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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Lactobacillus helveticus (Lactobacillus suntoryeus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation1 Publication

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation1 Publication

Enzyme regulationi

Under neutral conditions, the reaction is stimulated 4-fold by fructose 1,6-bisphosphate (FBP), however the L-lactate dehydrogenase is a nonallosteric enzyme. Calcium and zinc ions at 1 mM stimulate the activity almost 2-fold. Weakly inhibited by cadmium, cobalt and copper ions.1 Publication

Kineticsi

Kcat is 643 sec(-1) for pyruvate as substrate.1 Publication
  1. KM=0.25 mM for pyruvate1 Publication

    pH dependencei

    Optimum pH is 5.1 Publication

    Pathwayi: pyruvate fermentation to lactate

    This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
    This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei18NAD; via amide nitrogenUniRule annotation1
    Binding sitei39NADUniRule annotation1
    Binding sitei69NADUniRule annotation1
    Binding sitei86SubstrateUniRule annotation1
    Binding sitei92SubstrateUniRule annotation1
    Binding sitei105NADUniRule annotation1
    Binding sitei147NADUniRule annotation1
    Active sitei179Proton acceptorUniRule annotation1
    Binding sitei232SubstrateUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi83 – 84NADUniRule annotation2
    Nucleotide bindingi122 – 124NADUniRule annotation3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    LigandNAD

    Enzyme and pathway databases

    SABIO-RKO32765
    UniPathwayiUPA00554; UER00611

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-lactate dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.27UniRule annotation1 Publication)
    Short name:
    L-LDH1 PublicationUniRule annotation
    Gene namesi
    Name:ldhUniRule annotation
    Synonyms:ldhL1 Publication
    OrganismiLactobacillus helveticus (Lactobacillus suntoryeus)
    Taxonomic identifieri1587 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001683482 – 323L-lactate dehydrogenaseAdd BLAST322

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei223PhosphotyrosineUniRule annotation1

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO32765
    SMRiO32765
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni124 – 127Substrate bindingUniRule annotation4
    Regioni152 – 155Substrate bindingUniRule annotation4

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. LDH family.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4105C80 Bacteria
    COG0039 LUCA

    Family and domain databases

    Gene3Di3.90.110.10, 1 hit
    HAMAPiMF_00488 Lactate_dehydrog, 1 hit
    InterProiView protein in InterPro
    IPR001557 L-lactate/malate_DH
    IPR011304 L-lactate_DH
    IPR018177 L-lactate_DH_AS
    IPR022383 Lactate/malate_DH_C
    IPR001236 Lactate/malate_DH_N
    IPR015955 Lactate_DH/Glyco_Ohase_4_C
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF02866 Ldh_1_C, 1 hit
    PF00056 Ldh_1_N, 1 hit
    PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
    PRINTSiPR00086 LLDHDRGNASE
    SUPFAMiSSF51735 SSF51735, 1 hit
    SSF56327 SSF56327, 1 hit
    TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
    PROSITEiView protein in PROSITE
    PS00064 L_LDH, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O32765-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAREEKPRKV ILVGDGAVGS TFAFSMVQQG IAEELGIIDI AKEHVEGDAI
    60 70 80 90 100
    DLADATPWTS PKNIYAADYP DCKDADLVVI TAGAPQKPGE TRLDLVNKNL
    110 120 130 140 150
    KILSSIVEPV VESGFEGIFL VVANPVDILT HATWRMSGFP KDRVIGSGTS
    160 170 180 190 200
    LDTGRLQKVI GKMENVDPSS VNAYMLGEHG DTEFPAWSYN NVAGVKVADW
    210 220 230 240 250
    VKAHNMPESK LEDIHQEVKD MAYDIINKKG ATFYGIGTAS AMIAKAILND
    260 270 280 290 300
    EHRVLPLSVP MDGEYGLHDL HIGTPAVVGR KGLEQVIEMP LSDKEQELMT
    310 320
    ASADQLKKVM DKAFKETGVK VRQ
    Length:323
    Mass (Da):35,111
    Last modified:January 1, 1998 - v1
    Checksum:i835E52465AA4C35A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z81318 Genomic DNA Translation: CAB03618.1
    RefSeqiWP_012211363.1, NZ_CP020029.1

    Similar proteinsi

    Entry informationi

    Entry nameiLDH_LACHE
    AccessioniPrimary (citable) accession number: O32765
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2001
    Last sequence update: January 1, 1998
    Last modified: May 23, 2018
    This is version 105 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

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