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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi288Manganese 2By similarity1
Metal bindingi293Manganese 1By similarity1
Metal bindingi293Manganese 2By similarity1
Active sitei300Sequence analysis1
Metal bindingi311Manganese 2By similarity1
Metal bindingi370Manganese 1By similarity1
Metal bindingi372Manganese 1By similarity1
Metal bindingi372Manganese 2By similarity1
Active sitei374Sequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-941-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Ordered Locus Names:ML0864
ORF Names:MLCB22.14
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0864

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001657701 – 524Probable cytosol aminopeptidaseAdd BLAST524

Proteomic databases

PRIDEiO32956

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0864

Structurei

3D structure databases

ProteinModelPortaliO32956
SMRiO32956
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ6 Bacteria
COG0260 LUCA
HOGENOMiHOG000243129
KOiK01255
OMAiMKNTGPR
OrthoDBiPOG091H01BT

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit

Sequencei

Sequence statusi: Complete.

O32956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVTTDPGYQ GPSVHVAASL PKHDVSSSVL IVPVVSASDS DESEDRPGAV
60 70 80 90 100
VAAAEPFLTA AAVAEIEAGL RALEATGGSD QVHRLVAPSL PVGSVLTVSL
110 120 130 140 150
GKPRSEWPAD TIRRAAGVAA RSLGRAEVVI TTLAELPGES GASICSAVVE
160 170 180 190 200
GLMLGSYRFT DFRSRKTAPK DNGLRKITVL ANAKDAKKQS AHGATVASAV
210 220 230 240 250
ATARDLVNTP PSHLFPAEFA KCAKTLGESA GLEVEVLDDK ALQKAGYGGV
260 270 280 290 300
IGVGQGSSRT PRLVRLIHQG SRLAKNPKNA RKVALVGKGI TFDTGGISIK
310 320 330 340 350
PAASMHHMTS DMAGAAAVIA TITLAAQLKL PIDVVATVPM AENMPSGTAQ
360 370 380 390 400
RPGDVLTQYG GITVEVLNTD AEGRLILADA IVRACQDNPD YLIETSTLTG
410 420 430 440 450
AQTVALGSRI PGVMGSDKFR DRVAKTSQQV GENGWPMPLP DELKDDLKST
460 470 480 490 500
VADLANVSGQ RFAGMLVAGA FLREFVADGV DWAHIDIAGP AYNTDSPWGY
510 520
TPKGATGVPT RTMFAMLEDI ANHG
Length:524
Mass (Da):54,342
Last modified:January 1, 1998 - v1
Checksum:iA616AB40D6E4412F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98741 Genomic DNA Translation: CAB11379.1
AL583920 Genomic DNA Translation: CAC31245.1
PIRiT44889
RefSeqiNP_301652.1, NC_002677.1
WP_010907976.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC31245; CAC31245; CAC31245
GeneIDi909860
KEGGimle:ML0864
PATRICifig|272631.5.peg.1591

Similar proteinsi

Entry informationi

Entry nameiAMPA_MYCLE
AccessioniPrimary (citable) accession number: O32956
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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