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Protein

Acetolactate synthase

Gene

ilvB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit (ilvH), Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Probable branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit (ilvH), Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Probable branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921Thiamine pyrophosphateBy similarity
Binding sitei194 – 1941FADBy similarity
Metal bindingi487 – 4871MagnesiumBy similarity
Metal bindingi514 – 5141MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi300 – 32122FADBy similarityAdd
BLAST
Nucleotide bindingi343 – 36220FADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase (EC:2.2.1.6)
Alternative name(s):
ALS
Acetohydroxy-acid synthase
Gene namesi
Name:ilvB
Ordered Locus Names:ML1696
ORF Names:MLCB637.20
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1696.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625Acetolactate synthasePRO_0000090802Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML1696.

Structurei

3D structure databases

ProteinModelPortaliO33112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni436 – 51681Thiamine pyrophosphate bindingAdd
BLAST

Sequence similaritiesi

Belongs to the TPP enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105C7K. Bacteria.
COG0028. LUCA.
HOGENOMiHOG000258448.
KOiK01652.
OMAiMVWPMVP.
OrthoDBiEOG6KT2NW.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPTKPHAR PQGAGNSVPN TVKPATQFPS KPAVAKLERV SPEQLTGAQS
60 70 80 90 100
VIRSLEELDV EVIFGIPGGA VLLVYDPLFY SKKLRHVLVR HEQGAGHAAS
110 120 130 140 150
GYAHVTGKVG VCMATSGPGA TNLVTPLADA QMDSVPVVAI TGQVGRSLIG
160 170 180 190 200
TDAFQEADIS GITMPITKHN FLVRAGDDIP RVLAEAFHIA SSGRPGAVLV
210 220 230 240 250
DIPKDVLQGQ CKFSWPPKMD LPGYKPNTKP HNRQIRAAAK LIADARKPVL
260 270 280 290 300
YVGGGVIRGE ATEQLRDLAE LTGIPVVSTL MARGAFPDSH HQNLGMPGMH
310 320 330 340 350
GTVAAVAALQ RSDLLIALGT RFDDRVTGKL DSFAPDAKVI HADIDPAEIG
360 370 380 390 400
KNRHADVPIV GDVKAVIVEL IAMLRHYEVP GNIEMTDWWS YLDGVRKTYP
410 420 430 440 450
LSYSPQSDGT LSPEYVIEKL GEIVGPEAVY VAGVGQHQMW AAQFISYEKP
460 470 480 490 500
RTWLNSGGLG TMGFAIPAAM GAKIARPEAE VWAIDGDGCF QMTNQELATC
510 520 530 540 550
AIEGAPIKVA LINNGNLGMV RQWQALFYQE RYSQTDLATH SHRIPDFVKL
560 570 580 590 600
AEALGCVGLR CECEEDVVDV INQARAINNR PVVIDFIVGA DAQVWPMVAA
610 620
GASNDEIQAA RGIRPLFDDE SEGHV
Length:625
Mass (Da):67,021
Last modified:January 1, 1998 - v1
Checksum:i53FBA3DA76A169D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99263 Genomic DNA. Translation: CAB16435.1.
AL583923 Genomic DNA. Translation: CAC30649.1.
PIRiT45413.
RefSeqiNP_302166.1. NC_002677.1.
WP_010908487.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30649; CAC30649; CAC30649.
GeneIDi910798.
KEGGimle:ML1696.
PATRICi18056540. VBIMycLep78757_3199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99263 Genomic DNA. Translation: CAB16435.1.
AL583923 Genomic DNA. Translation: CAC30649.1.
PIRiT45413.
RefSeqiNP_302166.1. NC_002677.1.
WP_010908487.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO33112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30649; CAC30649; CAC30649.
GeneIDi910798.
KEGGimle:ML1696.
PATRICi18056540. VBIMycLep78757_3199.

Organism-specific databases

LepromaiML1696.

Phylogenomic databases

eggNOGiENOG4105C7K. Bacteria.
COG0028. LUCA.
HOGENOMiHOG000258448.
KOiK01652.
OMAiMVWPMVP.
OrthoDBiEOG6KT2NW.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiILVB_MYCLE
AccessioniPrimary (citable) accession number: O33112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.