polA

Functionⁱ

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.By similarity

Catalytic activityⁱ

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functionⁱ

3'-5' exonuclease activity Source: InterPro
DNA binding Source: UniProtKB-KW
DNA-directed DNA polymerase activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA-dependent DNA replication Source: InterPro
DNA repair Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological process	DNA damage, DNA repair, DNA replication

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA polymerase I (EC:2.7.7.7) Short name: POL I
Gene namesⁱ	Name:polA Ordered Locus Names:llmg_2425
Organismⁱ	Lactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifierⁱ	416870 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. cremoris
Proteomesⁱ	UP000000364 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000101244	1 – 877	DNA polymerase IAdd BLAST		877

Proteomic databases

PRIDEⁱ	O32801

PRIDEⁱ

O32801

Interactionⁱ

Subunit structureⁱ

Single-chain monomer with multiple functions.

Protein-protein interaction databases

STRINGⁱ	416870.llmg_2425

STRINGⁱ

416870.llmg_2425

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O32801
SMRⁱ	O32801
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	180 – 272	5'-3' exonucleaseSequence analysisAdd BLAST		93
Domainⁱ	312 – 468	3'-5' exonucleaseSequence analysisAdd BLAST		157

Sequence similaritiesⁱ

Belongs to the DNA polymerase type-A family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105C2M Bacteria COG0258 LUCA COG0749 LUCA
HOGENOMⁱ	HOG000020998
KEGG Orthology (KO) More...KOⁱ	K02335
OMAⁱ	ETGRVHT

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd09898 H3TH_53EXO, 1 hit
Gene3Dⁱ	3.30.420.10, 1 hit
InterProⁱ	View protein in InterPro IPR002562 3'-5'_exonuclease_dom IPR020046 5-3_exonucl_a-hlix_arch_N IPR036279 5-3_exonuclease_C_sf IPR002421 5-3_exonuclease_N IPR019760 DNA-dir_DNA_pol_A_CS IPR001098 DNA-dir_DNA_pol_A_palm_dom IPR020045 DNA_polI_H3TH IPR018320 DNA_polymerase_1 IPR002298 DNA_polymerase_A IPR008918 HhH2 IPR029060 PIN-like_dom_sf IPR012337 RNaseH-like_sf IPR036397 RNaseH_sf
PANTHERⁱ	PTHR10133 PTHR10133, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01367 5_3_exonuc, 1 hit PF02739 5_3_exonuc_N, 1 hit PF00476 DNA_pol_A, 1 hit
PRINTSⁱ	PR00868 DNAPOLI
SMARTⁱ	View protein in SMART SM00474 35EXOc, 1 hit SM00475 53EXOc, 1 hit SM00279 HhH2, 1 hit SM00482 POLAc, 1 hit
SUPFAMⁱ	SSF47807 SSF47807, 1 hit SSF53098 SSF53098, 1 hit SSF88723 SSF88723, 1 hit
TIGRFAMsⁱ	TIGR00593 pola, 1 hit
PROSITEⁱ	View protein in PROSITE PS00447 DNA_POLYMERASE_A, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O32801-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEDKNRLLLI DGSSVAFRAF FALYNQLDRF KAPNGLHTNA IFAFHTMLSS 
        60         70         80         90        100
LMERIQPTHV LIAFDAGKTT FRTEMFADYK GGRSKTPDEF REQLPFIKEM 
       110        120        130        140        150
IEKLGIRHYE LANYEADDII GTLDKMAEAP DVNFDVTIVT GDKDMIQLVD 
       160        170        180        190        200
GNTRVEISKK GVAEFEEFTP DYLLEKMGLT PSQFIDLKAL MGDSSDNYPG 
       210        220        230        240        250
VTKVGEKTGL KLLQEFGSLE NLYENVETLK ASKMKDNLIA DKEMAFLSQQ 
       260        270        280        290        300
LATINTKAPL EIGLEDTLLK EKNVAELGQF YDEMGFSQFK SKLLADGGGE 
       310        320        330        340        350
VTDETVSEEI KFEIVTDKSS VASVNADDFF YLETLGENYH REQIVAFAWG 
       360        370        380        390        400
NSEKIYVSKN LDLLTEMKFP ENTYDFKKNR VLLSHLNIEL PLVKFDAMLA 
       410        420        430        440        450
KYLISTTEDN KISTIARLFD VGHLATDEEI FGKGTKLALP DDEILFDHLA 
       460        470        480        490        500
RKIRVLARAK EKMMAELIEN EQEHLLSDME LPLAEVLAKM EITGISVSQN 
       510        520        530        540        550
TLEEIGAENE EKLASLTREI YDLAGEEFNI NSPKQLGVIL FEKLQLPVGK 
       560        570        580        590        600
KTKTGYSTAV DVLEDLAALS PVVAKILEYR QINKVQSTYV KGLIPQIADD 
       610        620        630        640        650
GKIHTRYVQD LTQTGRLSSV DPNLQNIPVR LEEGRKIRKA FVPSQDSLLL 
       660        670        680        690        700
SSDYSQIELR VLAHISADEH LIDAFKHGAD IHTSTAMRVF GIEKAEDVTA 
       710        720        730        740        750
NDRRNAKAVN FGVVYGISDF GLARNLGITR KDAKNYIETY FERYPGIKTY 
       760        770        780        790        800
MENIVREARD KGFVETMSHR RRKIPDINAR NFNVRGFAER TAINSPIQGS 
       810        820        830        840        850
AADILKIAMI NLDKALTERQ SKSKLLLQVH DEIILDVPLE ELEDIKALVK 
       860        870 
QTMEEAIELA VPLKVDDNTG KTWYEAK

