O32728 (O32728_GEOSE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Recommended name: RNA polymerase sigma factor RuleBase RU000715
Organism	Geobacillus stearothermophilus (Bacillus stearothermophilus) EMBL AAB81194.1
Taxonomic identifier	1422 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released By similarity. RuleBase RU000715
Sequence similarities	Belongs to the sigma-70 factor family. RuleBase RU000715

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding SAAS SAAS007627 RuleBase RU000715
Molecular function	Sigma factor SAAS SAAS007627 RuleBase RU000715
Technical term	3D-structure PDB 1L0O
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
spoIIAB	O32727	5	EBI-1033248,EBI-1033242

Sequences

	Sequence		Length	Mass (Da)
	O32728 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D0B9CBC7DDE41465	FASTA	250	28,775
	10 20 30 40 50 60 MDVDVKQGQS PIKDQEMKEL IRRSQEGDQE ARDEIIEKNM RLVWSVVQRF LNRGYEADDL 70 80 90 100 110 120 FQIGCIGLLK SVDKFDLSYD VKFSTYAVPM IIGEIQRFLR DDGTVKVSRS LKEMGNKIRK 130 140 150 160 170 180 AKDELSKTRG RSPTITEIAD HLGISPEDVV LAQEAVRLPT SIHETVYEND GDPITLLDQI 190 200 210 220 230 240 ADADEASWFD KIALKKAIEE LDERERLIVY LRYYRDRTQS EVASRLGISQ VQVSRLEKKI 250 LQHIKDKMDG

References

[1]	"Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species." Park S.G., Yudkin M.D. Gene 194:25-33(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Crystal structure of the Bacillus stearothermophilus anti-sigma factor SpoIIAB with the sporulation sigma factor sigmaF." Campbell E.A., Masuda S., Sun J.L., Muzzin O., Olson C.A., Wang S., Darst S.A. Cell 108:795-807(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 7-245.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L47360 Genomic DNA. Translation: AAB81194.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1L0O	X-ray	2.90	C	7-245	[»]

ProteinModelPortal

O32728.

SMR

O32728. Positions 102-158.

ModBase

Search...

Protein-protein interaction databases

IntAct

O32728. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.10.10. 2 hits.

InterPro

IPR014284. RNA_pol_sigma-70_dom.
IPR014322. RNA_pol_sigma-B/F/G.
IPR014236. RNA_pol_sigma-F.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]

PRINTS

PR00046. SIGMA70FCT.

SUPFAM

SSF88946. Sigma_r2. 1 hit.
SSF88659. Sigma_r3_r4. 2 hits.

TIGRFAMs

TIGR02980. SigBFG. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
TIGR02885. spore_sigF. 1 hit.

PROSITE

PS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O32728.

Entry information

Entry name

O32728_GEOSE

Accession

Primary (citable) accession number: O32728

Entry history

Integrated into UniProtKB/TrEMBL:	January 1, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)