Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O32727 (SP2AB_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anti-sigma F factor

EC=2.7.11.1
Alternative name(s):
Stage II sporulation protein AB
Gene names
Name:spoIIAB
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length146 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition By similarity. HAMAP-Rule MF_00637

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_00637

Sequence similarities

Belongs to the anti-sigma-factor family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 146146Anti-sigma F factor HAMAP-Rule MF_00637
PRO_0000203560

Secondary structure

....................... 146
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O32727 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: B3F1BD38A9044416

FASTA14616,239
        10         20         30         40         50         60 
MRNEMHLQFS ARSENESFAR VTVAAFVAQL DPTTDELTEI KTVVSEAVTN AIIHGYNNDP 

        70         80         90        100        110        120 
NGIVSISVII EDGVVHLTVR DEGVGIPDIE EARQPLFTTK PELERSGMGF TIMENFMDEV 

       130        140 
IVESEVNKGT TVYLKKAYCE KQTLCN 

« Hide

References

[1]"Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species."
Park S.G., Yudkin M.D.
Gene 194:25-33(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12980 / DSM 22 / JCM 2501 / NBRC 12550 / NCIMB 8923 / NCTC 10339 / VKM B-510.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L47360 Genomic DNA. Translation: AAB81193.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1L0OX-ray2.90A/B1-142[»]
1TH8X-ray2.40A1-136[»]
1THNX-ray2.50A/C1-136[»]
1TIDX-ray2.50A/C1-136[»]
1TILX-ray2.70A/C/E1-146[»]
ProteinModelPortalO32727.
SMRO32727. Positions 1-136.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO32727. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.565.10. 1 hit.
HAMAPMF_00637. Anti_sigma_F.
InterProIPR010194. Anti-sigma_F.
IPR003594. HATPase_ATP-bd.
[Graphical view]
PfamPF02518. HATPase_c. 1 hit.
[Graphical view]
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMSSF55874. SSF55874. 1 hit.
TIGRFAMsTIGR01925. spIIAB. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO32727.

Entry information

Entry nameSP2AB_GEOSE
AccessionPrimary (citable) accession number: O32727
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references