Reviewed, UniProtKB/Swiss-Prot O33064 (FPRB_MYCLE)

Names and origin

Protein names

Recommended name:
    Probable ferredoxin/ferredoxin--NADP reductase
      Short name=FNR
    EC=1.18.1.2

Gene names

Name:	fprB
Ordered Locus Names:	ML2134
ORF Names:	MLCB57.39

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	Binds 1 or 2 4Fe-4S clusters. FAD.
Sequence similarities	In the C-terminal section; belongs to the ferredoxin--NADP reductase family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S FAD Flavoprotein Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Probable ferredoxin/ferredoxin--NADP reductase		PRO_0000167674
Regions
	Domain	2 – 29	28	4Fe-4S ferredoxin-type 1
	Domain	37 – 66	30	4Fe-4S ferredoxin-type 2
	Nucleotide binding	258 – 261	4	NADP By similarity
	Nucleotide binding	302 – 303	2	NADP By similarity
	Nucleotide binding	460 – 462	3	FAD By similarity
	Region	115 – 555	441	Ferredoxin--NADP reductase
Sites
	Metal binding	9	1	Iron-sulfur 1 By similarity
	Metal binding	15	1	Iron-sulfur 1 By similarity
	Metal binding	19	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	46	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	52	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	56	1	Iron-sulfur 1 By similarity
	Binding site	123	1	FAD; via amide nitrogen By similarity
	Binding site	143	1	FAD By similarity
	Binding site	151	1	FAD; via amide nitrogen By similarity
	Binding site	187	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	213	1	NADP By similarity
	Binding site	314	1	NADP By similarity
	Binding site	453	1	FAD; via amide nitrogen By similarity
	Binding site	460	1	NADP; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33064-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 43C7292E6A2DFED9	FASTA	555	59,712
	10 20 30 40 50 60 MPYIITQSCC NDGSCVFACP VNCIHPTPDE PGFATSEMLY IDPVACVDCG ACVSACPVGA 70 80 90 100 110 120 IASDTRLAPK QLPFIEINAS YYPARPIDLK LPPTSKLAPV IPAAQVHVRR RPLTVAIVGS 130 140 150 160 170 180 GPAAMYAADE LLTQPGVWVN VFEKLPTPYG LVRAGLAPDH QNTKKVTELF DRVAEHRRFR 190 200 210 220 230 240 FFLNVEIGRH LSHDELLAHH HAVLYAVGAP DDRRLNIDGM GIPGTGTATE LVAWINAHPD 250 260 270 280 290 300 FAYLPVDLSH ERVVVIGNGN VALDVARLLT ADPDNLARTD ISEFALHVLG GSAVREVVVA 310 320 330 340 350 360 ARRGPAHSAF TLPELIGLKA TSEVVLDAGD RKLVEGDFAT VSDSLTRKKL EVLSSLVDSS 370 380 390 400 410 420 KPTSRRRIRL AYQLTPKRVL GNQRATGVEF SVTGTEESRR FDAGLVLTSV GYRGKRIRDL 430 440 450 460 470 480 PFDEEAAVIP NDGGRVVDPS RGRPMPGAYV AGWIKRGPTG FIGTNKLCSV QTVQAVVADF 490 500 510 520 530 540 NAGWLTDPVA EPAELAKLVH ARQPDTVDSV GWRAIDAAEI AQGSTEGRPR RKFTDVADML 550 AVAAGAPPLR LRALS

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z99494 Genomic DNA. Translation: CAB16679.1. AL583924 Genomic DNA. Translation: CAC31089.1.
PIR	T45351.
RefSeq	NP_302407.1.
3D structure databases
HSSP	HSSP built from PDB template 1XER based on UniProtKB P55907.
ModBase	Search...
Genome annotation databases
GeneID	909093.
GenomeReviews	Gene locus ML2134 in contig AL450380_GR.
KEGG	mle:ML2134.
NMPDR	fig|272631.1.peg.1279.
Organism-specific databases
Leproma	ML2134.
CMR	Search...
Phylogenomic databases
HOGENOM	O33064.
OMA	O33064. AWINGHP.
Enzyme and pathway databases
BioCyc	MLEP272631:ML2134-MON.
BRENDA	1.18.1.2. 808.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR000759. Adrndx_reductase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00037. Fer4. 2 hits. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FPRB_MYCLE

Accession

Primary (citable) accession number: O33064

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)