O32823 (TRXB_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thioredoxin reductase
Short name=TRXR
EC=1.8.1.9

Gene names

Name:	trxB
Ordered Locus Names:	lmo2478

Organism

Listeria monocytogenes

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Thioredoxin reductase		PRO_0000166736
Regions
	Nucleotide binding	37 – 44	8	FAD By similarity
	Nucleotide binding	279 – 288	10	FAD By similarity
Amino acid modifications
	Disulfide bond	136 ↔ 139		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	O32823 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8D42F36970611979	FASTA	319	34,174
	10 20 30 40 50 60 MASEEKIYDV IIIGAGPAGM TAALYTSRAD LDTLMIERGV PGGQMVNTAE VENYPGFDSI 70 80 90 100 110 120 LGPDLSDKML SGAKQFGAEY AYGDIKEVVD GKEFKTVTAG SKTYKARAII IATGAEHRKL 130 140 150 160 170 180 GAAGEEELSG RGVSYCAVCD GAFFKNRELI VVGGGDSAVE EGTYLTRYAD KVTIVHRRDK 190 200 210 220 230 240 LRAQQILQDR AFKDEKVDFI WNSTVEEIVG DGKKVTGAKL VSTVDGSESI MPVDGVFIYV 250 260 270 280 290 300 GLVPLTKAFL NLGITDDEGY IVTDEEMRTN LPGIFAAGDV RAKSLRQIVT ATGDGGLAGQ 310 NAQKYVEELK ESLEAEAAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, cloning and characterization of the Listeria monocytogenes thioredoxin reductase gene, trxB." Borovok I., Mislovati M., Cohen G., Aharonowitz Y. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EGD / Serovar 1/2a.
[2]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF009622 Genomic DNA. Translation: AAB63804.1. AL591983 Genomic DNA. Translation: CAD00556.1.
PIR	AF1384.
RefSeq	NP_466001.1. NC_003210.1.
3D structure databases
ProteinModelPortal	O32823.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	987338.
GenomeReviews	Gene locus lmo2478 in contig AL591824_GR.
KEGG	lmo:lmo2478.
PATRIC	20314233. VBILisMon69206_2538.
Organism-specific databases
GenoList	LMO2478.
Phylogenomic databases
HOGENOM	HBG669726.
OMA	GEEREFP.
ProtClustDB	CLSK2753262.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2478-MONOMER.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
KO	K00384.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_LISMO

Accession

Primary (citable) accession number: O32823

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program