Reviewed, UniProtKB/Swiss-Prot O33149 (GYRA_MYCMA)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3
Cleaved into the following chain:
    1- Recommended name:
            Mma gyrA intein

Gene names

Name:

gyrA

Organism

Mycobacterium malmoense

Taxonomic identifier

1780 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	473 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Isomerase Nuclease Topoisomerase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 49	49	DNA gyrase subunit A, 1st part By similarity		PRO_0000034810
	Chain	50 – 469	420	Mma gyrA intein By similarity		PRO_0000034811
	Chain	470 – ›473	›4	DNA gyrase subunit A, 2nd part By similarity		PRO_0000034812
Regions
	Domain	175 – 315	141	DOD-type homing endonuclease
Experimental info
	Non-terminal residue	473	1

Sequences

	Sequence		Length	Mass (Da)
	O33149-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5DD88B4F50A2AEBC	FASTA	473	52,436
	10 20 30 40 50 60 MGNYHPHGNA SIYNTLVRMA QPWSLRYPLV NGQGNFGSPG NNPPAAMRYC CTGDALVRLP 70 80 90 100 110 120 FGHSVRIGNF VPAACPNSDN AVNLKVLDRH GDPVVADQLF HSGEHQTYTV RTAEGYEVTG 130 140 150 160 170 180 TSNHPLLCLV DVGGVPTLLW KLIEEIRPDD HVVLQRTPPV EFGPADWHDV MEALLLGAFI 190 200 210 220 230 240 SEGFVSEVRA GFNNCDRDYF AMVVGAYDAV VGGRRYVSSR RIASGSTLHE LDIQNIKELK 250 260 270 280 290 300 EARLGDLCGQ RPADKSVPDW LWHSPAAVKR VFLQALFEGG GSCSALPRNM IQISYSTRSR 310 320 330 340 350 360 QLAVDVQQML LEFGIITRRY RHAVGEHKVL ITNRAQAELF ATRVGFGGAK QEKLTKILGS 370 380 390 400 410 420 MPPCAGMDSD HVPGLARFIR KHCGSRWVDK DWLNRHNVDR IQRWRTSGEK ILSHIADPDV 430 440 450 460 470 RAIATDLTDG RFYYAKVASV TEAGVQPVYS LRVDTDEHAF LTNGFVSHNT EAR

References

[1]	"Inteins in mycobacterial GyrA are a taxonomic character." Sander P., Alcaide F., Richter I., Frischkorn K., Tortoli E., Springer B., Telenti A., Boettger E.C. Microbiology 144:589-591(1998) [PubMed: 9493395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ002066 Genomic DNA. Translation: CAA05167.1.
3D structure databases
SMR	O33149. Positions 1-132, 50-469.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.3. 295882.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR002205. Topo_IIA_A/C. IPR013760. Topo_IIA_cen. [Graphical view]
Pfam	PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCMA

Accession

Primary (citable) accession number: O33149

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)