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O33106

- PFKA_MYCLE

UniProt

O33106 - PFKA_MYCLE

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
Sitei105 – 1051Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei128 – 1281Proton acceptorUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Binding sitei267 – 2671Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi103 – 1064ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:ML1701
ORF Names:MLCB637.14
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML1701.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343ATP-dependent 6-phosphofructokinasePRO_0000111967Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1701.

Structurei

3D structure databases

ProteinModelPortaliO33106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingUniRule annotation
Regioni170 – 1723Substrate bindingUniRule annotation
Regioni273 – 2764Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiNEICKAM.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O33106-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIGILTGGG DCPGLNAVIR AIVRTCDARY GSSVVGFQDG WRGLLENRRM
60 70 80 90 100
QLCNDDRNDR LLAKGGTMLG TAHVHPDKLR AGLHQIKQTL DDNGIDVLIP
110 120 130 140 150
IGGEGTLTAA HWLSQEDVPV VGVPKTIDND IDCTDVTFGH DTALTVATEA
160 170 180 190 200
IDRLHSTAES HQRVMLVEVM GRHAGWIALS SGLASGAHMT LIPEQPFDVE
210 220 230 240 250
EVCCLVKRRF QRGDSHFICV VAEGAKPVPG SITLRQGGMD EFGHERFTGV
260 270 280 290 300
AAQLGAEVEK RINKDVRVTV LGHVQRGGTP TAFDRVLATR FGVNAADASH
310 320 330 340
AGEYGQMVSL RGQDIGRVPL EDAVRQLKLV PESRYDDAAA FFG
Length:343
Mass (Da):37,002
Last modified:April 27, 2001 - v2
Checksum:i7437B8F5C2318227
GO

Sequence cautioni

The sequence CAB16429.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99263 Genomic DNA. Translation: CAB16429.1. Different initiation.
AL583923 Genomic DNA. Translation: CAC30654.1.
PIRiG87121.
T45407.
RefSeqiNP_302170.1. NC_002677.1.
WP_010908491.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30654; CAC30654; CAC30654.
GeneIDi910793.
KEGGimle:ML1701.
PATRICi18056560. VBIMycLep78757_3209.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99263 Genomic DNA. Translation: CAB16429.1 . Different initiation.
AL583923 Genomic DNA. Translation: CAC30654.1 .
PIRi G87121.
T45407.
RefSeqi NP_302170.1. NC_002677.1.
WP_010908491.1. NC_002677.1.

3D structure databases

ProteinModelPortali O33106.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML1701.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC30654 ; CAC30654 ; CAC30654 .
GeneIDi 910793.
KEGGi mle:ML1701.
PATRICi 18056560. VBIMycLep78757_3209.

Organism-specific databases

Lepromai ML1701.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi NEICKAM.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiPFKA_MYCLE
AccessioniPrimary (citable) accession number: O33106
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 27, 2001
Last modified: November 26, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3