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O33102 (DNLJ_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Synonyms:lig
Ordered Locus Names:ML1705
ORF Names:MLCB637.10
OrganismMycobacterium leprae [Complete proteome] [HAMAP]
Taxonomic identifier1769 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length694 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 694694DNA ligase HAMAP MF_01588
PRO_0000161751

Regions

Domain607 – 69488BRCT
Nucleotide binding41 – 455NAD By similarity
Nucleotide binding91 – 922NAD By similarity

Sites

Active site1231N6-AMP-lysine intermediate By similarity
Metal binding4181Zinc By similarity
Metal binding4211Zinc By similarity
Metal binding4371Zinc By similarity
Metal binding4431Zinc By similarity
Binding site1211NAD By similarity
Binding site1441NAD By similarity
Binding site1841NAD By similarity
Binding site3001NAD By similarity
Binding site3241NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
O33102 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: CFE712B6AFDAA9D5

FASTA69475,912
        10         20         30         40         50         60 
MSSPEADPIG PEVLQQWRKL TEEVREHQFR YYVRDAPIIS DAEFDALLDR LTVLEEQHPE 

        70         80         90        100        110        120 
LCTPDSPTQL VGGAGFMTDF VATEHLERMF SLDNAFSGDE LNAWVTRIRA EIGNDAHYLC 

       130        140        150        160        170        180 
ELKIDGVALS LVYRDGRLTR ATTRGDGRTG EDVTLNARTI EDVPERLTVS DIYPLPEVLE 

       190        200        210        220        230        240 
VRGEVFFRVV DFQALNTSLV EEGKNPFANP RNSAAGSLRQ KDPAVTAHRK LRMICHGLGH 

       250        260        270        280        290        300 
IEGFRPATQH QAYLALRDWG LPVSEHTTLV NDIAGVQGCI AYWAEHRHEV DHEIDGIVVK 

       310        320        330        340        350        360 
IDEVTLQRRL GSTSRAPRWA IAYKYLPEEA QTKLLDIRVN VGRTGRVTPF AFMTPVKVAG 

       370        380        390        400        410        420 
STVGQATLHN PSEVKRKGVL IGDTVVIRKA GDVIPEVLGP VVDLRDGSER EFVMPTTCPE 

       430        440        450        460        470        480 
CGTTLAPEKE GDADIRCPNA RSCPGQLRER VFHVASRSAL DIQGLGYEAG VALLAAQVIT 

       490        500        510        520        530        540 
SEGDLFTLTE KALLRTELFR NKAGELSANG KRLLVNVDKA KTAPLWRVLV ALSIRHVGPT 

       550        560        570        580        590        600 
AARALATEFG SVDAILAASP EQLAAVEGVG TTIAAAVTEW FTVDWHRVIV NKWRAAGVRM 

       610        620        630        640        650        660 
VDERDTSVLP TCEGLTIVVT GSLAGFSRDD AKEAIVARGG KVAGSVSKKI AYVVVGDLPG 

       670        680        690 
YKYDKAVELG VPILNEDGFR LLLEQGPPVQ QVVD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z99263 Genomic DNA. Translation: CAB16425.1.
AL583923 Genomic DNA. Translation: CAC30658.1.
PIRT45403.
RefSeqNP_302174.1. NC_002677.1.

3D structure databases

ProteinModelPortalO33102.
SMRO33102. Positions 10-328.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000028494; EBMYCP00000028100; EBMYCG00000028489.
GeneID910789.
GenomeReviewsGene locus ML1705 in contig AL450380_GR.
KEGGmle:ML1705.
NMPDRfig|272631.1.peg.1046.
PATRIC18056568. VBIMycLep78757_3213.

Organism-specific databases

LepromaML1705.
CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016652.
HOGENOMHBG620317.
OMAENVRTIR.
ProtClustDBPRK07956.

Enzyme and pathway databases

BioCycMLEP272631:ML1705-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 3 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_MYCLE
AccessionPrimary (citable) accession number: O33102
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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