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O32728

- O32728_GEOSE

UniProt

O32728 - O32728_GEOSE

Protein

RNA polymerase sigma factor

Gene
N/A
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.UniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. sigma factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA-templated transcription, initiation Source: InterPro

    Keywords - Molecular functioni

    Sigma factorUniRule annotationSAAS annotation

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-bindingUniRule annotationSAAS annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase sigma factorUniRule annotation
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    spoIIABO327275EBI-1033248,EBI-1033242

    Protein-protein interaction databases

    IntActiO32728. 1 interaction.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1L0OX-ray2.90C7-245[»]
    ProteinModelPortaliO32728.
    SMRiO32728. Positions 102-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO32728.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the sigma-70 factor family.UniRule annotation

    Family and domain databases

    Gene3Di1.10.10.10. 2 hits.
    InterProiIPR014284. RNA_pol_sigma-70_dom.
    IPR014322. RNA_pol_sigma-B/F/G.
    IPR014236. RNA_pol_sigma-F.
    IPR000943. RNA_pol_sigma70.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view]
    PRINTSiPR00046. SIGMA70FCT.
    SUPFAMiSSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsiTIGR02980. SigBFG. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    TIGR02885. spore_sigF. 1 hit.
    PROSITEiPS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O32728-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVDVKQGQS PIKDQEMKEL IRRSQEGDQE ARDEIIEKNM RLVWSVVQRF    50
    LNRGYEADDL FQIGCIGLLK SVDKFDLSYD VKFSTYAVPM IIGEIQRFLR 100
    DDGTVKVSRS LKEMGNKIRK AKDELSKTRG RSPTITEIAD HLGISPEDVV 150
    LAQEAVRLPT SIHETVYEND GDPITLLDQI ADADEASWFD KIALKKAIEE 200
    LDERERLIVY LRYYRDRTQS EVASRLGISQ VQVSRLEKKI LQHIKDKMDG 250
    Length:250
    Mass (Da):28,775
    Last modified:January 1, 1998 - v1
    Checksum:iD0B9CBC7DDE41465
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47360 Genomic DNA. Translation: AAB81194.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47360 Genomic DNA. Translation: AAB81194.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1L0O X-ray 2.90 C 7-245 [» ]
    ProteinModelPortali O32728.
    SMRi O32728. Positions 102-158.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O32728. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O32728.

    Family and domain databases

    Gene3Di 1.10.10.10. 2 hits.
    InterProi IPR014284. RNA_pol_sigma-70_dom.
    IPR014322. RNA_pol_sigma-B/F/G.
    IPR014236. RNA_pol_sigma-F.
    IPR000943. RNA_pol_sigma70.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view ]
    PRINTSi PR00046. SIGMA70FCT.
    SUPFAMi SSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsi TIGR02980. SigBFG. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    TIGR02885. spore_sigF. 1 hit.
    PROSITEi PS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species."
      Park S.G., Yudkin M.D.
      Gene 194:25-33(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Crystal structure of the Bacillus stearothermophilus anti-sigma factor SpoIIAB with the sporulation sigma factor sigmaF."
      Campbell E.A., Masuda S., Sun J.L., Muzzin O., Olson C.A., Wang S., Darst S.A.
      Cell 108:795-807(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 7-245.

    Entry informationi

    Entry nameiO32728_GEOSE
    AccessioniPrimary (citable) accession number: O32728
    Entry historyi
    Integrated into UniProtKB/TrEMBL: January 1, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3