Skip Header

Contribute Send feedback
Read comments (?) or add your own

O33149 (GYRA_MYCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A
EC=5.99.1.3

Cleaved into the following chain:

  1. Mma gyrA intein
Gene names
Name:gyrA
OrganismMycobacterium malmoense
Taxonomic identifier1780 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length473 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01897.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP-Rule MF_01897

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4949DNA gyrase subunit A, 1st part By similarity
PRO_0000034810
Chain50 – 469420Mma gyrA intein By similarity
PRO_0000034811
Chain470 – ›473›4DNA gyrase subunit A, 2nd part By similarity
PRO_0000034812

Regions

Domain175 – 315141DOD-type homing endonuclease

Sites

Active site491O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Non-terminal residue4731

Sequences

Sequence LengthMass (Da)Tools
O33149 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 5DD88B4F50A2AEBC

FASTA47352,436
        10         20         30         40         50         60 
MGNYHPHGNA SIYNTLVRMA QPWSLRYPLV NGQGNFGSPG NNPPAAMRYC CTGDALVRLP 

        70         80         90        100        110        120 
FGHSVRIGNF VPAACPNSDN AVNLKVLDRH GDPVVADQLF HSGEHQTYTV RTAEGYEVTG 

       130        140        150        160        170        180 
TSNHPLLCLV DVGGVPTLLW KLIEEIRPDD HVVLQRTPPV EFGPADWHDV MEALLLGAFI 

       190        200        210        220        230        240 
SEGFVSEVRA GFNNCDRDYF AMVVGAYDAV VGGRRYVSSR RIASGSTLHE LDIQNIKELK 

       250        260        270        280        290        300 
EARLGDLCGQ RPADKSVPDW LWHSPAAVKR VFLQALFEGG GSCSALPRNM IQISYSTRSR 

       310        320        330        340        350        360 
QLAVDVQQML LEFGIITRRY RHAVGEHKVL ITNRAQAELF ATRVGFGGAK QEKLTKILGS 

       370        380        390        400        410        420 
MPPCAGMDSD HVPGLARFIR KHCGSRWVDK DWLNRHNVDR IQRWRTSGEK ILSHIADPDV 

       430        440        450        460        470 
RAIATDLTDG RFYYAKVASV TEAGVQPVYS LRVDTDEHAF LTNGFVSHNT EAR

« Hide

References

[1]"Inteins in mycobacterial GyrA are a taxonomic character."
Sander P., Alcaide F., Richter I., Frischkorn K., Tortoli E., Springer B., Telenti A., Boettger E.C.
Microbiology 144:589-591(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ002066 Genomic DNA. Translation: CAA05167.1.

3D structure databases

ProteinModelPortalO33149.
SMRO33149. Positions 1-49, 52-157, 413-469.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.90.199.10. 1 hit.
HAMAPMF_01897. GyrA.
InterProIPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGYRA_MYCMA
AccessionPrimary (citable) accession number: O33149
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents