Reviewed, UniProtKB/Swiss-Prot O32765 (LDH_LACHE)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase
      Short name=L-LDH
    EC=1.1.1.27

Gene names

Name:	ldh
Synonyms:	ldhL

Organism

Lactobacillus helveticus

Taxonomic identifier

1587 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Enzyme regulation	Fructose-1,6-diphosphate-dependent. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer. HAMAP MF_00488
Subcellular location	Cytoplasm HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	L-lactate dehydrogenase HAMAP MF_00488		PRO_0000168348
Regions
	Nucleotide binding	16 – 44	29	NAD By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	124	1	NAD or substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O32765-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 835E52465AA4C35A	FASTA	323	35,111
	10 20 30 40 50 60 MAREEKPRKV ILVGDGAVGS TFAFSMVQQG IAEELGIIDI AKEHVEGDAI DLADATPWTS 70 80 90 100 110 120 PKNIYAADYP DCKDADLVVI TAGAPQKPGE TRLDLVNKNL KILSSIVEPV VESGFEGIFL 130 140 150 160 170 180 VVANPVDILT HATWRMSGFP KDRVIGSGTS LDTGRLQKVI GKMENVDPSS VNAYMLGEHG 190 200 210 220 230 240 DTEFPAWSYN NVAGVKVADW VKAHNMPESK LEDIHQEVKD MAYDIINKKG ATFYGIGTAS 250 260 270 280 290 300 AMIAKAILND EHRVLPLSVP MDGEYGLHDL HIGTPAVVGR KGLEQVIEMP LSDKEQELMT 310 320 ASADQLKKVM DKAFKETGVK VRQ

References

[1]

"Molecular genetic characterization of the L-lactate dehydrogenase gene (ldhL) of Lactobacillus helveticus and biochemical characterization of the enzyme." Savijoki K., Palva A. Appl. Environ. Microbiol. 63:2850-2856(1997) [PubMed: 9212432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: 53/7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z81318 Genomic DNA. Translation: CAB03618.1.
3D structure databases
SMR	O32765. Positions 8-316.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.27. 39238.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH_LACHE

Accession

Primary (citable) accession number: O32765

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)