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O32727

- SP2AB_GEOSE

UniProt

O32727 - SP2AB_GEOSE

Protein

Anti-sigma F factor

Gene

spoIIAB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: UniProtKB-KW
    4. sigma factor antagonist activity Source: InterPro

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: InterPro
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Sporulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anti-sigma F factorUniRule annotation (EC:2.7.11.1UniRule annotation)
    Alternative name(s):
    Stage II sporulation protein ABUniRule annotation
    Gene namesi
    Name:spoIIABUniRule annotation
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 146146Anti-sigma F factorPRO_0000203560Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    O327285EBI-1033242,EBI-1033248
    spoIIAAO327264EBI-1033242,EBI-1039369

    Protein-protein interaction databases

    IntActiO32727. 2 interactions.

    Structurei

    Secondary structure

    1
    146
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 119
    Helixi15 – 2713
    Helixi28 – 303
    Helixi34 – 5320
    Beta strandi56 – 583
    Beta strandi62 – 7110
    Beta strandi74 – 818
    Helixi89 – 924
    Turni101 – 1044
    Helixi109 – 1168
    Beta strandi117 – 1259
    Turni126 – 1283
    Beta strandi129 – 1368

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1L0OX-ray2.90A/B1-142[»]
    1TH8X-ray2.40A1-136[»]
    1THNX-ray2.50A/C1-136[»]
    1TIDX-ray2.50A/C1-136[»]
    1TILX-ray2.70A/C/E1-146[»]
    ProteinModelPortaliO32727.
    SMRiO32727. Positions 1-136.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO32727.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the anti-sigma-factor family.UniRule annotation

    Family and domain databases

    Gene3Di3.30.565.10. 1 hit.
    HAMAPiMF_00637. Anti_sigma_F.
    InterProiIPR010194. Anti-sigma_F.
    IPR003594. HATPase_ATP-bd.
    [Graphical view]
    PfamiPF02518. HATPase_c. 1 hit.
    [Graphical view]
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF55874. SSF55874. 1 hit.
    TIGRFAMsiTIGR01925. spIIAB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O32727-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRNEMHLQFS ARSENESFAR VTVAAFVAQL DPTTDELTEI KTVVSEAVTN    50
    AIIHGYNNDP NGIVSISVII EDGVVHLTVR DEGVGIPDIE EARQPLFTTK 100
    PELERSGMGF TIMENFMDEV IVESEVNKGT TVYLKKAYCE KQTLCN 146
    Length:146
    Mass (Da):16,239
    Last modified:January 1, 1998 - v1
    Checksum:iB3F1BD38A9044416
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47360 Genomic DNA. Translation: AAB81193.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47360 Genomic DNA. Translation: AAB81193.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1L0O X-ray 2.90 A/B 1-142 [» ]
    1TH8 X-ray 2.40 A 1-136 [» ]
    1THN X-ray 2.50 A/C 1-136 [» ]
    1TID X-ray 2.50 A/C 1-136 [» ]
    1TIL X-ray 2.70 A/C/E 1-146 [» ]
    ProteinModelPortali O32727.
    SMRi O32727. Positions 1-136.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O32727. 2 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O32727.

    Family and domain databases

    Gene3Di 3.30.565.10. 1 hit.
    HAMAPi MF_00637. Anti_sigma_F.
    InterProi IPR010194. Anti-sigma_F.
    IPR003594. HATPase_ATP-bd.
    [Graphical view ]
    Pfami PF02518. HATPase_c. 1 hit.
    [Graphical view ]
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55874. SSF55874. 1 hit.
    TIGRFAMsi TIGR01925. spIIAB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species."
      Park S.G., Yudkin M.D.
      Gene 194:25-33(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 12980 / DSM 22 / JCM 2501 / NBRC 12550 / NCIMB 8923 / NCTC 10339 / VKM B-510.

    Entry informationi

    Entry nameiSP2AB_GEOSE
    AccessioniPrimary (citable) accession number: O32727
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3