O33116 (SERA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-3-phosphoglycerate dehydrogenase
Short name=PGDH
EC=1.1.1.95

Gene names

Name:	serA
Ordered Locus Names:	ML1692
ORF Names:	MLCB637.25

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	D-3-phosphoglycerate dehydrogenase		PRO_0000076004
Regions
	Domain	454 – 525	72	ACT
	Nucleotide binding	151 – 152	2	NAD By similarity
	Nucleotide binding	230 – 232	3	NAD By similarity
	Nucleotide binding	279 – 282	4	NAD By similarity
Sites
	Active site	232	1	By similarity
	Active site	261	1	By similarity
	Active site	279	1	Proton donor By similarity
	Binding site	171	1	NAD By similarity
	Binding site	256	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33116 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1A6DC60F9FB71222	FASTA	528	54,469
	10 20 30 40 50 60 MDLPVVLIAD KLAQSTVAAL GDQVEVRWVD GPDRTKLLAA VPEADALLVR SATTVDAEVL 70 80 90 100 110 120 AAAPKLKIVA RAGVGLDNVD VDAATARGVL VVNAPTSNIH SAAEHALALL LAASRQIAEA 130 140 150 160 170 180 DASLRAHIWK RSSFSGTEIF GKTVGVVGLG RIGQLVAARI AAFGAHVIAY DPYVAPARAA 190 200 210 220 230 240 QLGIELMSFD DLLARADFIS VHLPKTPETA GLIDKEALAK TKPGVIIVNA ARGGLVDEVA 250 260 270 280 290 300 LADAVRSGHV RAAGLDVFAT EPCTDSPLFE LSQVVVTPHL GASTAEAQDR AGTDVAESVR 310 320 330 340 350 360 LALAGEFVPD AVNVDGGVVN EEVAPWLDLV CKLGVLVAAL SDELPASLSV HVRGELASED 370 380 390 400 410 420 VEILRLSALR GLFSTVIEDA VTFVNAPALA AERGVSAEIT TGSESPNHRS VVDVRAVASD 430 440 450 460 470 480 GSVVNIAGTL SGPQLVQKIV QVNGRNFDLR AQGMNLVIRY VDQPGALGKI GTLLGAAGVN 490 500 510 520 IQAAQLSEDT EGPGATILLR LDQDVPGDVR SAIVAAVSAN KLEVVNLS

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z99263 Genomic DNA. Translation: CAB16440.1. AL583923 Genomic DNA. Translation: CAC30645.1.
PIR	T45418.
RefSeq	NP_302163.1. NC_002677.1.
3D structure databases
ProteinModelPortal	O33116.
SMR	O33116. Positions 3-528.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029282; EBMYCP00000028888; EBMYCG00000029277.
GeneID	910062.
GenomeReviews	Gene locus ML1692 in contig AL450380_GR.
KEGG	mle:ML1692.
NMPDR	fig|272631.1.peg.1035.
PATRIC	18056520. VBIMycLep78757_3189.
Organism-specific databases
Leproma	ML1692.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016363.
HOGENOM	HBG731446.
OMA	AEHTVAM.
ProtClustDB	PRK13581.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1692-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR006236. D-3-Phosphoglycerate_DH. IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K00058.
PANTHER	PTHR10996:SF20. D3PG_Deh. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF01842. ACT. 1 hit. [Graphical view]
TIGRFAMs	TIGR01327. PGDH. 1 hit.
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_MYCLE

Accession

Primary (citable) accession number: O33116

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program