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O33174

- HPRT_METTM

UniProt

O33174 - HPRT_METTM

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Protein
Hypoxanthine/guanine phosphoribosyltransferase
Gene
hpt, MTBMA_c17060
Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis. Prefers hypoxanthine, has 66% activity with guanine while activity with adenine, xanthine, uracil, orotate, or cytosine is negligible.UniRule annotation

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

pH dependencei

Optimum pH is 7.6-8.2.1 Publication

Temperature dependencei

Optimum temperature is 70-80 degrees Celsius.

Pathwayi

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. hypoxanthine salvage Source: UniProtKB
  3. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1712-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine/guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRTase
Alternative name(s):
Hypoxanthine (guanine) phosphoribosyltransferase
Gene namesi
Name:hpt
Ordered Locus Names:MTBMA_c17060
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Hypoxanthine/guanine phosphoribosyltransferaseUniRule annotation
PRO_0000149500Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO33174.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000228520.
KOiK00759.
OMAiIATDIPY.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01467. Hypx_phosphoribosyltr.
InterProiIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33174-1 [UniParc]FASTAAdd to Basket

« Hide

MLDKLKESLR NSPVIKKGEY DYFVNPVTDG IPLTEPELLE EIADEIVRRF    50
NPDPASVDKI VCIEAMGIHH ATVLSLKTRI PFVVVRKRRY GLPGEVAVHQ 100
MTGYSEGELY INGVDGDDRV MVIDDVVSTG GTLLAVLEAL REMEVEVVDV 150
VTVIDKGEGS RVVKERTGFT VRSLVKADVV DGRVTVEDIP DGG 193
Length:193
Mass (Da):21,219
Last modified:January 1, 1998 - v1
Checksum:iD2377AC07A276322
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF007759 Genomic DNA. Translation: AAB62272.1.
CP001710 Genomic DNA. Translation: ADL59275.1.
RefSeqiYP_003850588.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL59275; ADL59275; MTBMA_c17060.
GeneIDi9705417.
KEGGimmg:MTBMA_c17060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF007759 Genomic DNA. Translation: AAB62272.1 .
CP001710 Genomic DNA. Translation: ADL59275.1 .
RefSeqi YP_003850588.1. NC_014408.1.

3D structure databases

ProteinModelPortali O33174.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADL59275 ; ADL59275 ; MTBMA_c17060 .
GeneIDi 9705417.
KEGGi mmg:MTBMA_c17060.

Phylogenomic databases

HOGENOMi HOG000228520.
KOi K00759.
OMAi IATDIPY.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .
BioCyci MMAR79929:GH5J-1712-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
HAMAPi MF_01467. Hypx_phosphoribosyltr.
InterProi IPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the Methanobacterium thermoautotrophicum hpt gene, encoding hypoxanthine (Guanine) phosphoribosyltransferase, in Escherichia coli."
    Sauer J., Nygaard P.
    J. Bacteriol. 181:1958-1962(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION AS AN HGPRTASE.
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Entry informationi

Entry nameiHPRT_METTM
AccessioniPrimary (citable) accession number: O33174
Secondary accession number(s): D9PYH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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