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Protein

Hypoxanthine/guanine phosphoribosyltransferase

Gene

hpt

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis. Prefers hypoxanthine, has 66% activity with guanine while activity with adenine, xanthine, uracil, orotate, or cytosine is negligible.

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

pH dependencei

Optimum pH is 7.6-8.2.1 Publication

Temperature dependencei

Optimum temperature is 70-80 degrees Celsius.1 Publication

Pathway:iIMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine/guanine phosphoribosyltransferase (hpt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

  • hypoxanthine salvage Source: UniProtKB
  • IMP salvage Source: UniProtKB-UniPathway
  • purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1712-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine/guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRTase
Alternative name(s):
Hypoxanthine (guanine) phosphoribosyltransferase
Gene namesi
Name:hpt
Ordered Locus Names:MTBMA_c17060
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Hypoxanthine/guanine phosphoribosyltransferasePRO_0000149500Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi79929.MTBMA_c17060.

Structurei

3D structure databases

ProteinModelPortaliO33174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000228520.
KOiK00759.
OMAiIATDIPY.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01467. Hypx_phosphoribosyltr.
InterProiIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDKLKESLR NSPVIKKGEY DYFVNPVTDG IPLTEPELLE EIADEIVRRF
60 70 80 90 100
NPDPASVDKI VCIEAMGIHH ATVLSLKTRI PFVVVRKRRY GLPGEVAVHQ
110 120 130 140 150
MTGYSEGELY INGVDGDDRV MVIDDVVSTG GTLLAVLEAL REMEVEVVDV
160 170 180 190
VTVIDKGEGS RVVKERTGFT VRSLVKADVV DGRVTVEDIP DGG
Length:193
Mass (Da):21,219
Last modified:January 1, 1998 - v1
Checksum:iD2377AC07A276322
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007759 Genomic DNA. Translation: AAB62272.1.
CP001710 Genomic DNA. Translation: ADL59275.1.
RefSeqiWP_013296485.1. NC_014408.1.
YP_003850588.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL59275; ADL59275; MTBMA_c17060.
GeneIDi9705417.
KEGGimmg:MTBMA_c17060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007759 Genomic DNA. Translation: AAB62272.1.
CP001710 Genomic DNA. Translation: ADL59275.1.
RefSeqiWP_013296485.1. NC_014408.1.
YP_003850588.1. NC_014408.1.

3D structure databases

ProteinModelPortaliO33174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi79929.MTBMA_c17060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL59275; ADL59275; MTBMA_c17060.
GeneIDi9705417.
KEGGimmg:MTBMA_c17060.

Phylogenomic databases

HOGENOMiHOG000228520.
KOiK00759.
OMAiIATDIPY.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
BioCyciMMAR79929:GH5J-1712-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01467. Hypx_phosphoribosyltr.
InterProiIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the Methanobacterium thermoautotrophicum hpt gene, encoding hypoxanthine (Guanine) phosphoribosyltransferase, in Escherichia coli."
    Sauer J., Nygaard P.
    J. Bacteriol. 181:1958-1962(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION AS AN HGPRTASE.
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Entry informationi

Entry nameiHPRT_METTM
AccessioniPrimary (citable) accession number: O33174
Secondary accession number(s): D9PYH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.