Reviewed, UniProtKB/Swiss-Prot O33021 (LEP_MYCLE)

Names and origin

Protein names

Recommended name:
    Probable signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	ML1612
ORF Names:	MLCB250.39

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Probable signal peptidase I		PRO_0000109510
Regions
	Topological domain	1 – 53	53	Cytoplasmic Potential
	Transmembrane	54 – 74	21	Potential
	Topological domain	75 – 289	215	Extracellular Potential
Sites
	Active site	84	1	By similarity
	Active site	162	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33021-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 69F59B03944768DD	FASTA	289	31,086
	10 20 30 40 50 60 MTETTDSVPE PPSDADQLQP KVSICGLDMP AEVSETAAEA AIGVSEPKKR SALWEFAILA 70 80 90 100 110 120 VIAIGLYYVM LTFVARPYLI PSESMEPTLH GCSGCVGDRI MVDKITYRFS SPQPGDVIVF 130 140 150 160 170 180 KGPPSWNTMY KSIRSNNIVL RSVQNALSFV GFVPPDENDL VKRVIAVGGQ TVQCRSDTGL 190 200 210 220 230 240 TVNGKPLKEP YLRPVTMNAD LSFSPCLGSE FGPVTVPQGR LWVMGDNRIH SADSRYHCNS 250 260 270 280 TDVVNGLSCT GDPNSGTVPV SNVIGKARVV VWPPSRWGGV GSVNSQQGQ

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z97369 Genomic DNA. Translation: CAB10633.1. AL583922 Genomic DNA. Translation: CAC30563.1.
PIR	F87110.
RefSeq	NP_302111.1.
3D structure databases
SMR	O33021. Positions 72-284.
ModBase	Search...
Genome annotation databases
GeneID	909308.
KEGG	mle:ML1612.
NMPDR	fig|272631.1.peg.983.
Organism-specific databases
Leproma	ML1612.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG596607.
OMA	QTVECCD.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1612-MONOMER.
BRENDA	3.4.21.89. 808.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. False negative. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_MYCLE

Accession

Primary (citable) accession number: O33021

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)