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O33106 (PFKA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:ML1701
ORF Names:MLCB637.14
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Sequence caution

The sequence CAB16429.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_01976
PRO_0000111967

Regions

Nucleotide binding103 – 1064ATP By similarity
Region126 – 1283Substrate binding By similarity
Region170 – 1723Substrate binding By similarity
Region273 – 2764Substrate binding By similarity

Sites

Active site1281Proton acceptor By similarity
Metal binding1041Magnesium; catalytic By similarity
Binding site101ATP; via amide nitrogen By similarity
Binding site1631Substrate; shared with dimeric partner By similarity
Binding site2231Substrate By similarity
Binding site2671Substrate; shared with dimeric partner By similarity
Site1051Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
O33106 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 7437B8F5C2318227

FASTA34337,002
        10         20         30         40         50         60 
MRIGILTGGG DCPGLNAVIR AIVRTCDARY GSSVVGFQDG WRGLLENRRM QLCNDDRNDR 

        70         80         90        100        110        120 
LLAKGGTMLG TAHVHPDKLR AGLHQIKQTL DDNGIDVLIP IGGEGTLTAA HWLSQEDVPV 

       130        140        150        160        170        180 
VGVPKTIDND IDCTDVTFGH DTALTVATEA IDRLHSTAES HQRVMLVEVM GRHAGWIALS 

       190        200        210        220        230        240 
SGLASGAHMT LIPEQPFDVE EVCCLVKRRF QRGDSHFICV VAEGAKPVPG SITLRQGGMD 

       250        260        270        280        290        300 
EFGHERFTGV AAQLGAEVEK RINKDVRVTV LGHVQRGGTP TAFDRVLATR FGVNAADASH 

       310        320        330        340 
AGEYGQMVSL RGQDIGRVPL EDAVRQLKLV PESRYDDAAA FFG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z99263 Genomic DNA. Translation: CAB16429.1. Different initiation.
AL583923 Genomic DNA. Translation: CAC30654.1.
PIRG87121.
T45407.
RefSeqNP_302170.1. NC_002677.1.

3D structure databases

ProteinModelPortalO33106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML1701.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC30654; CAC30654; CAC30654.
GeneID910793.
KEGGmle:ML1701.
PATRIC18056560. VBIMycLep78757_3209.

Organism-specific databases

LepromaML1701.
CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248869.
KOK00850.
OMANEICKAM.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02483. PFK_mixed. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_MYCLE
AccessionPrimary (citable) accession number: O33106
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways