O33106 (K6PF_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	ML1701
ORF Names:	MLCB637.14

Organism

Mycobacterium leprae (strain TN) [Complete proteome] [HAMAP]

Taxonomic identifier

272631 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.
Sequence caution	The sequence CAB16429.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_0000111967
Regions
	Nucleotide binding	20 – 24	5	ATP By similarity
	Nucleotide binding	155 – 159	5	ATP By similarity
	Nucleotide binding	172 – 188	17	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Metal binding	186	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	267	1	Substrate By similarity
	Binding site	273	1	Substrate By similarity
	Binding site	276	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33106 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 7437B8F5C2318227	FASTA	343	37,002
	10 20 30 40 50 60 MRIGILTGGG DCPGLNAVIR AIVRTCDARY GSSVVGFQDG WRGLLENRRM QLCNDDRNDR 70 80 90 100 110 120 LLAKGGTMLG TAHVHPDKLR AGLHQIKQTL DDNGIDVLIP IGGEGTLTAA HWLSQEDVPV 130 140 150 160 170 180 VGVPKTIDND IDCTDVTFGH DTALTVATEA IDRLHSTAES HQRVMLVEVM GRHAGWIALS 190 200 210 220 230 240 SGLASGAHMT LIPEQPFDVE EVCCLVKRRF QRGDSHFICV VAEGAKPVPG SITLRQGGMD 250 260 270 280 290 300 EFGHERFTGV AAQLGAEVEK RINKDVRVTV LGHVQRGGTP TAFDRVLATR FGVNAADASH 310 320 330 340 AGEYGQMVSL RGQDIGRVPL EDAVRQLKLV PESRYDDAAA FFG

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z99263 Genomic DNA. Translation: CAB16429.1. Different initiation. AL583923 Genomic DNA. Translation: CAC30654.1.
PIR	G87121. T45407.
RefSeq	NP_302170.1. NC_002677.1.
3D structure databases
ProteinModelPortal	O33106.
ModBase	Search...
Protein-protein interaction databases
STRING	272631.ML1701.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC30654; CAC30654; CAC30654.
GeneID	910793.
KEGG	mle:ML1701.
PATRIC	18056560. VBIMycLep78757_3209.
Organism-specific databases
Leproma	ML1701.
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248869.
KO	K00850.
OMA	SAQANIC.
ProtClustDB	PRK03202.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012829. PFK. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02483. PFK_mixed. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_MYCLE

Accession

Primary (citable) accession number: O33106

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program