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O33106

- PFKA_MYCLE

UniProt

O33106 - PFKA_MYCLE

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (27 Apr 2001)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
    Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
    Sitei105 – 1051Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei128 – 1281Proton acceptorUniRule annotation
    Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei223 – 2231SubstrateUniRule annotation
    Binding sitei267 – 2671Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi103 – 1064ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:ML1701
    ORF Names:MLCB637.14
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML1701.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343ATP-dependent 6-phosphofructokinasePRO_0000111967Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272631.ML1701.

    Structurei

    3D structure databases

    ProteinModelPortaliO33106.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni170 – 1723Substrate bindingUniRule annotation
    Regioni273 – 2764Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiNEICKAM.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O33106-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIGILTGGG DCPGLNAVIR AIVRTCDARY GSSVVGFQDG WRGLLENRRM    50
    QLCNDDRNDR LLAKGGTMLG TAHVHPDKLR AGLHQIKQTL DDNGIDVLIP 100
    IGGEGTLTAA HWLSQEDVPV VGVPKTIDND IDCTDVTFGH DTALTVATEA 150
    IDRLHSTAES HQRVMLVEVM GRHAGWIALS SGLASGAHMT LIPEQPFDVE 200
    EVCCLVKRRF QRGDSHFICV VAEGAKPVPG SITLRQGGMD EFGHERFTGV 250
    AAQLGAEVEK RINKDVRVTV LGHVQRGGTP TAFDRVLATR FGVNAADASH 300
    AGEYGQMVSL RGQDIGRVPL EDAVRQLKLV PESRYDDAAA FFG 343
    Length:343
    Mass (Da):37,002
    Last modified:April 27, 2001 - v2
    Checksum:i7437B8F5C2318227
    GO

    Sequence cautioni

    The sequence CAB16429.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z99263 Genomic DNA. Translation: CAB16429.1. Different initiation.
    AL583923 Genomic DNA. Translation: CAC30654.1.
    PIRiG87121.
    T45407.
    RefSeqiNP_302170.1. NC_002677.1.
    WP_010908491.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC30654; CAC30654; CAC30654.
    GeneIDi910793.
    KEGGimle:ML1701.
    PATRICi18056560. VBIMycLep78757_3209.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z99263 Genomic DNA. Translation: CAB16429.1 . Different initiation.
    AL583923 Genomic DNA. Translation: CAC30654.1 .
    PIRi G87121.
    T45407.
    RefSeqi NP_302170.1. NC_002677.1.
    WP_010908491.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali O33106.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML1701.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC30654 ; CAC30654 ; CAC30654 .
    GeneIDi 910793.
    KEGGi mle:ML1701.
    PATRICi 18056560. VBIMycLep78757_3209.

    Organism-specific databases

    Lepromai ML1701.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi NEICKAM.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiPFKA_MYCLE
    AccessioniPrimary (citable) accession number: O33106
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3