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O33174

- HPRT_METTM

UniProt

O33174 - HPRT_METTM

Protein

Hypoxanthine/guanine phosphoribosyltransferase

Gene

hpt

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis. Prefers hypoxanthine, has 66% activity with guanine while activity with adenine, xanthine, uracil, orotate, or cytosine is negligible.

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    pH dependencei

    Optimum pH is 7.6-8.2.1 Publication

    Temperature dependencei

    Optimum temperature is 70-80 degrees Celsius.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. hypoxanthine salvage Source: UniProtKB
    2. IMP salvage Source: UniProtKB-UniPathway
    3. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciMMAR79929:GH5J-1712-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine/guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRTase
    Alternative name(s):
    Hypoxanthine (guanine) phosphoribosyltransferase
    Gene namesi
    Name:hpt
    Ordered Locus Names:MTBMA_c17060
    OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri79929 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000000345: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 193193Hypoxanthine/guanine phosphoribosyltransferasePRO_0000149500Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO33174.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000228520.
    KOiK00759.
    OMAiIATDIPY.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    HAMAPiMF_01467. Hypx_phosphoribosyltr.
    InterProiIPR026597. HGPRTase-like.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O33174-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLDKLKESLR NSPVIKKGEY DYFVNPVTDG IPLTEPELLE EIADEIVRRF    50
    NPDPASVDKI VCIEAMGIHH ATVLSLKTRI PFVVVRKRRY GLPGEVAVHQ 100
    MTGYSEGELY INGVDGDDRV MVIDDVVSTG GTLLAVLEAL REMEVEVVDV 150
    VTVIDKGEGS RVVKERTGFT VRSLVKADVV DGRVTVEDIP DGG 193
    Length:193
    Mass (Da):21,219
    Last modified:January 1, 1998 - v1
    Checksum:iD2377AC07A276322
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007759 Genomic DNA. Translation: AAB62272.1.
    CP001710 Genomic DNA. Translation: ADL59275.1.
    RefSeqiYP_003850588.1. NC_014408.1.

    Genome annotation databases

    EnsemblBacteriaiADL59275; ADL59275; MTBMA_c17060.
    GeneIDi9705417.
    KEGGimmg:MTBMA_c17060.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007759 Genomic DNA. Translation: AAB62272.1 .
    CP001710 Genomic DNA. Translation: ADL59275.1 .
    RefSeqi YP_003850588.1. NC_014408.1.

    3D structure databases

    ProteinModelPortali O33174.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL59275 ; ADL59275 ; MTBMA_c17060 .
    GeneIDi 9705417.
    KEGGi mmg:MTBMA_c17060.

    Phylogenomic databases

    HOGENOMi HOG000228520.
    KOi K00759.
    OMAi IATDIPY.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci MMAR79929:GH5J-1712-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    HAMAPi MF_01467. Hypx_phosphoribosyltr.
    InterProi IPR026597. HGPRTase-like.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of the Methanobacterium thermoautotrophicum hpt gene, encoding hypoxanthine (Guanine) phosphoribosyltransferase, in Escherichia coli."
      Sauer J., Nygaard P.
      J. Bacteriol. 181:1958-1962(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION AS AN HGPRTASE.
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
    2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
      Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
      J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

    Entry informationi

    Entry nameiHPRT_METTM
    AccessioniPrimary (citable) accession number: O33174
    Secondary accession number(s): D9PYH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 25, 2003
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3