Length:877

Mass (Da):98,866

Last modified:May 1, 2007 - v2

Checksum:ⁱ214179C9CDC3A983

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	585	V → E in AAB64184 (PubMed:9226255).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U78771 Genomic DNA Translation: AAB64184.1 AM406671 Genomic DNA Translation: CAL98989.1
NCBI Reference Sequences More...RefSeqⁱ	WP_011836063.1, NC_009004.1

Genome annotation databases

EnsemblBacteriaⁱ	CAL98989; CAL98989; llmg_2425
KEGGⁱ	llm:llmg_2425

Protein	Similar proteins		Species	Score	Length
O32801	DNA polymerase I		LACLA		877
DNA polymerase I		Lactococcus lactis		877
DNA polymerase I		Lactococcus lactis subsp. lactis KLDS 4.0325		877
DNA polymerase I		LACLL		877
DNA polymerase I		Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2		877
+63

Protein	Similar proteins		Species	Score	Length
O32801	DNA polymerase I		STRR6		877
DNA polymerase I		STRP2		877
DNA polymerase I		STROR		910
DNA polymerase I		Streptococcus mitis SK616		895
UPI00046C4D22		STRMT		877
+1473

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U78771 Genomic DNA Translation: AAB64184.1 AM406671 Genomic DNA Translation: CAL98989.1
NCBI Reference Sequences More...RefSeqⁱ	WP_011836063.1, NC_009004.1

3D structure databases

ProteinModelPortalⁱ	O32801
SMRⁱ	O32801
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	416870.llmg_2425

STRINGⁱ

416870.llmg_2425

Proteomic databases

PRIDEⁱ	O32801

PRIDEⁱ

O32801

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	CAL98989; CAL98989; llmg_2425
KEGGⁱ	llm:llmg_2425

Phylogenomic databases

eggNOGⁱ	ENOG4105C2M Bacteria COG0258 LUCA COG0749 LUCA
HOGENOMⁱ	HOG000020998
KEGG Orthology (KO) More...KOⁱ	K02335
OMAⁱ	ETGRVHT

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd09898 H3TH_53EXO, 1 hit
Gene3Dⁱ	3.30.420.10, 1 hit
InterProⁱ	View protein in InterPro IPR002562 3'-5'_exonuclease_dom IPR020046 5-3_exonucl_a-hlix_arch_N IPR036279 5-3_exonuclease_C_sf IPR002421 5-3_exonuclease_N IPR019760 DNA-dir_DNA_pol_A_CS IPR001098 DNA-dir_DNA_pol_A_palm_dom IPR020045 DNA_polI_H3TH IPR018320 DNA_polymerase_1 IPR002298 DNA_polymerase_A IPR008918 HhH2 IPR029060 PIN-like_dom_sf IPR012337 RNaseH-like_sf IPR036397 RNaseH_sf
PANTHERⁱ	PTHR10133 PTHR10133, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01367 5_3_exonuc, 1 hit PF02739 5_3_exonuc_N, 1 hit PF00476 DNA_pol_A, 1 hit
PRINTSⁱ	PR00868 DNAPOLI
SMARTⁱ	View protein in SMART SM00474 35EXOc, 1 hit SM00475 53EXOc, 1 hit SM00279 HhH2, 1 hit SM00482 POLAc, 1 hit
SUPFAMⁱ	SSF47807 SSF47807, 1 hit SSF53098 SSF53098, 1 hit SSF88723 SSF88723, 1 hit
TIGRFAMsⁱ	TIGR00593 pola, 1 hit
PROSITEⁱ	View protein in PROSITE PS00447 DNA_POLYMERASE_A, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DPO1_LACLM
Accessionⁱ	O32801Primary (citable) accession number: O32801 Secondary accession number(s): A2RNU5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
	Last sequence update:	May 1, 2007
	Last modified:	May 23, 2018
	This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

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UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - O32801 (DPO1_LACLM)

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DNA polymerase I

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

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Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

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3D structure databases

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Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

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3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