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Protein

3-isopropylmalate dehydratase small subunit

Gene

leuD

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.By similarity

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Probable branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase small subunit (EC:4.2.1.33)
Alternative name(s):
Alpha-IPM isomerase
Short name:
IPMI
Isopropylmalate isomerase
Gene namesi
Name:leuD
Ordered Locus Names:ML1684
ORF Names:MLCB637.33
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1684.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1981983-isopropylmalate dehydratase small subunitPRO_0000141838Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.By similarity

Protein-protein interaction databases

STRINGi272631.ML1684.

Structurei

3D structure databases

ProteinModelPortaliO33124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LeuD family. LeuD type 1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0066. LUCA.
HOGENOMiHOG000222939.
KOiK01704.
OMAiAFTTHTG.
OrthoDBiEOG661HCP.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.

Sequencei

Sequence statusi: Complete.

O33124-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAFRVHTGI GVPLRRSNVD TDQIIPAVFL KRVTRNGFED GLFATWRVDP
60 70 80 90 100
SFVLNLSPFD RGSVLVVGPD FGIGSSREHA VWALMDYGFR VVISSRFGDI
110 120 130 140 150
FHGNAGKAGL LAAQVAQDDV EFLWKLIEQK PGLEITVNLQ DRNINAATVV
160 170 180 190
LPFKIDDYTA WRLLEGLDDI ALNLRKLDEI EAFESARPAW KPRTLPTT
Length:198
Mass (Da):22,136
Last modified:January 1, 1998 - v1
Checksum:i25C1CD4E7FF089FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99263 Genomic DNA. Translation: CAB16448.1.
AL583923 Genomic DNA. Translation: CAC30637.1.
PIRiT45426.
RefSeqiNP_302158.1. NC_002677.1.
WP_010908479.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30637; CAC30637; CAC30637.
GeneIDi910051.
KEGGimle:ML1684.
PATRICi18056490. VBIMycLep78757_3176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99263 Genomic DNA. Translation: CAB16448.1.
AL583923 Genomic DNA. Translation: CAC30637.1.
PIRiT45426.
RefSeqiNP_302158.1. NC_002677.1.
WP_010908479.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO33124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30637; CAC30637; CAC30637.
GeneIDi910051.
KEGGimle:ML1684.
PATRICi18056490. VBIMycLep78757_3176.

Organism-specific databases

LepromaiML1684.

Phylogenomic databases

eggNOGiCOG0066. LUCA.
HOGENOMiHOG000222939.
KOiK01704.
OMAiAFTTHTG.
OrthoDBiEOG661HCP.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiLEUD_MYCLE
AccessioniPrimary (citable) accession number: O33124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.