Reviewed, UniProtKB/Swiss-Prot O32913 (MASZ_MYCLE)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	ML2069
ORF Names:	MLCB1788.27

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	Malate synthase G HAMAP MF_00641		PRO_0000166888
Sites
	Active site	340	1	Proton acceptor By similarity
	Active site	638	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O32913-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3878CADA45DB416C	FASTA	731	80,142
	10 20 30 40 50 60 MTDRVSAGNL RVARVLYDFV NNEALPGTDI NPNSFWSGVA KVVADLTPQN QSLLNSRDEL 70 80 90 100 110 120 QAQIDKWHRH RVIEPFDVDA YRQFLIDIGY LLPEPDDFTI STSGVDDEIT MTAGPQLVVP 130 140 150 160 170 180 VLNARFALNA ANARWGSLYD ALYGTDTIPE TEGAEKGSEY NKIRGDKVIA YARKFMDQAV 190 200 210 220 230 240 PLASDSWTNA TGVSIFDGQL QIAIGTNSTG LASPEKFVGY NRQLRSSNWS VLLANHGLHI 250 260 270 280 290 300 EVLIDPESPI GKTDPVGIKD VILESAITTI MDFEDSVTAV DADDKVRGYR NWLGLNKGDL 310 320 330 340 350 360 TEEVNKDGKT FTRVLNADRS YTTPDGGELT LPGRSLLFVR NVGHLTTSDA ILVDGGDGQE 370 380 390 400 410 420 KEVFEGIIDA VFTGLAAIHG LKTGEANGPL TNSRTGSIYI VKPKMHGPAE VAFTCELFSR 430 440 450 460 470 480 VEDVLGLPQG TLKVGIMDEE RRTTLNLKAC IKAAADRVVF INTGFLDRTG DEIHTSMEAG 490 500 510 520 530 540 PMIRKGAMKN STWIKAYEDA NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA 550 560 570 580 590 600 GATTAWVPSP TAATLHAMHY HQVDVAAVQQ ELTGQRRATV DQLLTIPLAK KLAWAPEEIR 610 620 630 640 650 660 EEVDNDCQSI LGYVVRWVDQ GIGCSKVPDI HNVALMEDRA TLRISSQLLA NWLRHGVITS 670 680 690 700 710 720 EDVRASLERM APLVDQQNAE DPAYRPMAPN FDDSIAFLAA QELILSGAQQ PNGYTEPILH 730 RRRREFKAQN R

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL008609 Genomic DNA. Translation: CAA15459.1. AL583924 Genomic DNA. Translation: CAC31024.1.
PIR	T44752.
RefSeq	NP_302379.1.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB Q50596.
ModBase	Search...
Genome annotation databases
GeneID	908958.
GenomeReviews	Gene locus ML2069 in contig AL450380_GR.
KEGG	mle:ML2069.
NMPDR	fig|272631.1.peg.1251.
Organism-specific databases
Leproma	ML2069.
CMR	Search...
Phylogenomic databases
HOGENOM	O32913.
OMA	PKMHGPD.
Enzyme and pathway databases
BioCyc	MLEP272631:ML2069-MON.
BRENDA	2.3.3.9. 808.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_MYCLE

Accession

Primary (citable) accession number: O32913

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)