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O32728 (O32728_GEOSE) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
RNA polymerase sigma factor RuleBase RU000715
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus) EMBL AAB81194.1
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released By similarity. RuleBase RU000715

Sequence similarities

Belongs to the sigma-70 factor family. RuleBase RU000715

Binary interactions

With

Entry

#Exp.

IntAct

Notes

spoIIABO327275EBI-1033248,EBI-1033242

Sequences

Sequence LengthMass (Da)Tools
O32728 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: D0B9CBC7DDE41465

FASTA25028,775
        10         20         30         40         50         60 
MDVDVKQGQS PIKDQEMKEL IRRSQEGDQE ARDEIIEKNM RLVWSVVQRF LNRGYEADDL 

        70         80         90        100        110        120 
FQIGCIGLLK SVDKFDLSYD VKFSTYAVPM IIGEIQRFLR DDGTVKVSRS LKEMGNKIRK 

       130        140        150        160        170        180 
AKDELSKTRG RSPTITEIAD HLGISPEDVV LAQEAVRLPT SIHETVYEND GDPITLLDQI 

       190        200        210        220        230        240 
ADADEASWFD KIALKKAIEE LDERERLIVY LRYYRDRTQS EVASRLGISQ VQVSRLEKKI 

       250 
LQHIKDKMDG 

« Hide

References

[1]"Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species."
Park S.G., Yudkin M.D.
Gene 194:25-33(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Crystal structure of the Bacillus stearothermophilus anti-sigma factor SpoIIAB with the sporulation sigma factor sigmaF."
Campbell E.A., Masuda S., Sun J.L., Muzzin O., Olson C.A., Wang S., Darst S.A.
Cell 108:795-807(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 7-245.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L47360 Genomic DNA. Translation: AAB81194.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1L0OX-ray2.90C7-245[»]
ProteinModelPortalO32728.
SMRO32728. Positions 102-158.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO32728. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.10. 2 hits.
InterProIPR014284. RNA_pol_sigma-70_dom.
IPR014322. RNA_pol_sigma-B/F/G.
IPR014236. RNA_pol_sigma-F.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSPR00046. SIGMA70FCT.
SUPFAMSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsTIGR02980. SigBFG. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
TIGR02885. spore_sigF. 1 hit.
PROSITEPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO32728.

Entry information

Entry nameO32728_GEOSE
AccessionPrimary (citable) accession number: O32728
Entry history
Integrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)