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Protein

RNA polymerase sigma factor

Gene
N/A
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Sigma factorUniRule annotationSAAS annotation

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factorUniRule annotation
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
spoIIABO327275EBI-1033248,EBI-1033242

Protein-protein interaction databases

IntActiO32728. 1 interaction.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L0OX-ray2.90C7-245[»]
ProteinModelPortaliO32728.
SMRiO32728. Positions 102-158.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO32728.

Family & Domainsi

Sequence similaritiesi

Belongs to the sigma-70 factor family.UniRule annotation

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR014322. RNA_pol_sigma-B/F/G.
IPR014236. RNA_pol_sigma-F.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR016263. RNA_pol_sigma_factor.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PIRSFiPIRSF000770. RNA_pol_sigma-SigE/K. 1 hit.
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02980. SigBFG. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
TIGR02885. spore_sigF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVDVKQGQS PIKDQEMKEL IRRSQEGDQE ARDEIIEKNM RLVWSVVQRF
60 70 80 90 100
LNRGYEADDL FQIGCIGLLK SVDKFDLSYD VKFSTYAVPM IIGEIQRFLR
110 120 130 140 150
DDGTVKVSRS LKEMGNKIRK AKDELSKTRG RSPTITEIAD HLGISPEDVV
160 170 180 190 200
LAQEAVRLPT SIHETVYEND GDPITLLDQI ADADEASWFD KIALKKAIEE
210 220 230 240 250
LDERERLIVY LRYYRDRTQS EVASRLGISQ VQVSRLEKKI LQHIKDKMDG
Length:250
Mass (Da):28,775
Last modified:January 1, 1998 - v1
Checksum:iD0B9CBC7DDE41465
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47360 Genomic DNA. Translation: AAB81194.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47360 Genomic DNA. Translation: AAB81194.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L0OX-ray2.90C7-245[»]
ProteinModelPortaliO32728.
SMRiO32728. Positions 102-158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO32728. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO32728.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR014322. RNA_pol_sigma-B/F/G.
IPR014236. RNA_pol_sigma-F.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR016263. RNA_pol_sigma_factor.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PIRSFiPIRSF000770. RNA_pol_sigma-SigE/K. 1 hit.
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02980. SigBFG. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
TIGR02885. spore_sigF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species."
    Park S.G., Yudkin M.D.
    Gene 194:25-33(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Crystal structure of the Bacillus stearothermophilus anti-sigma factor SpoIIAB with the sporulation sigma factor sigmaF."
    Campbell E.A., Masuda S., Sun J.L., Muzzin O., Olson C.A., Wang S., Darst S.A.
    Cell 108:795-807(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 7-245.

Entry informationi

Entry nameiO32728_GEOSE
AccessioniPrimary (citable) accession number: O32728
Entry historyi
Integrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: April 1, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.