polA

Functionⁱ

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.By similarity

Catalytic activityⁱ

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functionⁱ

3'-5' exonuclease activity Source: InterPro
DNA binding Source: UniProtKB-KW
DNA-directed DNA polymerase activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA-dependent DNA replication Source: InterPro
DNA repair Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological process	DNA damage, DNA repair, DNA replication

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA polymerase I (EC:2.7.7.7) Short name: POL I
Gene namesⁱ	Name:polA Ordered Locus Names:llmg_2425
Organismⁱ	Lactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifierⁱ	416870 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. cremoris
Proteomesⁱ	UP000000364 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000101244	1 – 877	DNA polymerase IAdd BLAST		877

Interactionⁱ

Subunit structureⁱ

Single-chain monomer with multiple functions.

Protein-protein interaction databases

STRINGⁱ	416870.llmg_2425.

STRINGⁱ

416870.llmg_2425.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O32801.
SMRⁱ	O32801.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	180 – 272	5'-3' exonucleaseSequence analysisAdd BLAST		93
Domainⁱ	312 – 468	3'-5' exonucleaseSequence analysisAdd BLAST		157

Sequence similaritiesⁱ

Belongs to the DNA polymerase type-A family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105C2M. Bacteria. COG0258. LUCA. COG0749. LUCA.
HOGENOMⁱ	HOG000020998.
KEGG Orthology (KO) More...KOⁱ	K02335.
OMAⁱ	ETGRVHT.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd09898. H3TH_53EXO. 1 hit.
Gene3Dⁱ	3.30.420.10. 1 hit.
InterProⁱ	View protein in InterPro IPR002562. 3'-5'_exonuclease_dom. IPR020046. 5-3_exonucl_a-hlix_arch_N. IPR036279. 5-3_exonuclease_C_sf. IPR002421. 5-3_exonuclease_N. IPR019760. DNA-dir_DNA_pol_A_CS. IPR001098. DNA-dir_DNA_pol_A_palm_dom. IPR020045. DNA_polI_H3TH. IPR018320. DNA_polymerase_1. IPR002298. DNA_polymerase_A. IPR008918. HhH2. IPR029060. PIN-like_dom_sf. IPR012337. RNaseH-like_sf. IPR036397. RNaseH_sf.
PANTHERⁱ	PTHR10133. PTHR10133. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01367. 5_3_exonuc. 1 hit. PF02739. 5_3_exonuc_N. 1 hit. PF00476. DNA_pol_A. 1 hit.
PRINTSⁱ	PR00868. DNAPOLI.
SMARTⁱ	View protein in SMART SM00474. 35EXOc. 1 hit. SM00475. 53EXOc. 1 hit. SM00279. HhH2. 1 hit. SM00482. POLAc. 1 hit.
SUPFAMⁱ	SSF47807. SSF47807. 1 hit. SSF53098. SSF53098. 1 hit. SSF88723. SSF88723. 1 hit.
TIGRFAMsⁱ	TIGR00593. pola. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00447. DNA_POLYMERASE_A. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

O32801-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEDKNRLLLI DGSSVAFRAF FALYNQLDRF KAPNGLHTNA IFAFHTMLSS 
        60         70         80         90        100
LMERIQPTHV LIAFDAGKTT FRTEMFADYK GGRSKTPDEF REQLPFIKEM 
       110        120        130        140        150
IEKLGIRHYE LANYEADDII GTLDKMAEAP DVNFDVTIVT GDKDMIQLVD 
       160        170        180        190        200
GNTRVEISKK GVAEFEEFTP DYLLEKMGLT PSQFIDLKAL MGDSSDNYPG 
       210        220        230        240        250
VTKVGEKTGL KLLQEFGSLE NLYENVETLK ASKMKDNLIA DKEMAFLSQQ 
       260        270        280        290        300
LATINTKAPL EIGLEDTLLK EKNVAELGQF YDEMGFSQFK SKLLADGGGE 
       310        320        330        340        350
VTDETVSEEI KFEIVTDKSS VASVNADDFF YLETLGENYH REQIVAFAWG 
       360        370        380        390        400
NSEKIYVSKN LDLLTEMKFP ENTYDFKKNR VLLSHLNIEL PLVKFDAMLA 
       410        420        430        440        450
KYLISTTEDN KISTIARLFD VGHLATDEEI FGKGTKLALP DDEILFDHLA 
       460        470        480        490        500
RKIRVLARAK EKMMAELIEN EQEHLLSDME LPLAEVLAKM EITGISVSQN 
       510        520        530        540        550
TLEEIGAENE EKLASLTREI YDLAGEEFNI NSPKQLGVIL FEKLQLPVGK 
       560        570        580        590        600
KTKTGYSTAV DVLEDLAALS PVVAKILEYR QINKVQSTYV KGLIPQIADD 
       610        620        630        640        650
GKIHTRYVQD LTQTGRLSSV DPNLQNIPVR LEEGRKIRKA FVPSQDSLLL 
       660        670        680        690        700
SSDYSQIELR VLAHISADEH LIDAFKHGAD IHTSTAMRVF GIEKAEDVTA 
       710        720        730        740        750
NDRRNAKAVN FGVVYGISDF GLARNLGITR KDAKNYIETY FERYPGIKTY 
       760        770        780        790        800
MENIVREARD KGFVETMSHR RRKIPDINAR NFNVRGFAER TAINSPIQGS 
       810        820        830        840        850
AADILKIAMI NLDKALTERQ SKSKLLLQVH DEIILDVPLE ELEDIKALVK 
       860        870 
QTMEEAIELA VPLKVDDNTG KTWYEAK

