UniProtKB - O32801 (DPO1_LACLM)
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Protein
DNA polymerase I
Gene
polA
Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Functioni
In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.By similarity
Catalytic activityi
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
GO - Molecular functioni
- 3'-5' exonuclease activity Source: InterPro
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- DNA-dependent DNA replication Source: InterPro
- DNA repair Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:polA Ordered Locus Names:llmg_2425 |
Organismi | Lactococcus lactis subsp. cremoris (strain MG1363) |
Taxonomic identifieri | 416870 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101244 | 1 – 877 | DNA polymerase IAdd BLAST | 877 |
Interactioni
Subunit structurei
Single-chain monomer with multiple functions.
Protein-protein interaction databases
STRINGi | 416870.llmg_2425. |
Structurei
3D structure databases
ProteinModelPortali | O32801. |
SMRi | O32801. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 180 – 272 | 5'-3' exonucleaseSequence analysisAdd BLAST | 93 | |
Domaini | 312 – 468 | 3'-5' exonucleaseSequence analysisAdd BLAST | 157 |
Sequence similaritiesi
Belongs to the DNA polymerase type-A family.Curated
Phylogenomic databases
eggNOGi | ENOG4105C2M. Bacteria. COG0258. LUCA. COG0749. LUCA. |
HOGENOMi | HOG000020998. |
KOi | K02335. |
OMAi | ETGRVHT. |
Family and domain databases
CDDi | cd09898. H3TH_53EXO. 1 hit. |
Gene3Di | 3.30.420.10. 1 hit. |
InterProi | View protein in InterPro IPR002562. 3'-5'_exonuclease_dom. IPR020046. 5-3_exonucl_a-hlix_arch_N. IPR036279. 5-3_exonuclease_C_sf. IPR002421. 5-3_exonuclease_N. IPR019760. DNA-dir_DNA_pol_A_CS. IPR001098. DNA-dir_DNA_pol_A_palm_dom. IPR020045. DNA_polI_H3TH. IPR018320. DNA_polymerase_1. IPR002298. DNA_polymerase_A. IPR008918. HhH2. IPR029060. PIN-like_dom_sf. IPR012337. RNaseH-like_sf. IPR036397. RNaseH_sf. |
PANTHERi | PTHR10133. PTHR10133. 1 hit. |
Pfami | View protein in Pfam PF01367. 5_3_exonuc. 1 hit. PF02739. 5_3_exonuc_N. 1 hit. PF00476. DNA_pol_A. 1 hit. |
PRINTSi | PR00868. DNAPOLI. |
SMARTi | View protein in SMART SM00474. 35EXOc. 1 hit. SM00475. 53EXOc. 1 hit. SM00279. HhH2. 1 hit. SM00482. POLAc. 1 hit. |
SUPFAMi | SSF47807. SSF47807. 1 hit. SSF53098. SSF53098. 1 hit. SSF88723. SSF88723. 1 hit. |
TIGRFAMsi | TIGR00593. pola. 1 hit. |
PROSITEi | View protein in PROSITE PS00447. DNA_POLYMERASE_A. 1 hit. |
i Sequence
Sequence statusi: Complete.
O32801-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDKNRLLLI DGSSVAFRAF FALYNQLDRF KAPNGLHTNA IFAFHTMLSS
60 70 80 90 100
LMERIQPTHV LIAFDAGKTT FRTEMFADYK GGRSKTPDEF REQLPFIKEM
110 120 130 140 150
IEKLGIRHYE LANYEADDII GTLDKMAEAP DVNFDVTIVT GDKDMIQLVD
160 170 180 190 200
GNTRVEISKK GVAEFEEFTP DYLLEKMGLT PSQFIDLKAL MGDSSDNYPG
210 220 230 240 250
VTKVGEKTGL KLLQEFGSLE NLYENVETLK ASKMKDNLIA DKEMAFLSQQ
260 270 280 290 300
LATINTKAPL EIGLEDTLLK EKNVAELGQF YDEMGFSQFK SKLLADGGGE
310 320 330 340 350
VTDETVSEEI KFEIVTDKSS VASVNADDFF YLETLGENYH REQIVAFAWG
360 370 380 390 400
NSEKIYVSKN LDLLTEMKFP ENTYDFKKNR VLLSHLNIEL PLVKFDAMLA
410 420 430 440 450
KYLISTTEDN KISTIARLFD VGHLATDEEI FGKGTKLALP DDEILFDHLA
460 470 480 490 500
RKIRVLARAK EKMMAELIEN EQEHLLSDME LPLAEVLAKM EITGISVSQN
510 520 530 540 550
TLEEIGAENE EKLASLTREI YDLAGEEFNI NSPKQLGVIL FEKLQLPVGK
560 570 580 590 600
KTKTGYSTAV DVLEDLAALS PVVAKILEYR QINKVQSTYV KGLIPQIADD
610 620 630 640 650
GKIHTRYVQD LTQTGRLSSV DPNLQNIPVR LEEGRKIRKA FVPSQDSLLL
660 670 680 690 700
SSDYSQIELR VLAHISADEH LIDAFKHGAD IHTSTAMRVF GIEKAEDVTA
710 720 730 740 750
NDRRNAKAVN FGVVYGISDF GLARNLGITR KDAKNYIETY FERYPGIKTY
760 770 780 790 800
MENIVREARD KGFVETMSHR RRKIPDINAR NFNVRGFAER TAINSPIQGS
810 820 830 840 850
AADILKIAMI NLDKALTERQ SKSKLLLQVH DEIILDVPLE ELEDIKALVK
860 870
QTMEEAIELA VPLKVDDNTG KTWYEAK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 585 | V → E in AAB64184 (PubMed:9226255).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78771 Genomic DNA. Translation: AAB64184.1. AM406671 Genomic DNA. Translation: CAL98989.1. |
RefSeqi | WP_011836063.1. NC_009004.1. |
Genome annotation databases
EnsemblBacteriai | CAL98989; CAL98989; llmg_2425. |
KEGGi | llm:llmg_2425. |
Similar proteinsi
Entry informationi
Entry namei | DPO1_LACLM | |
Accessioni | O32801Primary (citable) accession number: O32801 Secondary accession number(s): A2RNU5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | May 1, 2007 | |
Last modified: | March 28, 2018 | |
This is version 117 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |