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Protein

Bifunctional protein FolD

Gene

folD

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192NADPUniRule annotation1
Binding sitei233NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 167NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:ML0674
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0674.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002684011 – 282Bifunctional protein FolDAdd BLAST282

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0674.

Structurei

3D structure databases

ProteinModelPortaliO32879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.
OrthoDBiPOG091H0041.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

O32879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAITLDGKA TRDEILIDLK QRVAALTESG RTPGLGTILV GDDPGSHAYV
60 70 80 90 100
RGKHADCAKV GITSIRRDLP VDITTAVLHD TIEELNANPD CTGYIVQLPL
110 120 130 140 150
PKYLDENTAL ERVDPAKDAD GLHPTNLGRL VLSTPAPLPC TARGILHLLR
160 170 180 190 200
RYGVEIAGTH VVIIGRGVTV GRPLGLLLTR RSENATVTLC HTGTRNLAAL
210 220 230 240 250
TKQADIIVAA VGVPHLLTAD MVRPGAVVVD VGVSRVETRL VGDVHPDVWE
260 270 280
VAGHVSPNPG GVGPLTRVFL LTNVVELAEG RQ
Length:282
Mass (Da):29,835
Last modified:January 1, 1998 - v1
Checksum:iB268447C220162F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98271 Genomic DNA. Translation: CAB10998.1.
AL583919 Genomic DNA. Translation: CAC30183.1.
PIRiT45307.
RefSeqiNP_301545.1. NC_002677.1.
WP_010907869.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30183; CAC30183; CAC30183.
GeneIDi909678.
KEGGimle:ML0674.
PATRICi18052508. VBIMycLep78757_1200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98271 Genomic DNA. Translation: CAB10998.1.
AL583919 Genomic DNA. Translation: CAC30183.1.
PIRiT45307.
RefSeqiNP_301545.1. NC_002677.1.
WP_010907869.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO32879.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30183; CAC30183; CAC30183.
GeneIDi909678.
KEGGimle:ML0674.
PATRICi18052508. VBIMycLep78757_1200.

Organism-specific databases

LepromaiML0674.

Phylogenomic databases

eggNOGiCOG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.
OrthoDBiPOG091H0041.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD_MYCLE
AccessioniPrimary (citable) accession number: O32879
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.