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O32740

- RBL1_RHOCB

UniProt

O32740 - RBL1_RHOCB

Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161Substrate; in homodimeric partnerUniRule annotation
    Binding sitei166 – 1661SubstrateUniRule annotation
    Active sitei168 – 1681Proton acceptorUniRule annotation
    Binding sitei170 – 1701SubstrateUniRule annotation
    Metal bindingi194 – 1941Magnesium; via carbamate groupUniRule annotation
    Metal bindingi196 – 1961MagnesiumUniRule annotation
    Metal bindingi197 – 1971MagnesiumUniRule annotation
    Active sitei287 – 2871Proton acceptorUniRule annotation
    Binding sitei288 – 2881SubstrateUniRule annotation
    Binding sitei320 – 3201SubstrateUniRule annotation
    Sitei327 – 3271Transition state stabilizerUniRule annotation
    Binding sitei372 – 3721SubstrateUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. monooxygenase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. reductive pentose-phosphate cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Calvin cycle, Carbon dioxide fixation, Photosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRCAP272942:GJIY-590-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCO large subunitUniRule annotation
    Gene namesi
    Name:cbbLUniRule annotation
    Synonyms:cbbL1
    Ordered Locus Names:RCAP_rcc00579
    OrganismiRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
    Taxonomic identifieri272942 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
    ProteomesiUP000002361: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Ribulose bisphosphate carboxylase large chainPRO_0000062645Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei194 – 1941N6-carboxylysineUniRule annotation

    Proteomic databases

    PRIDEiO32740.

    Interactioni

    Subunit structurei

    Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliO32740.
    SMRiO32740. Positions 17-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000230831.
    KOiK01601.
    OMAiMFKRAEY.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01338. RuBisCO_L_type1.
    InterProiIPR020878. RuBisCo_large_chain_AS.
    IPR020888. RuBisCO_lsu.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O32740-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAKTYDAGV KDYRSIYWEP QYQVKDSDIL AVFKVVPQPG VSREEAAAAV    50
    AAESSTATWT TVWTDLLTDL DYYKGRAYAI EDVPGSDEAF YAFIAYPMDL 100
    FEEGSVVNVF TSLVGNVFGF KAVRALRLED VRFPLWFVMT CPGAPHGMKV 150
    ERDLLDKYGR PLLGCTIKPK LGLAAKNYGR AVYECLRGGL DFTKDDENVN 200
    SQPFLRWRDR FLFCQEAIQK AEAETGERKG HYMNVTAGTM EEIYERAEFA 250
    KEIGTPIIMS DYLTVGWAAH TSLSRWCRKN GMLLHVHRAM HAVMDRNPNH 300
    GINFRVLAKI LRLMGGDHLH SGTVVGKLEG DREATIGWIN LLRDRFIKAD 350
    RSRGIFFDQD WGPQPGLFPV ASGGIHVWHM PALVSIFGND SVLQFGGGTL 400
    GHPWGNAAGA CANRVALEAC VQARNEGRHL EKEGKEILTK AAQSSPELRM 450
    AMETWKEIKF EFDTVDKLDV QHR 473
    Length:473
    Mass (Da):53,037
    Last modified:January 1, 1998 - v1
    Checksum:i9F2C3173618B9958
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L82000 Genomic DNA. Translation: AAC37141.1.
    CP001312 Genomic DNA. Translation: ADE84344.1.
    RefSeqiWP_013066323.1. NC_014034.1.
    YP_003576751.1. NC_014034.1.

    Genome annotation databases

    EnsemblBacteriaiADE84344; ADE84344; RCAP_rcc00579.
    GeneIDi9003408.
    KEGGircp:RCAP_rcc00579.
    PATRICi35501218. VBIRhoCap134200_0588.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L82000 Genomic DNA. Translation: AAC37141.1 .
    CP001312 Genomic DNA. Translation: ADE84344.1 .
    RefSeqi WP_013066323.1. NC_014034.1.
    YP_003576751.1. NC_014034.1.

    3D structure databases

    ProteinModelPortali O32740.
    SMRi O32740. Positions 17-460.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O32740.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE84344 ; ADE84344 ; RCAP_rcc00579 .
    GeneIDi 9003408.
    KEGGi rcp:RCAP_rcc00579.
    PATRICi 35501218. VBIRhoCap134200_0588.

    Phylogenomic databases

    HOGENOMi HOG000230831.
    KOi K01601.
    OMAi MFKRAEY.

    Enzyme and pathway databases

    BioCyci RCAP272942:GJIY-590-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01338. RuBisCO_L_type1.
    InterProi IPR020878. RuBisCo_large_chain_AS.
    IPR020888. RuBisCO_lsu.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rhodobacter capsulatus genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (cbbLS) and neighbouring genes were acquired by a horizontal gene transfer."
      Paoli G.C., Soyer F., Shively J., Tabita F.R.
      Microbiology 144:219-227(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
    2. "Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003."
      Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R.
      J. Bacteriol. 192:3545-3546(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
    3. "Expression of the cbbLcbbS and cbbM genes and distinct organization of the cbb Calvin cycle structural genes of Rhodobacter capsulatus."
      Paoli G.C., Morgan N.S., Tabita F.R., Shively J.M.
      Arch. Microbiol. 164:396-405(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: OPERON ORGANIZATION.
      Strain: ATCC BAA-309 / NBRC 16581 / SB1003.

    Entry informationi

    Entry nameiRBL1_RHOCB
    AccessioniPrimary (citable) accession number: O32740
    Secondary accession number(s): D5ANJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3