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O33174 (HPRT_METTM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hypoxanthine/guanine phosphoribosyltransferase

Short name=HGPRTase
EC=2.4.2.8
Alternative name(s):
Hypoxanthine (guanine) phosphoribosyltransferase
Gene names
Name:hpt
Ordered Locus Names:MTBMA_c17060
OrganismMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]
Taxonomic identifier79929 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis. Prefers hypoxanthine, has 66% activity with guanine while activity with adenine, xanthine, uracil, orotate, or cytosine is negligible. HAMAP-Rule MF_01467

Catalytic activity

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01467

GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01467

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1. HAMAP-Rule MF_01467

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01467

Subcellular location

Cytoplasm HAMAP-Rule MF_01467.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.6-8.2. Ref.1

Temperature dependence:

Optimum temperature is 70-80 degrees Celsius.

Ontologies

Keywords
   Biological processPurine salvage
   Cellular componentCytoplasm
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processIMP salvage

Inferred from electronic annotation. Source: UniProtKB-UniPathway

hypoxanthine salvage

Traceable author statement Ref.1. Source: UniProtKB

purine ribonucleoside salvage

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionguanine phosphoribosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

hypoxanthine phosphoribosyltransferase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193Hypoxanthine/guanine phosphoribosyltransferase HAMAP-Rule MF_01467
PRO_0000149500

Sequences

Sequence LengthMass (Da)Tools
O33174 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: D2377AC07A276322

FASTA19321,219
        10         20         30         40         50         60 
MLDKLKESLR NSPVIKKGEY DYFVNPVTDG IPLTEPELLE EIADEIVRRF NPDPASVDKI 

        70         80         90        100        110        120 
VCIEAMGIHH ATVLSLKTRI PFVVVRKRRY GLPGEVAVHQ MTGYSEGELY INGVDGDDRV 

       130        140        150        160        170        180 
MVIDDVVSTG GTLLAVLEAL REMEVEVVDV VTVIDKGEGS RVVKERTGFT VRSLVKADVV 

       190 
DGRVTVEDIP DGG 

« Hide

References

« Hide 'large scale' references
[1]"Expression of the Methanobacterium thermoautotrophicum hpt gene, encoding hypoxanthine (Guanine) phosphoribosyltransferase, in Escherichia coli."
Sauer J., Nygaard P.
J. Bacteriol. 181:1958-1962(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION AS AN HGPRTASE.
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF007759 Genomic DNA. Translation: AAB62272.1.
CP001710 Genomic DNA. Translation: ADL59275.1.
RefSeqYP_003850588.1. NC_014408.1.

3D structure databases

ProteinModelPortalO33174.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL59275; ADL59275; MTBMA_c17060.
GeneID9705417.
KEGGmmg:MTBMA_c17060.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000228520.
KOK00759.
OMAIATDIPY.

Enzyme and pathway databases

BioCycMMAR79929:GH5J-1712-MONOMER.
UniPathwayUPA00591; UER00648.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
HAMAPMF_01467. Hypx_phosphoribosyltr.
InterProIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHPRT_METTM
AccessionPrimary (citable) accession number: O33174
Secondary accession number(s): D9PYH7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways