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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881Manganese 2By similarity
Metal bindingi293 – 2931Manganese 1By similarity
Metal bindingi293 – 2931Manganese 2By similarity
Active sitei300 – 3001Sequence analysis
Metal bindingi311 – 3111Manganese 2By similarity
Metal bindingi370 – 3701Manganese 1By similarity
Metal bindingi372 – 3721Manganese 1By similarity
Metal bindingi372 – 3721Manganese 2By similarity
Active sitei374 – 3741Sequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Ordered Locus Names:ML0864
ORF Names:MLCB22.14
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0864.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Probable cytosol aminopeptidasePRO_0000165770Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0864.

Structurei

3D structure databases

ProteinModelPortaliO32956.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
KOiK01255.
OMAiEINRSWG.
OrthoDBiPOG091H01BT.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVTTDPGYQ GPSVHVAASL PKHDVSSSVL IVPVVSASDS DESEDRPGAV
60 70 80 90 100
VAAAEPFLTA AAVAEIEAGL RALEATGGSD QVHRLVAPSL PVGSVLTVSL
110 120 130 140 150
GKPRSEWPAD TIRRAAGVAA RSLGRAEVVI TTLAELPGES GASICSAVVE
160 170 180 190 200
GLMLGSYRFT DFRSRKTAPK DNGLRKITVL ANAKDAKKQS AHGATVASAV
210 220 230 240 250
ATARDLVNTP PSHLFPAEFA KCAKTLGESA GLEVEVLDDK ALQKAGYGGV
260 270 280 290 300
IGVGQGSSRT PRLVRLIHQG SRLAKNPKNA RKVALVGKGI TFDTGGISIK
310 320 330 340 350
PAASMHHMTS DMAGAAAVIA TITLAAQLKL PIDVVATVPM AENMPSGTAQ
360 370 380 390 400
RPGDVLTQYG GITVEVLNTD AEGRLILADA IVRACQDNPD YLIETSTLTG
410 420 430 440 450
AQTVALGSRI PGVMGSDKFR DRVAKTSQQV GENGWPMPLP DELKDDLKST
460 470 480 490 500
VADLANVSGQ RFAGMLVAGA FLREFVADGV DWAHIDIAGP AYNTDSPWGY
510 520
TPKGATGVPT RTMFAMLEDI ANHG
Length:524
Mass (Da):54,342
Last modified:January 1, 1998 - v1
Checksum:iA616AB40D6E4412F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98741 Genomic DNA. Translation: CAB11379.1.
AL583920 Genomic DNA. Translation: CAC31245.1.
PIRiT44889.
RefSeqiNP_301652.1. NC_002677.1.
WP_010907976.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31245; CAC31245; CAC31245.
GeneIDi909860.
KEGGimle:ML0864.
PATRICi18053292. VBIMycLep78757_1591.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98741 Genomic DNA. Translation: CAB11379.1.
AL583920 Genomic DNA. Translation: CAC31245.1.
PIRiT44889.
RefSeqiNP_301652.1. NC_002677.1.
WP_010907976.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO32956.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0864.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31245; CAC31245; CAC31245.
GeneIDi909860.
KEGGimle:ML0864.
PATRICi18053292. VBIMycLep78757_1591.

Organism-specific databases

LepromaiML0864.

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
KOiK01255.
OMAiEINRSWG.
OrthoDBiPOG091H01BT.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_MYCLE
AccessioniPrimary (citable) accession number: O32956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.