOiK10533.
OMAiVGLPTIH.

Enzyme and pathway databases

BioCyciMTBRV:RV2740-MONOMER.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR013100. LEH.
IPR032710. NTF2-like_dom.
[Graphical view]
PfamiPF07858. LEH. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEPHG_MYCTU
AccessioniPrimary (citable) accession number: O33283
Secondary accession number(s): F2GPI3, L0TC43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.