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Protein

DNA polymerase I

Gene

polA

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
Ordered Locus Names:llmg_2425
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000000364 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012441 – 877DNA polymerase IAdd BLAST877

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.

Protein-protein interaction databases

STRINGi416870.llmg_2425.

Structurei

3D structure databases

ProteinModelPortaliO32801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated
Contains 1 3'-5' exonuclease domain.Curated
Contains 1 5'-3' exonuclease domain.Curated

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.
HOGENOMiHOG000020998.
KOiK02335.
OMAiCFDTETT.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDKNRLLLI DGSSVAFRAF FALYNQLDRF KAPNGLHTNA IFAFHTMLSS
60 70 80 90 100
LMERIQPTHV LIAFDAGKTT FRTEMFADYK GGRSKTPDEF REQLPFIKEM
110 120 130 140 150
IEKLGIRHYE LANYEADDII GTLDKMAEAP DVNFDVTIVT GDKDMIQLVD
160 170 180 190 200
GNTRVEISKK GVAEFEEFTP DYLLEKMGLT PSQFIDLKAL MGDSSDNYPG
210 220 230 240 250
VTKVGEKTGL KLLQEFGSLE NLYENVETLK ASKMKDNLIA DKEMAFLSQQ
260 270 280 290 300
LATINTKAPL EIGLEDTLLK EKNVAELGQF YDEMGFSQFK SKLLADGGGE
310 320 330 340 350
VTDETVSEEI KFEIVTDKSS VASVNADDFF YLETLGENYH REQIVAFAWG
360 370 380 390 400
NSEKIYVSKN LDLLTEMKFP ENTYDFKKNR VLLSHLNIEL PLVKFDAMLA
410 420 430 440 450
KYLISTTEDN KISTIARLFD VGHLATDEEI FGKGTKLALP DDEILFDHLA
460 470 480 490 500
RKIRVLARAK EKMMAELIEN EQEHLLSDME LPLAEVLAKM EITGISVSQN
510 520 530 540 550
TLEEIGAENE EKLASLTREI YDLAGEEFNI NSPKQLGVIL FEKLQLPVGK
560 570 580 590 600
KTKTGYSTAV DVLEDLAALS PVVAKILEYR QINKVQSTYV KGLIPQIADD
610 620 630 640 650
GKIHTRYVQD LTQTGRLSSV DPNLQNIPVR LEEGRKIRKA FVPSQDSLLL
660 670 680 690 700
SSDYSQIELR VLAHISADEH LIDAFKHGAD IHTSTAMRVF GIEKAEDVTA
710 720 730 740 750
NDRRNAKAVN FGVVYGISDF GLARNLGITR KDAKNYIETY FERYPGIKTY
760 770 780 790 800
MENIVREARD KGFVETMSHR RRKIPDINAR NFNVRGFAER TAINSPIQGS
810 820 830 840 850
AADILKIAMI NLDKALTERQ SKSKLLLQVH DEIILDVPLE ELEDIKALVK
860 870
QTMEEAIELA VPLKVDDNTG KTWYEAK
Length:877
Mass (Da):98,866
Last modified:May 1, 2007 - v2
Checksum:i214179C9CDC3A983
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti585V → E in AAB64184 (PubMed:9226255).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78771 Genomic DNA. Translation: AAB64184.1.
AM406671 Genomic DNA. Translation: CAL98989.1.
RefSeqiWP_011836063.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL98989; CAL98989; llmg_2425.
KEGGillm:llmg_2425.
PATRICi22286066. VBILacLac4574_2471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78771 Genomic DNA. Translation: AAB64184.1.
AM406671 Genomic DNA. Translation: CAL98989.1.
RefSeqiWP_011836063.1. NC_009004.1.

3D structure databases

ProteinModelPortaliO32801.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416870.llmg_2425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL98989; CAL98989; llmg_2425.
KEGGillm:llmg_2425.
PATRICi22286066. VBILacLac4574_2471.

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.
HOGENOMiHOG000020998.
KOiK02335.
OMAiCFDTETT.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO1_LACLM
AccessioniPrimary (citable) accession number: O32801
Secondary accession number(s): A2RNU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.