Length:877

Mass (Da):98,866

Last modified:May 1, 2007 - v2

Checksum:ⁱ214179C9CDC3A983

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	585	V → E in AAB64184 (PubMed:9226255).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U78771 Genomic DNA. Translation: AAB64184.1. AM406671 Genomic DNA. Translation: CAL98989.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011836063.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaⁱ	CAL98989; CAL98989; llmg_2425.
KEGGⁱ	llm:llmg_2425.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
O32801	A0A165G2E8	Lactococcus lactis subsp. cremoris (Streptococcus cremoris)	877	UniRef100_O32801	Cluster: DNA polymerase I	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
O32801	Q9CDS1 A0A269YSF5 A0A0V8EMI8 T0WG37 U5PR68 G6FBV8 A0A1V0NJ38 A0A0V8BHT0 UPI00071C2D31 A0A0V8B4B3 +57	Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) Lactococcus lactis Lactococcus lactis subsp. lactis (Streptococcus lactis) Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2 Lactococcus lactis subsp. lactis KLDS 4.0325 Lactococcus lactis subsp. lactis CNCM I-1631 Lactococcus lactis subsp. lactis Dephy 1 Lactococcus lactis subsp. lactis IO-1 Lactococcus lactis subsp. cremoris (Streptococcus cremoris) Lactococcus lactis subsp. cremoris TIFN1 And more	877	UniRef90_Q9CDS1	Cluster: DNA polymerase I	68

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
O32801	P59200 A0A0H2ZQE1 A0A139RNV5 UPI00077D80D5 A0A139NVZ1 UPI0007764DE4 A0A139NZV0 UPI0007764408 I0SJF9 UPI00046C4D22 +1333	Streptococcus pneumoniae (strain ATCC BAA-255 / R6) Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) Streptococcus oralis Streptococcus mitis SK616 Streptococcus mitis Streptococcus mitis SK575 Streptococcus sp. M334 Streptococcus pneumoniae Streptococcus sp. F0441 Streptococcus mitis 21/39 And more	877	UniRef50_P59200	Cluster: DNA polymerase I	1,344

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U78771 Genomic DNA. Translation: AAB64184.1. AM406671 Genomic DNA. Translation: CAL98989.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011836063.1. NC_009004.1.

3D structure databases

ProteinModelPortalⁱ	O32801.
SMRⁱ	O32801.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	416870.llmg_2425.

STRINGⁱ

416870.llmg_2425.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	CAL98989; CAL98989; llmg_2425.
KEGGⁱ	llm:llmg_2425.

Phylogenomic databases

eggNOGⁱ	ENOG4105C2M. Bacteria. COG0258. LUCA. COG0749. LUCA.
HOGENOMⁱ	HOG000020998.
KEGG Orthology (KO) More...KOⁱ	K02335.
OMAⁱ	ETGRVHT.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd09898. H3TH_53EXO. 1 hit.
Gene3Dⁱ	3.30.420.10. 1 hit.
InterProⁱ	View protein in InterPro IPR002562. 3'-5'_exonuclease_dom. IPR020046. 5-3_exonucl_a-hlix_arch_N. IPR036279. 5-3_exonuclease_C_sf. IPR002421. 5-3_exonuclease_N. IPR019760. DNA-dir_DNA_pol_A_CS. IPR001098. DNA-dir_DNA_pol_A_palm_dom. IPR020045. DNA_polI_H3TH. IPR018320. DNA_polymerase_1. IPR002298. DNA_polymerase_A. IPR008918. HhH2. IPR029060. PIN-like_dom_sf. IPR012337. RNaseH-like_sf. IPR036397. RNaseH_sf.
PANTHERⁱ	PTHR10133. PTHR10133. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01367. 5_3_exonuc. 1 hit. PF02739. 5_3_exonuc_N. 1 hit. PF00476. DNA_pol_A. 1 hit.
PRINTSⁱ	PR00868. DNAPOLI.
SMARTⁱ	View protein in SMART SM00474. 35EXOc. 1 hit. SM00475. 53EXOc. 1 hit. SM00279. HhH2. 1 hit. SM00482. POLAc. 1 hit.
SUPFAMⁱ	SSF47807. SSF47807. 1 hit. SSF53098. SSF53098. 1 hit. SSF88723. SSF88723. 1 hit.
TIGRFAMsⁱ	TIGR00593. pola. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00447. DNA_POLYMERASE_A. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DPO1_LACLM
Accessionⁱ	O32801Primary (citable) accession number: O32801 Secondary accession number(s): A2RNU5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
	Last sequence update:	May 1, 2007
	Last modified:	March 28, 2018
	This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB - O32801 (DPO1_LACLM)

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DNA polymerase I

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Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Molecule processing

Interactionⁱ

Subunit structureⁱ

Protein-protein interaction databases

Structureⁱ

3D structure databases

Family & Domainsⁱ

Domains and Repeats

Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Experimental Info

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O32801 (DPO1_LACLM)

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DNA polymerase I

polA

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