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O32913

- MASZ_MYCLE

UniProt

O32913 - MASZ_MYCLE

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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi439 – 4391MagnesiumUniRule annotation
Binding sitei439 – 4391GlyoxylateUniRule annotation
Metal bindingi467 – 4671MagnesiumUniRule annotation
Binding sitei548 – 5481Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei638 – 6381Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:ML2069
ORF Names:MLCB1788.27
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML2069.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731Malate synthase GPRO_0000166888Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei624 – 6241Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML2069.

Structurei

3D structure databases

ProteinModelPortaliO32913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni464 – 4674Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

O32913-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDRVSAGNL RVARVLYDFV NNEALPGTDI NPNSFWSGVA KVVADLTPQN
60 70 80 90 100
QSLLNSRDEL QAQIDKWHRH RVIEPFDVDA YRQFLIDIGY LLPEPDDFTI
110 120 130 140 150
STSGVDDEIT MTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDTIPE
160 170 180 190 200
TEGAEKGSEY NKIRGDKVIA YARKFMDQAV PLASDSWTNA TGVSIFDGQL
210 220 230 240 250
QIAIGTNSTG LASPEKFVGY NRQLRSSNWS VLLANHGLHI EVLIDPESPI
260 270 280 290 300
GKTDPVGIKD VILESAITTI MDFEDSVTAV DADDKVRGYR NWLGLNKGDL
310 320 330 340 350
TEEVNKDGKT FTRVLNADRS YTTPDGGELT LPGRSLLFVR NVGHLTTSDA
360 370 380 390 400
ILVDGGDGQE KEVFEGIIDA VFTGLAAIHG LKTGEANGPL TNSRTGSIYI
410 420 430 440 450
VKPKMHGPAE VAFTCELFSR VEDVLGLPQG TLKVGIMDEE RRTTLNLKAC
460 470 480 490 500
IKAAADRVVF INTGFLDRTG DEIHTSMEAG PMIRKGAMKN STWIKAYEDA
510 520 530 540 550
NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA GATTAWVPSP
560 570 580 590 600
TAATLHAMHY HQVDVAAVQQ ELTGQRRATV DQLLTIPLAK KLAWAPEEIR
610 620 630 640 650
EEVDNDCQSI LGYVVRWVDQ GIGCSKVPDI HNVALMEDRA TLRISSQLLA
660 670 680 690 700
NWLRHGVITS EDVRASLERM APLVDQQNAE DPAYRPMAPN FDDSIAFLAA
710 720 730
QELILSGAQQ PNGYTEPILH RRRREFKAQN R
Length:731
Mass (Da):80,142
Last modified:January 1, 1998 - v1
Checksum:i3878CADA45DB416C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL008609 Genomic DNA. Translation: CAA15459.1.
AL583924 Genomic DNA. Translation: CAC31024.1.
PIRiT44752.
RefSeqiNP_302379.1. NC_002677.1.
WP_010908699.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31024; CAC31024; CAC31024.
GeneIDi908958.
KEGGimle:ML2069.
PATRICi18057945. VBIMycLep78757_3896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL008609 Genomic DNA. Translation: CAA15459.1 .
AL583924 Genomic DNA. Translation: CAC31024.1 .
PIRi T44752.
RefSeqi NP_302379.1. NC_002677.1.
WP_010908699.1. NC_002677.1.

3D structure databases

ProteinModelPortali O32913.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML2069.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC31024 ; CAC31024 ; CAC31024 .
GeneIDi 908958.
KEGGi mle:ML2069.
PATRICi 18057945. VBIMycLep78757_3896.

Organism-specific databases

Lepromai ML2069.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiMASZ_MYCLE
AccessioniPrimary (citable) accession number: O32913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3